Analysis of polyvinyl alcohol microbubbles in human blood plasma using capillary electrophoresis
2016 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 39, no 8, 1551-1558 p.Article in journal (Refereed) PublishedText
Recently, a new type of ultrasound contrast agent that consists of air-filled microbubbles stabilized with a shell of polyvinyl alcohol was developed. When superparamagnetic nanoparticles of iron oxide are incorporated in the polymer shell, a multimodal contrast agent can be obtained. The biodistribution and elimination pathways of the polyvinyl alcohol microbubbles are essential to investigate, which is limited with today's techniques. The aim of the present study was, therefore, to develop a method for qualitative and quantitative analysis of microbubbles in biological samples using capillary electrophoresis with ultraviolet detection. The analysis parameters were optimized to a wavelength at 260 nm and pH of the background electrolyte ranging between 11.9 and 12. Studies with high-intensity ultrasonication degraded microbubbles in water showed that degraded products and intact microbubbles could be distinguished, thus it was possible to quantify the intact microbubbles solely. Analysis of human blood plasma spiked with either plain microbubbles or microbubbles with nanoparticles demonstrated that it is possible to separate them from biological components like proteins in these kinds of samples.
Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2016. Vol. 39, no 8, 1551-1558 p.
Capillary electrophoresis, Contrast agents, Human blood plasma, Polyvinyl alcohol microbubbles
IdentifiersURN: urn:nbn:se:kth:diva-187204DOI: 10.1002/jssc.201501342ISI: 000374773000018ScopusID: 2-s2.0-84963701455OAI: oai:DiVA.org:kth-187204DiVA: diva2:929464
FunderEU, FP7, Seventh Framework Programme, 245572
QC 201605182016-05-182016-05-182016-05-31Bibliographically approved