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  • 1.
    Braun, Stefan
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Wafer-Scale Manufacturing of Bulk Shape-Memory-Alloy Microactuators Based on Adhesive Bonding of Titanium-Nickel Sheets to Structured Silicon Wafers2009Inngår i: Journal of microelectromechanical systems, ISSN 1057-7157, E-ISSN 1941-0158, Vol. 18, nr 6, s. 1309-1317Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This paper presents a concept for the wafer-scale manufacturing of microactuators based on the adhesive bonding of bulk shape-memory-alloy (SMA) sheets to silicon microstructures. Wafer-scale integration of a cold-state deformation mechanism is provided by the deposition of stressed films onto the SMA sheet. A concept for heating of the SMA by Joule heating through a resistive heater layer is presented. Critical fabrication issues were investigated, including the cold-state deformation, the bonding scheme and related stresses, and the titanium-nickel (TiNi) sheet patterning. Novel methods for the transfer stamping of adhesive and for the handling of the thin TiNi sheets were developed, based on the use of standard dicing blue tape. First demonstrator TiNi cantilevers, wafer-level adhesively bonded on a microstructured silicon substrate, were successfully fabricated and evaluated. Intrinsically stressed silicon dioxide and silicon nitride were deposited using plasma-enhanced chemical vapor deposition to deform the cantilevers in the cold state. Tip deflections for 2.5-mm-long cantilevers in cold/hot state of 250/70 and 125/28 mu m were obtained using silicon dioxide and silicon nitride, respectively. The bond strength proved to be stronger than the force created by the 2.5-mm-long TiNi cantilever and showed no degradation after more than 700 temperature cycles. The shape-memory behavior of the TiNi is maintained during the integration process.

  • 2.
    da Silva Granja, Carlos
    et al.
    Centre for Biological Engineering, Loughborough University, UK.
    Sandström, Niklas
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Mikro- och nanosystemteknik.
    Efimov, Igor
    Centre for Biological Engineering, Loughborough University, UK.
    Ostanin, Victor
    Department of Chemistry, University of Cambridge, UK.
    van der Wijngaart, Wouter
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Mikro- och nanosystemteknik.
    Klenerman, David
    Department of Chemistry, University of Cambridge, UK.
    Ghosh, Sourav
    Centre for Biological Engineering, Loughborough University, UK.
    Characterisation of particle-surface interactions via anharmonic acoustic transduction2018Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 272, s. 175-184Artikkel i tidsskrift (Fagfellevurdert)
  • 3.
    de Sousa, Nuno Rufino
    et al.
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden..
    Sandström, Niklas
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik.
    Shen, Lei
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden.;Tongji Univ, Shanghai Pulm Hosp, Sch Med, Shanghai, Peoples R China..
    Hakansson, Kathleen
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden..
    Vezozzo, Rafaella
    Fed Univ Grande Dourados, Fac Hlth Sci, Dourados, MS, Brazil..
    Udekwu, Klas, I
    Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.;SoS BIOS, Uppsala, Sweden..
    Croda, Julio
    Univ Fed Mato Grosso do Sul, Sch Med, Campo Grande, MS, Brazil.;Fundacao Oswaldo Cruz, Campo Grande, MS, Brazil..
    Rothfuchs, Antonio Gigliotti
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden..
    A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols2020Inngår i: Tuberculosis, ISSN 1472-9792, E-ISSN 1873-281X, Vol. 120, artikkel-id 101896Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Tuberculosis (TB) infects about 25% of the world population and claims more human lives than any other infectious disease. TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate.

  • 4.
    Errando-Herranz, Carlos
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik. UPV Polytechnic University of Valencia, Valencia, Spain.
    Saharil, Farizah
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Mola Romero, Albert
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shafagh, Reza Z.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Gylfason, Kristinn B.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Integration of polymer microfluidics with silicon photonic biosensors by one-step combined photopatterning and molding of OSTE2013Konferansepaper (Fagfellevurdert)
    Abstract [en]

    We demonstrate a method for the fast and simple packaging of silicon sensors into a microfluidic package consisting of the recently introduced {OSTE} polymer. The microfluidic layer is first microstructured and thereafter dry-bonded to a silicon photonic sensor, in a process compatible with wafer-level production, and with the entire packaging process lasting only 10 minutes. The fluidic layer combines molded microchannels and fluidic (Luer) connectors with photopatterned through-holes (vias) for optical fiber probing and fluid connections. All the features are fabricated in a single photocuring step. We report measurements with an integrated silicon photonic {Mach-Zehnder} interferometer refractive index sensor packaged by these means.

  • 5.
    Errando-Herranz, Carlos
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Saharil, Farizah
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Mola Romero, Albert
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shafagh, Reza Z.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Wijngaart, Wouter van der
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Gylfason, Kristinn B.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Integration Of Polymer Microfluidic Channels, Vias, And Connectors With Silicon Photonic Sensors By One-Step Combined Photopatterning And Molding Of OSTE2013Inngår i: Proceedings of the 2013 17th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers), IEEE conference proceedings, 2013, s. 1613-1616Konferansepaper (Fagfellevurdert)
    Abstract [en]

    We demonstrate a method for the fast and simple packaging of silicon sensors into a microfluidic package consisting of the recently introduced {OSTE} polymer. The microfluidic layer is first microstructured and thereafter dry-bonded to a silicon photonic sensor, in a process compatible with wafer-level production, and with the entire packaging process lasting only 10 minutes. The fluidic layer combines molded microchannels and fluidic (Luer) connectors with photopatterned through-holes (vias) for optical fiber probing and fluid connections. All the features are fabricated in a single photocuring step. We report measurements with an integrated silicon photonic {Mach-Zehnder} interferometer refractive index sensor packaged by these means.

