Change search
ReferencesLink to record
Permanent link

Direct link
The Human Endometrium-Specific Proteome Defined by Transcriptomics and Antibody-Based Profiling
KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-0198-7137
Show others and affiliations
2015 (English)In: Omics, ISSN 1536-2310, E-ISSN 1557-8100, Vol. 19, no 11, 659-668 p.Article in journal (Refereed) PublishedText
Abstract [en]

The human uterus includes the complex endometrial mucosa, the endometrium that undergoes dynamic, hormone-dependent alterations throughout the life of fertile females. Here we have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to analyze gene expression patterns in the normal endometrium. Human endometrial tissues from five women were used for deep sequencing (RNA-Seq). The mRNA and protein expression data from the endometrium were compared to 31 (RNA) and 44 (protein) other normal tissue types, to identify genes with elevated expression in the endometrium and to localize the expression of corresponding proteins at a cellular resolution. Based on the expression levels of transcripts, we could classify all putative human protein coding genes into categories defined by expression patterns and found altogether 101 genes that showed an elevated pattern of expression in the endometrium, with only four genes showing more than five-fold higher expression levels in the endometrium compared to other tissues. In conclusion, our analysis based on transcriptomics and antibody-based protein profiling reports here comprehensive lists of genes with elevated expression levels in the endometrium, providing important starting points for a better molecular understanding of human reproductive biology and disease.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2015. Vol. 19, no 11, 659-668 p.
Keyword [en]
messenger RNA, proteome, adult, antibody based profiling, Article, endometrium, female, gene expression, gene expression profiling, gene identification, genetic procedures, genome analysis, human, human tissue, immunohistochemistry, priority journal, protein expression, RNA sequence, transcriptomics
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Genetics
URN: urn:nbn:se:kth:diva-181225DOI: 10.1089/omi.2015.0115ISI: 000364547500001ScopusID: 2-s2.0-84947058693OAI: diva2:904332

QC 20160218

Available from: 2016-02-18 Created: 2016-01-29 Last updated: 2016-02-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Sjöstedt, EvelinaFagerberg, LinnHallström, Björn M.Uhlén, Mathias
By organisation
Science for Life Laboratory, SciLifeLabProteomics and Nanobiotechnology
In the same journal
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Genetics

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 18 hits
ReferencesLink to record
Permanent link

Direct link