A Method for Studying Effects on Lignin-Polysaccharide Networks during Biological Degradation and Technical Processes of Wood
2016 (English)In: BioResources, ISSN 1930-2126, E-ISSN 1930-2126, Vol. 11, no 1, 1307-1318 p.Article in journal (Refereed) Published
Woody tissues consist primarily of a mixture of cellulose, hemicelluloses, and lignin. Covalent bonds between lignin and polysaccharides likely play a central role in determining the mechanical and physical properties of wood. Intact and defined lignin-polysaccharide networks have not been isolated in large quantities because of the recalcitrance of lignin, which demands harsh chemical treatments that alter its structure. This report presents a method for preparing large quantities of lignin-polysaccharide networks similar to those naturally present in wood based on the enzymatic oxidation of hemicellulose from Norway spruce. Fungal enzymes produced from various carbon sources were used to depolymerize these networks. The method was used for simulating "enzyme mining" - a concept in biorefineries, giving a possible explanation for its mechanisms.
Place, publisher, year, edition, pages
NORTH CAROLINA STATE UNIV , 2016. Vol. 11, no 1, 1307-1318 p.
Lignin carbohydrate complexes, Wood, Model system, Biorefinery, Biodegradation
IdentifiersURN: urn:nbn:se:kth:diva-182267DOI: 10.15376/biores.11.1.1307-1318ISI: 000367732700103OAI: oai:DiVA.org:kth-182267DiVA: diva2:904179
FunderKnut and Alice Wallenberg Foundation
QC 201602232016-02-182016-02-182016-02-23Bibliographically approved