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  • 1.
    Chinnasamy, Thiruppathiraja
    et al.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Segerink, Loes I.
    Nystrand, Mats
    Gantelius, Jesper
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Andersson Svahn, Helene
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    A lateral flow paper microarray for rapid allergy point of care diagnostics2014In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 139, no 10, p. 2348-2354Article in journal (Refereed)
    Abstract [en]

    There is a growing need for multiplexed specific IgE tests that can accurately evaluate patient sensitization profiles. However, currently available commercial tests are either single/low-plexed or require sophisticated instrumentation at considerable cost per assay. Here, we present a novel convenient lateral flow microarray-based device that employs a novel dual labelled gold nanoparticle-strategy for rapid and sensitive detection of a panel of 15 specific IgE responses in 35 clinical serum samples. Each gold nanoparticle was conjugated to an optimized ratio of HRP and anti-IgE, allowing significant enzymatic amplification to improve the sensitivity of the assay as compared to commercially available detection reagents. The mean inter-assay variability of the developed LFM assay was 12% CV, and analysis of a cohort of clinical samples (n = 35) revealed good general agreement with ImmunoCAP, yet with a varying performance among allergens (AUC = [0.54-0.88], threshold 1 kU). Due to the rapid and simple procedure, inexpensive materials and read-out by means of a consumer flatbed scanner, the presented assay may provide an interesting low-cost alternative to existing multiplexed methods when thresholds > 1 kU are acceptable.

  • 2. Crespo, Gaston A.
    et al.
    Bakker, Eric
    Ionophore-based ion optodes without a reference ion: electrogenerated chemiluminescence for potentiometric sensors2012In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 137, no 21, p. 4988-4994Article in journal (Refereed)
  • 3. Dorokhin, Denis
    et al.
    Crespo, Gaston A.
    Afshar, Majid Ghahraman
    Bakker, Eric
    A low-cost thin layer coulometric microfluidic device based on an ion-selective membrane for calcium determination2014In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 139, no 1, p. 48-51Article in journal (Refereed)
  • 4.
    Duner, Gunnar
    et al.
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
    Anderson, Henrik
    Pei, Zhichao
    Ingemarsson, Bjorn
    Aastrup, Teodor
    Ramstrom, Olof
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Signal enhancement in ligand-receptor interactions using dynamic polymers at quartz crystal microbalance sensors2016In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 141, no 13, p. 3993-3996Article in journal (Refereed)
    Abstract [en]

    The signal enhancement properties of QCM sensors based on dynamic, biotinylated poly(acrylic acid) brushes has been studied in interaction studies with an anti-biotin Fab fragment. The poly (acrylic acid) sensors showed a dramatic increase in signal response with more than ten times higher signal than the carboxyl-terminated self-assembled monolayer surface.

  • 5. Guinovart, Tomas
    et al.
    Parrilla, Marc
    Crespo, Gaston A.
    Rius, F. Xavier
    Andrade, Francisco J.
    Potentiometric sensors using cotton yarns, carbon nanotubes and polymeric membranes2013In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 138, no 18, p. 5208-5215Article in journal (Refereed)
    Abstract [en]

    A simple and generalized approach to build electrochemical sensors for wearable devices is presented. Commercial cotton yarns are first turned into electrical conductors through a simple dyeing process using a carbon nanotube ink. These conductive yarns are then partially coated with a suitable polymeric membrane to build ion-selective electrodes. Potentiometric measurements using these yarn-potentiometric sensors are demonstrated. Examples of yarns that can sense pH, K+ and NH4+ are presented. In all cases, these sensing yarns show limits of detection and linear ranges that are similar to those obtained with lab-made solid-state ion-selective electrodes. Through the immobilization of these sensors in a band-aid, it is shown that this approach could be easily implemented in a wearable device. Factors affecting the performance of the sensors and future potential applications are discussed.

  • 6. Li, C. Y.
    et al.
    Zhang, X. B.
    Han, Z. X.
    Akermark, B.
    Sun, Licheng C.
    Shen, G. L.
    Yu, R. Q.
    A wide pH range optical sensing system based on a sol-gel encapsulated amino-functionalised corrole2006In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 131, no 3, p. 388-393Article in journal (Refereed)
    Abstract [en]

    The synthesis of a new compound, 10-(4-aminophenyl)-5,15-dimesitylcorrole, and its application for the preparation of optical chemical pH sensors is described. The dye materials were immobilized in a sol - gel glass matrix and characterised upon exposure to aqueous buffer solutions. The response of the sensor is based on the fluorescence intensity changing of corrole owing to multiple steps of protonation and deprotonation. Due to its containing several proton sensitive centers, the 10-(4-aminophenyl)- 5,15-dimesitylcorrole based optode shows a wider response range toward pH than that of tetraphenylporphyrin (TPPH2) and 5,10,15-tris( pentafluorophenyl) corrole (H-3(tpfc)). It shows a linear pH response in the range of 2.17 - 10.30. The effect of the composition of the sensor membrane has been studied and the experimental conditions were optimized. The optode showed good reproducibility and reversibility, and common co-existing inorganic ions did not show obvious interference to its pH measurement.

