Change search
ReferencesLink to record
Permanent link

Direct link
Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories
Show others and affiliations
2016 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 7, 11709Article in journal (Refereed) PublishedText
Abstract [en]

Sustainable production of oleochemicals requires establishment of cell factory platform strains. The yeast Saccharomyces cerevisiae is an attractive cell factory as new strains can be rapidly implemented into existing infrastructures such as bioethanol production plants. Here we show high-level production of free fatty acids (FFAs) in a yeast cell factory, and the production of alkanes and fatty alcohols from its descendants. The engineered strain produces up to 10.4 gl(-1) of FFAs, which is the highest reported titre to date. Furthermore, through screening of specific pathway enzymes, endogenous alcohol dehydrogenases and aldehyde reductases, we reconstruct efficient pathways for conversion of fatty acids to alkanes (0.8 mgl(-1)) and fatty alcohols (1.5 gl(-1)), to our knowledge the highest titres reported in S. cerevisiae. This should facilitate the construction of yeast cell factories for production of fatty acids derived products and even aldehyde-derived chemicals of high value.

Place, publisher, year, edition, pages
Nature Publishing Group, 2016. Vol. 7, 11709
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-188740DOI: 10.1038/ncomms11709ISI: 000376671300001PubMedID: 27222209OAI: diva2:939656

QC 20160620

Available from: 2016-06-20 Created: 2016-06-17 Last updated: 2016-06-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nielsen, Jens
By organisation
Gene Technology
In the same journal
Nature Communications
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 3 hits
ReferencesLink to record
Permanent link

Direct link