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Introducing the Affinity Binder Knockdown Initiative-A public-private partnership for validation of affinity reagents
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-2643-8241
KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-7034-0850
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-8993-048X
2016 (English)In: EuPA Open Proteomics, ISSN 0014-2328, E-ISSN 2212-9685, Vol. 10, 56-58 p.Article in journal (Refereed) Published
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Text
Abstract [en]

The newly launched Affinity Binder Knockdown Initiative encourages antibody suppliers and users to join this public-private partnership, which uses crowdsourcing to collect characterization data on antibodies. Researchers are asked to share validation data from experiments where gene-editing techniques (such as siRNA or CRISPR) have been used to verify antibody binding. The initiative is launched under the aegis of Antibodypedia, a database designed to allow comparisons and scoring of publicly available antibodies towards human protein targets. What is known about an antibody is the foundation of the scoring and ranking system in Antibodypedia.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 10, 56-58 p.
Keyword [en]
Antibody quality, CRISPR, Crowdsourcing, Reproducibility, SiRNA, Validation
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-187298DOI: 10.1016/j.euprot.2016.01.002Scopus ID: 2-s2.0-84956679997OAI: oai:DiVA.org:kth-187298DiVA: diva2:929690
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20160519

Available from: 2016-05-19 Created: 2016-05-19 Last updated: 2016-05-19Bibliographically approved

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Alm, ToveLundberg, EmmaUhlén, Mathias
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