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Silk matrices promote formation of insulin-secreting islet-like clusters
KTH, School of Biotechnology (BIO), Protein Technology.
KTH, School of Biotechnology (BIO), Protein Technology.
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2016 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 90, 50-61 p.Article in journal (Refereed) PublishedText
Abstract [en]

Ex vivo expansion of endocrine cells constitutes an interesting alternative to be able to match the unmet need of transplantable pancreatic islets. However, endocrine cells become fragile once removed from their extracellular matrix (ECM) and typically become senescent and loose insulin expression during conventional 2D culture. Herein we develop a protocol where 3D silk matrices functionalized with ECM derived motifs are used for generation of insulin-secreting islet-like clusters from mouse and human primary cells. The obtained clusters were shown to attain an islet-like spheroid shape and to maintain functional insulin release upon glucose stimulation in vitro. Furthermore, in vivo imaging of transplanted murine clusters showed engraftment with increasing vessel formation during time. There was no sign of cell death and the clusters maintained or increased in size throughout the period, thus suggesting a suitable cluster size for transplantation.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 90, 50-61 p.
Keyword [en]
Recombinant spider silk, Pancreatic islets, Islet-like clusters, Insulin release, In vivo imaging, Vascularization
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-187325DOI: 10.1016/j.biomaterials.2016.03.006ISI: 000374618100005PubMedID: 26986856ScopusID: 2-s2.0-84960904715OAI: oai:DiVA.org:kth-187325DiVA: diva2:929913
Note

QC 20160520

Available from: 2016-05-20 Created: 2016-05-20 Last updated: 2016-05-20Bibliographically approved

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Dekki Shalaly, NancyHedhammar, My
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