A saposin-lipoprotein nanoparticle system for membrane proteins
2016 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 13, no 4, 345-351 p.Article in journal (Refereed) PublishedText
A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrate the applicability of the method on two purified membrane protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral membrane protein complex from the virus membrane. Our approach facilitated high-resolution structural studies of the bacterial peptide transporter PeptT(S02) by single-particle cryo-electron microscopy (cryo-EM) and allowed us to stabilize the HIV envelope glycoprotein in a functional state.
Place, publisher, year, edition, pages
Nature Publishing Group, 2016. Vol. 13, no 4, 345-351 p.
IdentifiersURN: urn:nbn:se:kth:diva-186643DOI: 10.1038/NMETH.3801ISI: 000374084800023PubMedID: 26950744ScopusID: 2-s2.0-84960194524OAI: oai:DiVA.org:kth-186643DiVA: diva2:932429
QC 201606012016-06-012016-05-132016-06-01Bibliographically approved