Monitoring Nanostructure Dynamics and Polymerization in Glycerol Plasticized Wheat Gliadin and Glutenin Films: Relation to Mechanical Properties
2016 (English)In: ACS SUSTAINABLE CHEMISTRY & ENGINEERING, ISSN 2168-0485, Vol. 4, no 6, 2998-3007 p.Article in journal (Refereed) PublishedText
Gliadin and glutenin proteins with 10, 20, 30 and 40% of glycerol were compression molded into films (130 degrees C) and evaluated for protein polymerization, beta-sheet structure and nano-structural morphology. Here, for the first time we show how different amounts of glycerol impact the nano-structure and functional properties of the gliadin and glutenin films. Most polymerized protein was found in the gliadin films with 20 and 30% glycerol, and in all the glutenin films (except 10%), by RP-HPLC. A beta-sheet-rich protein structure was found to be high in the 10 and 20% glycerol gliadin films, and in the 20 and 30% glycerol glutenin films by FT-IR. Glycerol content of 20, 30 and 40% impacted the nano-structural morphology of the gliadin glycerol films observed by SAXS, and to a limited extent for 10 and 20% glycerol gliadin films revealed by WAXS. No ordered nano-structure was found for the glutenin glycerol films. The 20%, 30% and 40% glycerol films were the most tunable for specific mechanical properties. For the highest stiffness and strength, the 10% glycerol protein films were the best choice.
Place, publisher, year, edition, pages
American Chemical Society , 2016. Vol. 4, no 6, 2998-3007 p.
Protein morphology, Protein film, Glycerol content, Polymerization, SAXS, WAXS
IdentifiersURN: urn:nbn:se:kth:diva-189377DOI: 10.1021/acssuschemeng.5b01667ISI: 000377425900011ScopusID: 2-s2.0-84973539342OAI: oai:DiVA.org:kth-189377DiVA: diva2:947283
QC 201607072016-07-072016-07-042016-07-07Bibliographically approved