Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0003-0064-0086
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-7023-4772
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5199-0663
KTH, School of Engineering Sciences (SCI), Applied Physics.
Show others and affiliations
2016 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 18, no 4, 71Article in journal (Refereed) Published
Abstract [en]

We demonstrate an acoustic platform for microvortexing in disposable polymer microfluidic chips with small-volume (20 mu l) reaction chambers. The described method is demonstrated for a variety of standard vortexing functions, including mixing of fluids, re-suspension of a pellet of magnetic beads collected by a magnet placed on the chip, and lysis of cells for DNA extraction. The device is based on a modified Langevin-type ultrasonic transducer with an exponential horn for efficient coupling into the microfluidic chip, which is actuated by a low-cost fixed-frequency electronic driver board. The transducer is optimized by numerical modelling, and different demonstrated vortexing functions are realized by actuating the transducer for varying times; from fractions of a second for fluid mixing, to half a minute for cell lysis and DNA extraction. The platform can be operated during 1 min below physiological temperatures with the help of a PC fan, a Peltier element and an aluminum heat sink acting as the chip holder. As a proof of principle for sample preparation applications, we demonstrate on-chip cell lysis and DNA extraction within 25 s. The method is of interest for automating and chip-integrating sample preparation procedures in various biological assays.

Place, publisher, year, edition, pages
Springer, 2016. Vol. 18, no 4, 71
Keyword [en]
Acoustic streaming, Lab on a chip, Cell lysis
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:kth:diva-192740DOI: 10.1007/s10544-016-0097-4ISI: 000381117500019PubMedID: 27444649Scopus ID: 2-s2.0-84979256248OAI: oai:DiVA.org:kth-192740DiVA: diva2:974389
Note

QC 20160926

Available from: 2016-09-26 Created: 2016-09-20 Last updated: 2017-11-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Iranmanesh, IdaOhlin, MathiasRamachandraiah, HarishaYe, SimonRussom, AmanWiklund, Martin
By organisation
Applied PhysicsProteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLabBiomedical and X-ray Physics
In the same journal
Biomedical microdevices (Print)
Nano Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 5 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf