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Analysis of polyvinyl alcohol microbubbles in human blood plasma using capillary electrophoresis
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-2821-1462
KTH, School of Technology and Health (STH), Medical Engineering, Medical Imaging.ORCID iD: 0000-0002-4757-7232
KTH, School of Technology and Health (STH), Medical Engineering, Medical Imaging. Karolinska Institute, Sweden.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-3444-9987
2016 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 39, no 8, 1551-1558 p.Article in journal (Refereed) PublishedText
Abstract [en]

Recently, a new type of ultrasound contrast agent that consists of air-filled microbubbles stabilized with a shell of polyvinyl alcohol was developed. When superparamagnetic nanoparticles of iron oxide are incorporated in the polymer shell, a multimodal contrast agent can be obtained. The biodistribution and elimination pathways of the polyvinyl alcohol microbubbles are essential to investigate, which is limited with today's techniques. The aim of the present study was, therefore, to develop a method for qualitative and quantitative analysis of microbubbles in biological samples using capillary electrophoresis with ultraviolet detection. The analysis parameters were optimized to a wavelength at 260 nm and pH of the background electrolyte ranging between 11.9 and 12. Studies with high-intensity ultrasonication degraded microbubbles in water showed that degraded products and intact microbubbles could be distinguished, thus it was possible to quantify the intact microbubbles solely. Analysis of human blood plasma spiked with either plain microbubbles or microbubbles with nanoparticles demonstrated that it is possible to separate them from biological components like proteins in these kinds of samples.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2016. Vol. 39, no 8, 1551-1558 p.
Keyword [en]
Capillary electrophoresis, Contrast agents, Human blood plasma, Polyvinyl alcohol microbubbles
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-187204DOI: 10.1002/jssc.201501342ISI: 000374773000018ScopusID: 2-s2.0-84963701455OAI: oai:DiVA.org:kth-187204DiVA: diva2:929464
Funder
EU, FP7, Seventh Framework Programme, 245572
Note

QC 20160518

Available from: 2016-05-18 Created: 2016-05-18 Last updated: 2016-05-31Bibliographically approved

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Josefsson, LeilaLarsson, Malin K.Bjällmark, AnnaEmmer, Åsa
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