Change search
ReferencesLink to record
Permanent link

Direct link
Zbasic: a purification tag for selective ion-exchange recovery
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0003-0140-419X
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0002-6104-6446
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0003-0605-8417
2014 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1129, 197-204 p.Article in journal (Refereed) Published
Abstract [en]

A positively charged protein domain, denoted Zbasic, can be used as a general purification tag for purification of recombinantly produced target proteins by cation-exchange chromatography. The Zbasic domain is constructed from the Protein A-derived domain and engineered to be highly charged, which allows selective capture on a cation exchanger at physiological pH values. Moreover, Zbasic is selective also under denaturing conditions and can be used for purification of proteins solubilized from inclusion bodies. Zbasic can then be used as a flexible linker to the cation-exchange resin and thereby allow solid-phase refolding of the target protein.Herein, protocols for purification of soluble Zbasic-tagged fusion proteins, as well as for integrated purification and solid-phase refolding of insoluble fusion proteins, are described. In addition, a procedure for enzymatic tag removal and recovery of native target protein is outlined.

Place, publisher, year, edition, pages
2014. Vol. 1129, 197-204 p.
National Category
Biochemistry and Molecular Biology
Research subject
Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-182468OAI: oai:DiVA.org:kth-182468DiVA: diva2:904566
Note

QC 20160303

Available from: 2016-02-19 Created: 2016-02-19 Last updated: 2016-03-03Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Hedhammar, MyNilvebrant, JohanHober, Sophia
By organisation
Protein Technology
In the same journal
Methods in Molecular Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 15 hits
ReferencesLink to record
Permanent link

Direct link