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Antibodies as means for selective mass spectrometry
KTH, School of Biotechnology (BIO), Protein Technology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0003-0605-8417
2015 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376XArticle in journal (Refereed) Epub ahead of print
Abstract [en]

For protein analysis of biological samples, two major strategies are used today; mass spectrometry (MS) and antibody-based methods. Each strategy offers advantages and drawbacks. However, combining the two using an immunoenrichment step with MS analysis brings together the benefits of each method resulting in increased sensitivity, faster analysis and possibility of higher degrees of multiplexing. The immunoenrichment can be performed either on protein or peptide level and quantification standards can be added in order to enable determination of the absolute protein concentration in the sample. The combination of immunoenrichment and MS holds great promise for the future in both proteomics and clinical diagnostics. This review describes different setups of immunoenrichment coupled to mass spectrometry and how these can be utilized in various applications.

Place, publisher, year, edition, pages
Elsevier, 2015.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kth:diva-184210DOI: 10.1016/j.jchromb.2015.10.042PubMedID: 26565067Scopus ID: 2-s2.0-84964433642OAI: oai:DiVA.org:kth-184210DiVA: diva2:915599
Note

QP 2016

Available from: 2016-03-30 Created: 2016-03-30 Last updated: 2016-04-08Bibliographically approved

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