Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips
2016 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 18, no 4, 71Article in journal (Refereed) Published
We demonstrate an acoustic platform for microvortexing in disposable polymer microfluidic chips with small-volume (20 mu l) reaction chambers. The described method is demonstrated for a variety of standard vortexing functions, including mixing of fluids, re-suspension of a pellet of magnetic beads collected by a magnet placed on the chip, and lysis of cells for DNA extraction. The device is based on a modified Langevin-type ultrasonic transducer with an exponential horn for efficient coupling into the microfluidic chip, which is actuated by a low-cost fixed-frequency electronic driver board. The transducer is optimized by numerical modelling, and different demonstrated vortexing functions are realized by actuating the transducer for varying times; from fractions of a second for fluid mixing, to half a minute for cell lysis and DNA extraction. The platform can be operated during 1 min below physiological temperatures with the help of a PC fan, a Peltier element and an aluminum heat sink acting as the chip holder. As a proof of principle for sample preparation applications, we demonstrate on-chip cell lysis and DNA extraction within 25 s. The method is of interest for automating and chip-integrating sample preparation procedures in various biological assays.
Place, publisher, year, edition, pages
Springer, 2016. Vol. 18, no 4, 71
Acoustic streaming, Lab on a chip, Cell lysis
IdentifiersURN: urn:nbn:se:kth:diva-192740DOI: 10.1007/s10544-016-0097-4ISI: 000381117500019PubMedID: 27444649ScopusID: 2-s2.0-84979256248OAI: oai:DiVA.org:kth-192740DiVA: diva2:974389
QC 201609262016-09-262016-09-202016-09-26Bibliographically approved