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Of Quantum Dots and Microbes: Smart materials for fluorescence based characterization of environmental microflora
Center of Excellence in Nanotechnology, Asian Institute of Technology,Thailand.ORCID iD: 0000-0002-0074-3504
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2013 (English)In: Proc. of the Intl. Conf. on Future Trends in Structural, Civil, Environmental and Mechanical Engineering – FTSCEM 2013, SEEK Digital Library , 2013, 61-65 p.Conference paper, Published paper (Refereed)
Abstract [en]

Manganese doped zinc sulfide (ZnS:Mn2+) quantum dots (QDs) were surface derivatized for use as versatile fluorescent reporters is the study of microorganisms of relevance in environmental bioprocesses. When intracellularly introduced into permeabilized target bacterial cells, mercaptoacetic acid (MAA) or dithiothreitol (DTT) treatedZnS:Mn2+QDs proved to be good visualization enhancers for morphological observations with epifluorescence microscopy. Alternatively, cell surface adhesion of chitosan capped ZnS:Mn2+QDs also led to a similar effect, namely of QD enhanced contrast of target cells from intrinsic background fluorescence. When DTT surface derivatized ZnS:Mn2+QDs were conjugated with oligonucleotide probes and used in fluorescent in situ hybridization (FISH) analysis, specific detection of bacterial strains representative ofsome proteobacterial classes was achieved.

Place, publisher, year, edition, pages
SEEK Digital Library , 2013. 61-65 p.
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:kth:diva-176595OAI: oai:DiVA.org:kth-176595DiVA: diva2:914304
Conference
Proc. of the Intl. Conf. on Future Trends in Structural, Civil, Environmental and Mechanical Engineering – FTSCEM 2013
Note

QC 20160330

Available from: 2016-03-23 Created: 2015-11-07 Last updated: 2017-06-02Bibliographically approved

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