Droplet size influences division of mammalian cell factories in droplet microfluidic cultivation
2016 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683Article in journal (Refereed) Epub ahead of print
The potential of using droplet microfluidics for screening mammalian cell factories has been limited by the difficulty in achieving continuous cell division during cultivation in droplets. Here, we report the influence of droplet size on mammalian cell division and viability during cultivation in droplets. Chinese Hamster Ovary (CHO) cells, the most widely used mammalian host cells for biopharmaceuticals production were encapsulated and cultivated in 33, 180 and 320 pL droplets for 3 days. Periodic monitoring of the droplets during incubation showed that the cell divisions in 33 pL droplets stopped after 24 h, whereas continuous cell division was observed in 180 and 320 pL droplets for 72 h. The viability of the cells cultivated in the 33 pL droplets also dropped to about 50% in 72 h. In contrast, the viability of the cells in the larger droplets was above 90% even after 72 h of cultivation, making them a more suitable droplet size for 72-h cultivation. This study shows a direct correlation of microfluidic droplet size to the division and viability of mammalian cells. This highlights the importance of selecting suitable droplet size for mammalian cell factory screening assays.
Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2016.
Research subject Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-192574DOI: 10.1002/elps.201600316OAI: oai:DiVA.org:kth-192574DiVA: diva2:971033
QC 201609212016-09-152016-09-152016-09-21Bibliographically approved