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A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds
Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology, Wood Chemistry and Pulp Technology. KTH, School of Chemical Science and Engineering (CHE), Centres, Wallenberg Wood Science Center.ORCID iD: 0000-0003-4266-0720
Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology, Wood Chemistry and Pulp Technology. KTH, School of Chemical Science and Engineering (CHE), Centres, Wallenberg Wood Science Center.
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2016 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468Article in journal, Letter (Refereed) Epub ahead of print
Abstract [en]

The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and 31P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.

Place, publisher, year, edition, pages
2016.
Keyword [en]
31P NMR; carbohydrate esterase; lignin-carbohydrate complexes; size exclusion chromatography; spruce and birch
National Category
Wood Science Microbiology
Identifiers
URN: urn:nbn:se:kth:diva-190078DOI: 10.1002/1873-3468.12290OAI: oai:DiVA.org:kth-190078DiVA: diva2:950997
Funder
Knut and Alice Wallenberg Foundation, 8107
Note

J. Arnling Bååth and N. Giummarella contributed equally to this work.

QC 20160808

Available from: 2016-08-04 Created: 2016-08-04 Last updated: 2016-08-08Bibliographically approved

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Publisher's full texthttp://onlinelibrary.wiley.com/doi/10.1002/1873-3468.12290/full

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