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Metagenomic analysis of bloodstream infections in patients with acute leukemia and therapy-induced neutropenia
KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 23532Article in journal (Refereed) Published
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Abstract [en]

Leukemic patients are often immunocompromised due to underlying conditions, comorbidities and the effects of chemotherapy, and thus at risk for developing systemic infections. Bloodstream infection (BSI) is a severe complication in neutropenic patients, and is associated with increased mortality. BSI is routinely diagnosed with blood culture, which only detects culturable pathogens. We analyzed 27 blood samples from 9 patients with acute leukemia and suspected BSI at different time points of their antimicrobial treatment using shotgun metagenomics sequencing in order to detect unculturable and non-bacterial pathogens. Our findings confirm the presence of bacterial, fungal and viral pathogens alongside antimicrobial resistance genes. Decreased white blood cell (WBC) counts were associated with the presence of microbial DNA, and was inversely proportional to the number of sequencing reads. This study could indicate the use of high-throughput sequencing for personalized antimicrobial treatments in BSIs.

Place, publisher, year, edition, pages
Nature Publishing Group, 2016. Vol. 6, 23532
Keyword [en]
Chemotherapeutic Control, Sepsis, Susceptibility, Microbiome, Resistance
National Category
Other Medical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-185359DOI: 10.1038/srep23532ISI: 000372516300001PubMedID: 26996149Scopus ID: 2-s2.0-84961753723OAI: oai:DiVA.org:kth-185359DiVA: diva2:920582
Funder
Swedish Research Council, 2012-3291Swedish Society of Medicine, SLS-408571
Note

QC 20160418

Available from: 2016-04-18 Created: 2016-04-18 Last updated: 2016-04-18Bibliographically approved

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