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  • 1.
    Grönkvist, Mikael
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Environmental Physiology.
    LaPelusa, M.
    Univ Texas Rio Grande Valley, Sch Med, Edinburg, TX USA..
    Gennser, Mikael
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Environmental Physiology. Royal Inst Technol, Environm Physiol, Stockholm, Sweden..
    Machado-Moreira, Christiano Antonio
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Environmental Physiology.
    Postural and Daily Variations in the Single-Breath Diffusion Capacity of the Lungs for Carbon Monoxide2018In: American Journal of Respiratory and Critical Care Medicine, ISSN 1073-449X, E-ISSN 1535-4970, Vol. 197Article in journal (Other academic)
  • 2.
    Häggmark, Anna
    et al.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Hamsten, Carl
    Wiklundh, Emil
    Lindskog, Cecilia
    Mattsson, Cecilia
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    Andersson, Eni
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Lundberg, Ingrid E.
    Gronlund, Hans
    Schwenk, Jochen M.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Eklund, Anders
    Grunewald, Johan
    Nilsson, Peter
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Proteomic Profiling Reveals Autoimmune Targets in Sarcoidosis2015In: American Journal of Respiratory and Critical Care Medicine, ISSN 1073-449X, E-ISSN 1535-4970, Vol. 191, no 5, p. 574-583Article in journal (Refereed)
    Abstract [en]

    Rationale: There is a need to further characterize the antibody repertoire in relation to sarcoidosis and potentially related autoantigens. Objectives: We investigated bronchoalveolar lavage (BAL) and serum samples from patients with sarcoidosis and healthy and diseased control subjects to discover sarcoidosis-associated autoantigens. Methods: Antigen microarrays built on 3,072 protein fragments were used to screen for IgG reactivity in 73 BAL samples from subjects with sarcoidosis, subjects with asthma, and healthy subjects. A set of 131 targets were selected for subsequent verification on suspension bead arrays using 272 additional BAL samples and 141 paired sera. Reactivity to four antigens was furthermore analyzed in 22 unprocessed BAL samples from patients with fibrosis and 269 plasma samples from patients diagnosed with myositis. Measurements and Main Results: Reactivity toward zinc finger protein 688 and mitochondrial ribosomal protein L43 were discovered with higher frequencies in patients with sarcoidosis, for mitochondrial ribosomal protein L43 especially in patients with non-Lofgren syndrome. Increased reactivity toward nuclear receptor coactivator 2 was also observed in patients with non-Lofgren syndrome as compared with patients with Lofgren syndrome. The antigen representing adenosine diphosphate-ribosylation factor GTPase activating protein 1 revealed high reactivity frequency in all sample groups but with significantly higher level of IgG reactivities in patients with sarcoidosis. Conclusions: Autoantigen reactivity was present in most BAL and serum samples analyzed, and the results revealed high interindividual heterogeneity, with most of the reactivities observed in single individuals only. Four proteins are here proposed as sarcoidosis-associated autoimmune targets and of interest for further validation in independent cohorts.

  • 3.
    Mikus, Maria
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science.
    Kolmert, J.
    Karolinska Inst, Stockholm, Sweden..
    Andersson, L. I.
    Karolinska Inst, Stockholm, Sweden..
    James, A.
    Karolinska Inst, Stockholm, Sweden..
    Gomez, C.
    Karolinska Inst, Stockholm, Sweden..
    Dahlen, B. U.
    Karolinska Inst, Stockholm, Sweden..
    De Meulder, B.
    European Inst Syst Biol & Med, Lyon, France..
    Djukanovic, R.
    Univ Southampton, Southampton, Hants, England..
    Sterk, P. J.
    Amsterdam Med Univ, Amsterdam, Netherlands..
    Adcock, I. M.
    Imperial Coll, London, England..
    Nilsson, Peter
    KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Dahlen, S. K.
    Karolinska Inst, Stockholm, Sweden..
    Asthma Sub-Phenotyping in Plasma from U-BIOPRED and BIOAIR Using Array-Based Proteomics2019In: American Journal of Respiratory and Critical Care Medicine, ISSN 1073-449X, E-ISSN 1535-4970, Vol. 199Article in journal (Other academic)
  • 4.
    Wiklundh, E.
    et al.
    Karolinska Inst, Dept Med, Stockholm, Sweden..
    Hellström, Cecilia
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Van Moorsel, C. H.
    St Antonius Hosp Pulmonol, Nieuwegein, Netherlands..
    Grutters, J. C.
    St Antonius Hosp, Dept Pulmonol, NL-3435 CM Nieuwegein, Netherlands..
    Nilsson, Peter
    KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science.
    Crestani, B.
    Hop Bichat Claude Bernard, Serv Pneumol, Paris, France..
    The Autoimmune Repertoire in Idiopathic Pulmonary Fibrosis2018In: American Journal of Respiratory and Critical Care Medicine, ISSN 1073-449X, E-ISSN 1535-4970, Vol. 197Article in journal (Other academic)
1 - 4 of 4
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