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Structure of DPPC-hyaluronan interfacial layers - effects of molecular weight and ion composition
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2016 (English)In: Soft Matter, ISSN 1744-683X, E-ISSN 1744-6848, Vol. 12, no 3, 729-740 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Hyaluronan and phospholipids play an important role in lubrication in articular joints and provide in combination with glycoproteins exceptionally low friction coefficients. We have investigated the structural organization of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) Langmuir layers at the solution-air interface at different length scales with respect to the adsorption of hyaluronan (HA). This allows us to assemble a comprehensive picture of the adsorption and the resulting structures, and how they are affected by the molecular weight of HA and the presence of calcium ions. Brewster angle microscopy and grazing incident diffraction were used to determine the lateral structure at the micro- and macro scale. The data reveals an influence of HA on both the macro and micro structure of the DPPC Langmuir layer, and that the strength of this effect increases with decreasing molecular weight of HA and in presence of calcium ions. Furthermore, from X-ray reflectivity measurements we conclude that HA adsorbs to the hydrophilic part of DPPC, but data also suggest that two types of interfacial structures are formed at the interface. We argue that hydrophobic forces and electrostatic interactions play important rules for the association between DPPC and HA. Surface pressure area isotherms were used to determine the influence of HA on the phase behavior of DPPC while electrophoretic mobility measurements were used to gain insight into the binding of calcium ions to DPPC vesicles and hyaluronan.

Place, publisher, year, edition, pages
Royal Society of Chemistry , 2016. Vol. 12, no 3, 729-740 p.
National Category
Materials Engineering Chemical Sciences Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-183346DOI: 10.1039/c5sm01708dISI: 000369746800010PubMedID: 26508354Scopus ID: 2-s2.0-84954134754OAI: oai:DiVA.org:kth-183346DiVA: diva2:909593
Note

QC 20160307

Available from: 2016-03-07 Created: 2016-03-07 Last updated: 2017-02-09Bibliographically approved
In thesis
1. Synergies in Biolubrication
Open this publication in new window or tab >>Synergies in Biolubrication
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The objective of this thesis was to advance understanding in the field of biolubrication, finding inspiration from the human synovial joints. This was addressed by investigating the association of key biolubricants and the resulting lubrication performance. Techniques employed during the course of this work were Atomic force microscopy (AFM), Quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray reflectivity (XRR).

Key synovial fluid and cartilage components like dipalmitoylphosphatidylcholine (DPPC), hyaluronan (HA), lubricin, and cartilage oligomeric matrix protein (COMP) have been used in the investigations. Focus was towards two lubrication couples; DPPC-hyaluronan and COMP-lubricin. DPPC-hyaluronan mixtures were probed on hydrophilic silica surfaces and COMP-lubricin association structures were explored on weakly hydrophobic poly (methyl methacrylate) (PMMA) surfaces.

Investigations of the COMP-lubricin pair revealed that individually these components are unable to reach desired lubrication. However in combination, COMP facilitates firm attachment of lubricin to the PMMA surface in a favourable confirmation that imparts low friction coefficient.

DPPC and hyaluronan combined impart lubrication advantage over lone DPPC bilayers. Hyaluronan provides a reservoir of DPPC on the surface and consequently self-healing ability.

Other factors like temperature, presence of calcium ions, molecular weight of hyaluronan, and pressure were also explored. DPPC bilayers at higher temperature had higher load bearing capacity. Association between DPPC Langmuir layers and hyaluronan was enhanced in the presence of calcium ions, and lower molecular weight hyaluronan had a stronger tendency to bind to DPPC. At high pressures, DPPC-hyaluronan layers were more stable compared to lone DPPC bilayers.

Place, publisher, year, edition, pages
Sweden: KTH Royal Institute of Technology, 2017. 66 p.
Series
TRITA-CHE-Report, ISSN 1654-1081
Keyword
Biolubrication, Synergies, Adsorption, Surface Force, Friction, Load Bearing Capacity, Self Healing, Phospholipids, DPPC, Hyaluronan, COMP, Lubricin, QCM-D, AFM, XRR.
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-201219 (URN)978-91-7729-268-5 (ISBN)
Public defence
2017-03-17, Kollegiesalen, KTH, Brinellvägen 8, Stockholm, 10:00 (English)
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Supervisors
Note

QC 20170210

Available from: 2017-02-10 Created: 2017-02-09 Last updated: 2017-02-13Bibliographically approved

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