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The importance of extracellular speciation and corrosion of copper nanoparticles on lung cell membrane integrity
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2100-8864
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2145-3650
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science. SP Technical Research Institute of Sweden, Sweden.ORCID iD: 0000-0001-7496-1101
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2016 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 141, 291-300 p.Article in journal (Refereed) PublishedText
Abstract [en]

Copper nanoparticles (Cu NPs) are increasingly used in various biologically relevant applications and products, e.g., due to their antimicrobial and catalytic properties. This inevitably demands for an improved understanding on their interactions and potential toxic effects on humans. The aim of this study was to investigate the corrosion of copper nanoparticles in various biological media and to elucidate the speciation of released copper in solution. Furthermore, reactive oxygen species (ROS) generation and lung cell (A549 type II) membrane damage induced by Cu NPs in the various media were studied. The used biological media of different complexity are of relevance for nanotoxicological studies: Dulbecco's modified eagle medium (DMEM), DMEM+ (includes fetal bovine serum), phosphate buffered saline (PBS), and PBS + histidine. The results show that both copper release and corrosion are enhanced in DMEM+, DMEM, and PBS + histidine compared with PBS alone. Speciation results show that essentially no free copper ions are present in the released fraction of Cu NPs in neither DMEM+, DMEM nor histidine, while labile Cu complexes form in PBS. The Cu NPs were substantially more membrane reactive in PBS compared to the other media and the NPs caused larger effects compared to the same mass of Cu ions. Similarly, the Cu NPs caused much more ROS generation compared to the released fraction only. Taken together, the results suggest that membrane damage and ROS formation are stronger induced by Cu NPs and by free or labile Cu ions/complexes compared with Cu bound to biomolecules.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 141, 291-300 p.
Keyword [en]
Copper nanoparticles, Nanotoxicity, Lung cells, Speciation, Corrosion, Membrane damage, DMEM, Equilibrium modeling, UV-vis spectroscopy, Polarography
National Category
Biophysics Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-186555DOI: 10.1016/j.colsurfb.2016.01.052ISI: 000374197700034PubMedID: 26859121ScopusID: 2-s2.0-84957022551OAI: oai:DiVA.org:kth-186555DiVA: diva2:927966
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QC 20160513

Available from: 2016-05-13 Created: 2016-05-13 Last updated: 2016-05-16Bibliographically approved

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Hedberg, JonasHedberg, YolandaBlomberg, EvaWallinder, Inger Odnevall
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