Change search
ReferencesLink to record
Permanent link

Direct link
Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences (SCI), Applied Physics.
Show others and affiliations
2016 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 18, no 4, 71Article in journal (Refereed) Published
Abstract [en]

We demonstrate an acoustic platform for microvortexing in disposable polymer microfluidic chips with small-volume (20 mu l) reaction chambers. The described method is demonstrated for a variety of standard vortexing functions, including mixing of fluids, re-suspension of a pellet of magnetic beads collected by a magnet placed on the chip, and lysis of cells for DNA extraction. The device is based on a modified Langevin-type ultrasonic transducer with an exponential horn for efficient coupling into the microfluidic chip, which is actuated by a low-cost fixed-frequency electronic driver board. The transducer is optimized by numerical modelling, and different demonstrated vortexing functions are realized by actuating the transducer for varying times; from fractions of a second for fluid mixing, to half a minute for cell lysis and DNA extraction. The platform can be operated during 1 min below physiological temperatures with the help of a PC fan, a Peltier element and an aluminum heat sink acting as the chip holder. As a proof of principle for sample preparation applications, we demonstrate on-chip cell lysis and DNA extraction within 25 s. The method is of interest for automating and chip-integrating sample preparation procedures in various biological assays.

Place, publisher, year, edition, pages
Springer, 2016. Vol. 18, no 4, 71
Keyword [en]
Acoustic streaming, Lab on a chip, Cell lysis
National Category
Nano Technology
URN: urn:nbn:se:kth:diva-192740DOI: 10.1007/s10544-016-0097-4ISI: 000381117500019PubMedID: 27444649ScopusID: 2-s2.0-84979256248OAI: diva2:974389

QC 20160926

Available from: 2016-09-26 Created: 2016-09-20 Last updated: 2016-09-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Iranmanesh, IdaOhlin, MathiasRamachandraiah, HarishaYe, SimonRussom, AmanWiklund, Martin
By organisation
Applied PhysicsProteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLabBiomedical and X-ray Physics
In the same journal
Biomedical microdevices (Print)
Nano Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

ReferencesLink to record
Permanent link

Direct link