Change search
ReferencesLink to record
Permanent link

Direct link
Structure of DPPC-hyaluronan interfacial layers - effects of molecular weight and ion composition
Show others and affiliations
2016 (English)In: Soft Matter, ISSN 1744-683X, E-ISSN 1744-6848, Vol. 12, no 3, 729-740 p.Article in journal (Refereed) PublishedText
Abstract [en]

Hyaluronan and phospholipids play an important role in lubrication in articular joints and provide in combination with glycoproteins exceptionally low friction coefficients. We have investigated the structural organization of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) Langmuir layers at the solution-air interface at different length scales with respect to the adsorption of hyaluronan (HA). This allows us to assemble a comprehensive picture of the adsorption and the resulting structures, and how they are affected by the molecular weight of HA and the presence of calcium ions. Brewster angle microscopy and grazing incident diffraction were used to determine the lateral structure at the micro- and macro scale. The data reveals an influence of HA on both the macro and micro structure of the DPPC Langmuir layer, and that the strength of this effect increases with decreasing molecular weight of HA and in presence of calcium ions. Furthermore, from X-ray reflectivity measurements we conclude that HA adsorbs to the hydrophilic part of DPPC, but data also suggest that two types of interfacial structures are formed at the interface. We argue that hydrophobic forces and electrostatic interactions play important rules for the association between DPPC and HA. Surface pressure area isotherms were used to determine the influence of HA on the phase behavior of DPPC while electrophoretic mobility measurements were used to gain insight into the binding of calcium ions to DPPC vesicles and hyaluronan.

Place, publisher, year, edition, pages
Royal Society of Chemistry , 2016. Vol. 12, no 3, 729-740 p.
National Category
Materials Engineering Chemical Sciences Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-183346DOI: 10.1039/c5sm01708dISI: 000369746800010PubMedID: 26508354ScopusID: 2-s2.0-84954134754OAI: oai:DiVA.org:kth-183346DiVA: diva2:909593
Note

QC 20160307

Available from: 2016-03-07 Created: 2016-03-07 Last updated: 2016-03-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Raj, AkankshaAn, JunxueDedinaite, AndraClaesson, Per
By organisation
Surface and Corrosion Science
In the same journal
Soft Matter
Materials EngineeringChemical SciencesPhysical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 24 hits
ReferencesLink to record
Permanent link

Direct link