Squalene hopene cyclases: highly promiscuous and evolvable catalysts for stereoselective CC and CX bond formation
2013 (English)In: Current opinion in chemical biology, ISSN 1367-5931, E-ISSN 1879-0402, Vol. 17, no 2, 293-300 p.Article in journal (Refereed) Published
A review. We review here how the inherent promiscuous nature, as well as the evolvability of terpene cyclase enzymes enables new applications in chem. We mainly focus on squalene hopene cyclases, class II triterpene synthases that use a proton-initiated cationic polycyclization cascade to form carbopolycyclic products. We highlight recent findings to demonstrate that these enzymes are capable of activating different functionalities other than the traditional terminal isoprene CC-group as well as being compatible with a wide range of nucleophiles beyond the 'ene-functionality'. Thus, squalene hopene cyclases demonstrate a great potential to be used as a toolbox for general Bronsted acid catalysis.
Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 17, no 2, 293-300 p.
review squalene hopene cyclase promiscuous evolvable catalyst stereoselectivity
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-185568DOI: 10.1016/j.cbpa.2013.01.016ISI: 000318749500022ScopusID: 2-s2.0-84876731984OAI: oai:DiVA.org:kth-185568DiVA: diva2:922318
QC 201604292016-04-222016-04-222016-04-29Bibliographically approved