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The influence of hyaluronan on the structure of a DPPC-bilayer under high pressures
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
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2016 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 142, 230-238 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

The superior lubrication properties of synovial joints have inspired many studies aiming at uncovering the molecular mechanisms which give rise to low friction and wear. However, the mechanisms are not fully understood yet, and, in particular, it has not been elucidated how the biolubricants present at the interface of cartilage respond to high pressures, which arise during high loads of joints. In this study we utilize a simple model system composed of two biomolecules that have been implied as being important for joint lubrication. It consists of a solid supported dipalmitoylphosphatidylcholin (DPPC) bilayer, which was formed via vesicles fusion on a flat Si wafer, and the anionic polysaccharide hyaluronan (HA). We first characterized the structure of the HA layer that adsorbed to the DPPC bilayers at ambient pressure and different temperatures using X-ray reflectivity (XRR) measurements. Next, XRR was utilized to evaluate the response of the system to high hydrostatic pressures, up to 2 kbar (200 MPa), at three different temperatures. By means of fluorescence microscopy images the distribution of DPPC and HA on the surface was visualized. Our data suggest that HA adsorbs to the headgroup region that is oriented towards the water side of the supported bilayer. Phase transitions of the bilayer in response to temperature and pressure changes were also observed in presence and absence of HA. Our results reveal a higher stability against high hydrostatic pressures for DPPC/HA composite layers compared to that of the DPPC bilayer in absence of HA.

Place, publisher, year, edition, pages
2016. Vol. 142, 230-238 p.
Keyword [en]
Lubrication, Synovial joint, Vesicle fusion, Supported DPPC bilayer, Hyaluronan, High pressure, X-ray reflectivity
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-187770DOI: 10.1016/j.colsurfb.2016.02.040ISI: 000375169600028PubMedID: 26954090OAI: oai:DiVA.org:kth-187770DiVA: diva2:931593
Funder
EU, FP7, Seventh Framework Programme, 312284
Note

QC 20160530

Available from: 2016-05-30 Created: 2016-05-30 Last updated: 2017-02-09Bibliographically approved
In thesis
1. Synergies in Biolubrication
Open this publication in new window or tab >>Synergies in Biolubrication
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The objective of this thesis was to advance understanding in the field of biolubrication, finding inspiration from the human synovial joints. This was addressed by investigating the association of key biolubricants and the resulting lubrication performance. Techniques employed during the course of this work were Atomic force microscopy (AFM), Quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray reflectivity (XRR).

Key synovial fluid and cartilage components like dipalmitoylphosphatidylcholine (DPPC), hyaluronan (HA), lubricin, and cartilage oligomeric matrix protein (COMP) have been used in the investigations. Focus was towards two lubrication couples; DPPC-hyaluronan and COMP-lubricin. DPPC-hyaluronan mixtures were probed on hydrophilic silica surfaces and COMP-lubricin association structures were explored on weakly hydrophobic poly (methyl methacrylate) (PMMA) surfaces.

Investigations of the COMP-lubricin pair revealed that individually these components are unable to reach desired lubrication. However in combination, COMP facilitates firm attachment of lubricin to the PMMA surface in a favourable confirmation that imparts low friction coefficient.

DPPC and hyaluronan combined impart lubrication advantage over lone DPPC bilayers. Hyaluronan provides a reservoir of DPPC on the surface and consequently self-healing ability.

Other factors like temperature, presence of calcium ions, molecular weight of hyaluronan, and pressure were also explored. DPPC bilayers at higher temperature had higher load bearing capacity. Association between DPPC Langmuir layers and hyaluronan was enhanced in the presence of calcium ions, and lower molecular weight hyaluronan had a stronger tendency to bind to DPPC. At high pressures, DPPC-hyaluronan layers were more stable compared to lone DPPC bilayers.

Place, publisher, year, edition, pages
Sweden: KTH Royal Institute of Technology, 2017. 66 p.
Series
TRITA-CHE-Report, ISSN 1654-1081
Keyword
Biolubrication, Synergies, Adsorption, Surface Force, Friction, Load Bearing Capacity, Self Healing, Phospholipids, DPPC, Hyaluronan, COMP, Lubricin, QCM-D, AFM, XRR.
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-201219 (URN)978-91-7729-268-5 (ISBN)
Public defence
2017-03-17, Kollegiesalen, KTH, Brinellvägen 8, Stockholm, 10:00 (English)
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Supervisors
Note

QC 20170210

Available from: 2017-02-10 Created: 2017-02-09 Last updated: 2017-02-13Bibliographically approved

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