Visible-to-visible four-photon ultrahigh resolution microscopic imaging with 730-nm diode laser excited nanocrystals
2016 (English)In: Optics Express, ISSN 1094-4087, E-ISSN 1094-4087, Vol. 24, no 2, A302-A311 p.Article in journal (Refereed) PublishedText
Further development of multiphoton microscopic imaging is confronted with a number of limitations, including high-cost, high complexity and relatively low spatial resolution due to the long excitation wavelength. To overcome these problems, for the first time, we propose visible-to-visible four-photon ultrahigh resolution microscopic imaging by using a common cost-effective 730-nm laser diode to excite the prepared Nd3+-sensitized upconversion nanoparticles (Nd3+-UCNPs). An ordinary multiphoton scanning microscope system was built using a visible CW diode laser and the lateral imaging resolution as high as 161-nm was achieved via the four-photon upconversion process. The demonstrated large saturation excitation power for Nd3+-UCNPs would be more practical and facilitate the four-photon imaging in the application. A sample with fine structure was imaged to demonstrate the advantages of visible-to-visible four-photon ultrahigh resolution microscopic imaging with 730-nm diode laser excited nanocrystals. Combining the uniqueness of UCNPs, the proposed visible-to-visible four-photon imaging would be highly promising and attractive in the field of multiphoton imaging.
Place, publisher, year, edition, pages
Optical Society of America, 2016. Vol. 24, no 2, A302-A311 p.
Atom and Molecular Physics and Optics
IdentifiersURN: urn:nbn:se:kth:diva-183335DOI: 10.1364/OE.24.00A302ISI: 000369066300029PubMedID: 26832583ScopusID: 2-s2.0-84962054210OAI: oai:DiVA.org:kth-183335DiVA: diva2:910099
QC 201603082016-03-082016-03-072016-03-19Bibliographically approved