Change search
ReferencesLink to record
Permanent link

Direct link
Molecular Architecture of Yeast Chromatin Assembly Factor 1
KTH, School of Technology and Health (STH).
Show others and affiliations
2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 26702Article in journal (Refereed) PublishedText
Abstract [en]

Chromatin Assembly Complex 1 (CAF-1) is a major histone chaperone involved in deposition of histone H3 and H4 into nucleosome. CAF-1 is composed of three subunits; p150, p60 and p48 for human and Cac1, Cac2 and Cac3 for yeast. Despite of its central role in chromatin formation, structural features of the full CAF-1 in complex with histones and other chaperones have not been well characterized. Here, we dissect molecular architecture of yeast CAF-1 (yCAF-1) by cross-linking mass spectrometry (XL-MS) and negative stain single-particle electron microscopy (EM). Our work revealed that Cac1, the largest subunit of yCAF-1, might serve as a major histone binding platform linking Cac2 and Cac3. In addition, EM analysis showed that yCAF-1 adopts a bilobal shape and Cac1 connecting Cac2 and Cac3 to generate a platform for binding histones. This study provides the first structural glimpse of the full CAF-1 complex and a structural framework to understand histone chaperoning processes.

Place, publisher, year, edition, pages
Nature Publishing Group, 2016. Vol. 6, 26702
URN: urn:nbn:se:kth:diva-188727DOI: 10.1038/srep26702ISI: 000376499900001PubMedID: 27221973ScopusID: 2-s2.0-84971317627OAI: diva2:939734

QC 20160620

Available from: 2016-06-20 Created: 2016-06-17 Last updated: 2016-06-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus
By organisation
School of Technology and Health (STH)
In the same journal
Scientific Reports

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 2 hits
ReferencesLink to record
Permanent link

Direct link