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Analysis of polyvinyl alcohol microbubbles in human blood plasma using capillary electrophoresis
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-2821-1462
KTH, School of Technology and Health (STH), Medical Engineering, Medical Imaging.ORCID iD: 0000-0002-4757-7232
KTH, School of Technology and Health (STH), Medical Engineering, Medical Imaging. Karolinska Institute, Sweden.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-3444-9987
2016 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 39, no 8, 1551-1558 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Recently, a new type of ultrasound contrast agent that consists of air-filled microbubbles stabilized with a shell of polyvinyl alcohol was developed. When superparamagnetic nanoparticles of iron oxide are incorporated in the polymer shell, a multimodal contrast agent can be obtained. The biodistribution and elimination pathways of the polyvinyl alcohol microbubbles are essential to investigate, which is limited with today's techniques. The aim of the present study was, therefore, to develop a method for qualitative and quantitative analysis of microbubbles in biological samples using capillary electrophoresis with ultraviolet detection. The analysis parameters were optimized to a wavelength at 260 nm and pH of the background electrolyte ranging between 11.9 and 12. Studies with high-intensity ultrasonication degraded microbubbles in water showed that degraded products and intact microbubbles could be distinguished, thus it was possible to quantify the intact microbubbles solely. Analysis of human blood plasma spiked with either plain microbubbles or microbubbles with nanoparticles demonstrated that it is possible to separate them from biological components like proteins in these kinds of samples.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2016. Vol. 39, no 8, 1551-1558 p.
Keyword [en]
Capillary electrophoresis, Contrast agents, Human blood plasma, Polyvinyl alcohol microbubbles
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-187204DOI: 10.1002/jssc.201501342ISI: 000374773000018Scopus ID: 2-s2.0-84963701455OAI: oai:DiVA.org:kth-187204DiVA: diva2:929464
Funder
EU, FP7, Seventh Framework Programme, 245572
Note

QC 20160518

Available from: 2016-05-18 Created: 2016-05-18 Last updated: 2017-10-11Bibliographically approved
In thesis
1. Bioanalytical separation using capillary electrophoresis: Applications with microbubbles and proteins
Open this publication in new window or tab >>Bioanalytical separation using capillary electrophoresis: Applications with microbubbles and proteins
2017 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis the possibilities of using capillary electrophoresis as a separation technique for analysis of proteins and microbubbles is presented.

A complete analytical process consists of five necessary steps of which one is the actual analysis step. For this step a suitable analytical technique is needed. Capillary electrophoresis (CE) is one of the common analytical separation techniques used for analysis of a diversity of analytes, and can be both used in routine analysis and for research purposes. The reason for using CE, compared to other liquid-based separation techniques, is mainly short analysis time, high resolution, and negligible sample volumes and solvent waste. Depending on the characteristics of the analytes, and the sample matrix, different modes of CE can be used, where capillary zone electrophoresis (CZE) is the most employed one. The basic principle of CZE is separation of the analytes due to differences in total mobility, which is dependent on the charge and size of the analytes, and the electroosmotic flow (EOF). The EOF can be controlled by several parameters e.g. choice of background electrolyte (BGE), and the optimization of the parameters has been discussed throughout the thesis.

To improve the properties of the BGE, an ethylammonium nitrate (EAN) water solution was used as BGE for CE analysis in Paper I. The precision of the EOF with this method was determined by adjusting the pH of the BGE, the concentration of EAN in the BGE, and the electric field. Model proteins were thereafter analysed using the optimal parameters yielding a precision sufficient for routine control.

One example of the applications of CE is separation of novel contrast agents, which consist of polyvinyl alcohol microbubbles (PVA-MBs). In Paper II, a method for analysis of PVA-MBs in biological samples using CE with UV-detection was developed. It was also established that intact PVA-MBs could be distinguished from ultrasound degraded PVA-MBs in the same set-up.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2017
Series
TRITA-CHE-Report, ISSN 1654-1081 ; 2017:32
Keyword
Background electrolyte, capillary electrophoresis, contrast agents, electroosmotic flow, ethylammonium nitrate, ionic liquids, microbubbles, particles, polyvinyl alcohol microbubbles, and separation
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-215194 (URN)978-91-7729-560-0 (ISBN)
Presentation
2017-11-23, K52, Teknikringen 28, plan 5, Stockholm, 10:00 (English)
Supervisors
Note

QC 20171012

Available from: 2017-10-12 Created: 2017-10-11 Last updated: 2017-10-12Bibliographically approved

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