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Minimizing antibody cross-reactivity in multiplex detection of biomarkers in paper-based point-of-care assays
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-4856-860X
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
2016 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, no 15, 8195-8201 p.Article in journal (Refereed) PublishedText
Abstract [en]

Highly multiplexed immunoassays could allow convenient screening of hundreds or thousands of protein biomarkers simultaneously in a clinical sample such as serum or plasma, potentially allowing improved diagnostic accuracy and clinical management of many conditions such as autoimmune disorders, infections, and several cancers. Currently, antibody microarray-based tests are limited in part due to cross reactivity from detection antibody reagents. Here we present a strategy that reduces the cross-reactivity between nanoparticle-bound reporter antibodies through the application of ultrasound energy. By this concept, it was possible to achieve a sensitivity 10(3)-fold (5 pg mL(-1)) lower than when no ultrasound was applied (50 ng mL(-1)) for the simultaneous detection of three different antigens. The detection limits and variability achieved with this technique rival those obtained with other types of multiplex sandwich assays.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2016. Vol. 8, no 15, 8195-8201 p.
National Category
Chemical Sciences
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URN: urn:nbn:se:kth:diva-186575DOI: 10.1039/c5nr09207hISI: 000374159600047PubMedID: 27030365ScopusID: 2-s2.0-84964677942OAI: oai:DiVA.org:kth-186575DiVA: diva2:927953
Note

QC 20160513

Available from: 2016-05-13 Created: 2016-05-13 Last updated: 2016-05-16Bibliographically approved

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Dias, Jorge T.Lama, LaraGantelius, JesperAndersson-Svahn, Helene
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Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLab
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