  • 7. Liu, Wei
    et al.
    Chen, Yi
    Yan, Mingdi
    Portland State University.
    Surface plasmon resonance imaging of limited glycoprotein samples2008In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 133, no 9, p. 1268-1273Article in journal (Refereed)
    Abstract [en]

    A surface plasmon resonance imaging method has been developed for high throughput recognition and determination of low level glycoproteins with limited sample volume at least down to 50 nL. Chicken ovalbumin and immunoglobulin G were chosen as model compounds while bovine serum albumin and lysozyme were used as control. Each protein, at a concentration of 0.0080-1.0 mg mL(-1), was printed on one gold sensing film, and the films were simultaneously reacted with a probe solution and viewed using a laboratory-built surface plasmon resonance imaging system. The imaging signals were dependent on the concentration and the type of analyte, with a limit of detection down to at least 0.5 ng. The glycoproteins dotted at either 1.0 mg mL(-1) or 0.010 mg mL(-1) were easily differentiated from the non-glycoproteins by reaction with 200 nM concanavalin A (con A), giving a limit of recognition down also to 0.5 ng glycoprotein. This imaging method was hence considered a new tool for analyzing glycoproteins.

  • 8. Parra, Enrique J.
    et al.
    Crespo, Gaston A.
    Riu, Jordi
    Ruiz, Aurora
    Rius, F. Xavier
    Ion-selective electrodes using multi-walled carbon nanotubes as ion-to-electron transducers for the detection of perchlorate2009In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 134, no 9, p. 1905-1910Article in journal (Refereed)
  • 9.
    Reuterswärd, Philippa
    et al.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    Gantelius, Jesper
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    Andersson Svahn, Helene
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    An 8 minute colorimetric paper-based reverse phase vertical flow serum microarray for screening of hyper IgE syndrome2015In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 140, no 21, p. 7327-7334Article in journal (Refereed)
    Abstract [en]

    Reverse phase microarrays are useful tools for affinity-based detection in hundreds of samples simultaneously. However, current methods typically require long assay times and fluorescent detection. Here we describe a paper-based Vertical Flow Microarray (VFM) assay as a rapid 8-minute colorimetric alternative for reverse phase microarray analysis. The VFM platform was optimized for detection of IgE with a detection limit of 1.9 μg mL-1 in whole serum. Optimized conditions were then used to screen 113 serum samples simultaneously for hyper IgE syndrome (hIgE), a rare primary immunodeficiency characterized by elevated levels of IgE. The same set of samples were then analysed with a conventional planar microarray with fluorescent detection for head-to-head testing. Both assays found elevated levels in three out of four hIgE patient samples, whereas no control samples displayed elevated levels in either method. The comparison experiments showed a good correlation between the two assays, as determined from a linear correlation study (Pearson's r = 0.76). Further, the assay-time reduction and reproducibility (intra assay CV = 12.4 ± 4.11%) demonstrate the applicability of the VFM platform for high throughput reverse phase screening.

  • 10. Shi, Leilei
    et al.
    Li, Xin
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
    Zhou, Min
    Muhammad, Faheem
    Ding, Yubin
    Wei, Hui
    An arylboronate locked fluorescent probe for hypochlorite2017In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 142, no 12, p. 2104-2108Article in journal (Refereed)
    Abstract [en]

    An unusual arylboronate based fluorescent probe R1 was synthesized for the selective and sensitive detection of ClO-. A detailed mechanistic study revealed that R1 reacted with ClO- through an oxidation to chlorination mechanism, and the arylboronate moiety in R1 acted as a "lock" to eliminate the effects of pH fluctuations. With this design strategy, R1 was successfully used to detect as low as 6.4 nM of ClO- over other ROS species in a wide pH range from 4.5 to 9.0.

  • 11. Wang, Xin
    et al.
    Ramström, Olof
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Yan, Mingdi
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Dynamic light scattering as an efficient tool to study glyconanoparticle-lectin interactions2011In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 136, no 20, p. 4174-4178Article in journal (Refereed)
    Abstract [en]

    Glyconanomaterials, an emerging class of bio-functional nanomaterials, have shown promise in detecting, imaging and targeting proteins, bacteria, and cells. In this article, we report that dynamic light scattering (DLS) can be used as an efficient tool to study glyconanoparticle (GNP)-lectin interactions. Silica and Au nanoparticles (NPs) conjugated with D-mannose (Man) and D-galactose (Gal) were treated with the lectins Concanavalin A (Con A) and Ricinus communis agglutinin (RCA(120)), and the hydrodynamic volumes of the resulting aggregates were measured by DLS. The results showed that the particle size grew with increasing lectin concentration. The limit of detection (LOD) was determined to be 2.9 nM for Con A with Man-conjugated and 6.6 nM for RCA(120) with Gal-conjugated silica NPs (35 nm), respectively. The binding affinity was also determined by DLS and the results showed 3-4 orders of magnitude higher affinity of GNPs than the free ligands with lectins. The assay sensitivity and affinity were particle size dependent and decreased with increasing particle diameter. Because the method relies on the particle size growth, it is therefore general and can be applied to nanomaterials of different compositions.

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