A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds
2016 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468Article in journal, Letter (Refereed) Epub ahead of print
The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and 31P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.
Place, publisher, year, edition, pages
31P NMR; carbohydrate esterase; lignin-carbohydrate complexes; size exclusion chromatography; spruce and birch
Wood Science Microbiology
IdentifiersURN: urn:nbn:se:kth:diva-190078DOI: 10.1002/1873-3468.12290OAI: oai:DiVA.org:kth-190078DiVA: diva2:950997
FunderKnut and Alice Wallenberg Foundation, 8107
J. Arnling Bååth and N. Giummarella contributed equally to this work.
QC 201608082016-08-042016-08-042016-08-08Bibliographically approved