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The influence of hyaluronan on the structure of a DPPC-bilayer under high pressures
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
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2016 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 142, 230-238 p.Article in journal (Refereed) PublishedText
Abstract [en]

The superior lubrication properties of synovial joints have inspired many studies aiming at uncovering the molecular mechanisms which give rise to low friction and wear. However, the mechanisms are not fully understood yet, and, in particular, it has not been elucidated how the biolubricants present at the interface of cartilage respond to high pressures, which arise during high loads of joints. In this study we utilize a simple model system composed of two biomolecules that have been implied as being important for joint lubrication. It consists of a solid supported dipalmitoylphosphatidylcholin (DPPC) bilayer, which was formed via vesicles fusion on a flat Si wafer, and the anionic polysaccharide hyaluronan (HA). We first characterized the structure of the HA layer that adsorbed to the DPPC bilayers at ambient pressure and different temperatures using X-ray reflectivity (XRR) measurements. Next, XRR was utilized to evaluate the response of the system to high hydrostatic pressures, up to 2 kbar (200 MPa), at three different temperatures. By means of fluorescence microscopy images the distribution of DPPC and HA on the surface was visualized. Our data suggest that HA adsorbs to the headgroup region that is oriented towards the water side of the supported bilayer. Phase transitions of the bilayer in response to temperature and pressure changes were also observed in presence and absence of HA. Our results reveal a higher stability against high hydrostatic pressures for DPPC/HA composite layers compared to that of the DPPC bilayer in absence of HA.

Place, publisher, year, edition, pages
2016. Vol. 142, 230-238 p.
Keyword [en]
Lubrication, Synovial joint, Vesicle fusion, Supported DPPC bilayer, Hyaluronan, High pressure, X-ray reflectivity
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-187770DOI: 10.1016/j.colsurfb.2016.02.040ISI: 000375169600028PubMedID: 26954090OAI: oai:DiVA.org:kth-187770DiVA: diva2:931593
Funder
EU, FP7, Seventh Framework Programme, 312284
Note

QC 20160530

Available from: 2016-05-30 Created: 2016-05-30 Last updated: 2016-05-30Bibliographically approved

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