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Bioelectric and Morphological Response of Liquid-Covered Human Airway Epithelial Calu-3 Cell Monolayer to Periodic Deposition of Colloidal 3-Mercaptopropionic-Acid Coated CdSe-CdS/ZnS Core-Multishell Quantum Dots
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
AstraZeneca R and D; Sweden.
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 2, e0149915Article in journal (Refereed) Published
Abstract [en]

Lung epithelial cells are extensively exposed to nanoparticles present in the modern urban environment. Nanoparticles, including colloidal quantum dots (QDs), are also considered to be potentially useful carriers for the delivery of drugs into the body. It is therefore important to understand the ways of distribution and the effects of the various types of nanoparticles in the lung epithelium. We use a model system of liquid-covered human airway epithelial Calu-3 cell cultures to study the immediate and long-term effects of repeated deposition of colloidal 3-mercaptopropionic-acid coated CdSe-CdS/ZnS core-multishell QDs on the lung epithelial cell surface. By live confocal microscope imaging and by QD fluorescence measurements we show that the QD permeation through the mature epithelial monolayers is very limited. At the time of QD deposition, the transepithelial electrical resistance (TEER) of the epithelial monolayers transiently decreased, with the decrement being proportional to the QD dose. Repeated QD deposition, once every six days for two months, lead to accumulation of only small amounts of the QDs in the cell monolayer. However, it did not induce any noticeable changes in the long-term TEER and the molecular morphology of the cells. The colloidal 3-mercaptopropionic-acid coated CdSe-CdS/ZnS core-multishell QDs could therefore be potentially used for the delivery of drugs intended for the surface of the lung epithelia during limited treatment periods. © 2016 Turdalieva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Place, publisher, year, edition, pages
Public Library of Science , 2016. Vol. 11, no 2, e0149915
Keyword [en]
Tight Junctions, Primary Cilia, Lung-Cells, In-Vitro, Nanoparticles, Toxicity, Promotes, Mucus, Line
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:kth:diva-191847DOI: 10.1371/journal.pone.0149915ISI: 000371175700035PubMedID: 26913754ScopusID: 2-s2.0-84977674241OAI: oai:DiVA.org:kth-191847DiVA: diva2:957605
Funder
Swedish Research Council, 621-2011-4381Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20160927 QC 20160927

Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2016-09-27Bibliographically approved
In thesis
1. Fluorescence Properties of Quantum Dots and Their Utilization in Bioimaging
Open this publication in new window or tab >>Fluorescence Properties of Quantum Dots and Their Utilization in Bioimaging
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Quantum dots (QDs), especially colloidal semiconductor QDs, possess properties including high quantum yields, narrow fluorescence spectra, broad absorption and excellent photostability, making them extremely powerful in bioimaging. In this thesis, we studied the fluorescence properties of QDs and attempted multiple ways to boost applications of QDs in bioimaging field.

By time-correlated single photon counting (TCSPC) measurement, we quantitatively interpreted the fluorescence mechanism of colloidal semiconductor QDs.

To enhance QD fluorescence, we used a porous alumina membrane as a photonic crystal structure to modulate QD fluorescence.

We studied the acid dissociation of 3-mercaptopropionic acid (MPA) coated QDs mainly through electrophoretic mobility of 3-MPA coated CdSe QDs and successfully demonstrated the impact of pH change and Ca2+ ions.

Blinking phenomena of both CdSe-CdS/ZnS core-shell QDs and 3C-SiC nanocrystals (NCs) were studied. A general model on blinking characteristics relates the on-state distribution to CdSe QD surface conditions. The energy relaxation pathway of fluorescence of 3C-SiC NCs was found independent of surface states.

To examine QD effect on ciliated cells, we conducted a 70-day long experiment on the bioelectric and morphological response of human airway epithelial Calu-3 cells with periodic deposition of 3-MPA coated QDs and found the cytotoxicity of QDs was found very low.

In a brief summary, our study of QD could benefit in bioimaging and biosensing. Especially, super-resolution fluorescent bioimaging, such as, stochastic optical reconstruction microscopy (STORM) and photo-activated localization microscopy (PALM), may benefit from the modulation of the QD blinking in this study. And fluorescence lifetime imaging (FLIM) microscopy could take advantage of lifetime modulation based on our QD lifetime study.

Place, publisher, year, edition, pages
KTH Royal Institute of Technology, 2016. 77 p.
Series
TRITA-FYS, ISSN 0280-316X ; 2016:54
Keyword
Fluorescence, Microscopy, Bioimaging, Nanomaterial, cytotoxicity, mechanism
National Category
Biophysics
Research subject
Biological Physics
Identifiers
urn:nbn:se:kth:diva-191985 (URN)78-91-7729-074-2 (ISBN)
External cooperation:
Public defence
2016-09-29, Seminar room Earth, Science for life laboratory, Tomtebodavägen 23A, Solna, 13:00 (English)
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Supervisors
Note

QC 20160905

Available from: 2016-09-07 Created: 2016-09-02 Last updated: 2016-09-07Bibliographically approved

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