  • 6.
    Errando-Herranz, Carlos
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Saharil, Farizah
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Romero, Albert Mola
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shafagh, Reza Z.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Wijngaart, Wouter van der
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Gylfason, Kristinn B.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Integration of microfluidics with grating coupled silicon photonic sensors by one-step combined photopatterning and molding of OSTE2013Inngår i: Optics Express, ISSN 1094-4087, E-ISSN 1094-4087, Vol. 21, nr 18, s. 21293-21298Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present a novel integration method for packaging silicon photonic sensors with polymer microfluidics, designed to be suitable for wafer-level production methods. The method addresses the previously unmet manufacturing challenges of matching the microfluidic footprint area to that of the photonics, and of robust bonding of microfluidic layers to biofunctionalized surfaces. We demonstrate the fabrication, in a single step, of a microfluidic layer in the recently introduced OSTE polymer, and the subsequent unassisted dry bonding of the microfluidic layer to a grating coupled silicon photonic ring resonator sensor chip. The microfluidic layer features photopatterned through holes (vias) for optical fiber probing and fluid connections, as well as molded microchannels and tube connectors, and is manufactured and subsequently bonded to a silicon sensor chip in less than 10 minutes. Combining this new microfluidic packaging method with photonic waveguide surface gratings for light coupling allows matching the size scale of microfluidics to that of current silicon photonic biosensors. To demonstrate the new method, we performed successful refractive index measurements of liquid ethanol and methanol samples, using the fabricated device. The minimum required sample volume for refractive index measurement is below one nanoliter.

  • 7.
    Frisk, Thomas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Eng, Lars
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Månsson, Per
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    A miniaturised QCM-based integrated electronic nose system2007Konferansepaper (Annet vitenskapelig)
  • 8.
    Frisk, Thomas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Eng, Lars
    Biosensor Applications AB, Solna.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Månsson, Per
    Biosensor Applications AB, Solna.
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    An integrated QCM-based narcotics sensing microsystem2008Inngår i: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 8, nr 10, s. 1648-1657Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present the design, fabrication and successful testing of a 14 x 14 x 4 mm(3) integrated electronic narcotics sensing system which consists of only four parts. The microsystem absorbs airborne narcotics molecules and performs a liquid assay using an integrated quartz crystal microbalance (QCM). A vertically conductive double-sided adhesive foil (VCAF) was used and studied as a novel material for LOC and MEMS applications and provides easy assembly, electrical contacting and liquid containment. The system was tested for measuring cocaine and ecstasy, with successful detection of amounts as small as 100 ng and 200 ng, respectively These levels are of interest in security activities in customs, prisons and by the police.

  • 9.
    Guha, Arnab
    et al.
    Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Epinal Way, Loughborough, Loughborough University, LE11 3TU, UK.
    Sandström, Niklas
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik.
    Ostanin, Victor
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW , UK.
    van der Wijngaart, Wouter
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Mikro- och nanosystemteknik.
    Klenerman, David
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW , UK.
    Ghosh, Sourav
    Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Epinal Way, Loughborough, Loughborough University, LE11 3TU, UK.
    Measurement of protein binding with vastly improved time resolution using a quartz crystal microbalance driven at a fixed frequency2017Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    Introduction: Quartz crystal microbalance (QCM) is commonly used to study biomolecular binding by measuring shifts in resonance frequency of a quartz-crystal-oscillator. However, the currently used methods like impedance analysis or QCM-D, which require repeated sweeps or ringing, are limited in time resolution (~1 second) due to the need for averaging. This restricts our ability to study transient biomolecular processes, which occur in sub-millisecond time scale. A novel technique has been reported here that allows quantification of resonance frequency of a quartz-crystal-oscillator with significantly improved time resolution by driving and measuring continuously at a constant frequency within the resonance bandwidth. 

    Method: The reactive component of the experimentally obtained impedance is utilized for the estimation of resonance frequency from the Butterworth Van-dyke (BVD) model of a quartz-crystal-oscillator, assuming that changes in motional inductance and capacitance around resonance are negligible. Triplicate sets of experiments involving the binding of streptavidin with a biotin functionalized 14.3 MHz quartz oscillator surface were performed. Intermittent frequency sweeps and fixed frequency drives, both of 0.1 second duration and around 14.3 MHz, were taken at intervals of 2 minutes under the flow of phosphate-buffer-saline (PBS buffer) before and after injection of streptavidin. 

    Results: The average shift in resonance frequency from the baseline (measurements before streptavidin injection) due to streptavidin-biotin binding, calculated from the fixed frequency drive or FFD (148 Hz) was within 1% of that estimated from the frequency sweep method by fitting the experimentally recorded impedance employing the BVD model (149 Hz). 

    Discussion: The agreement of the FFD with conventional frequency sweep method suggests that protein binding can be quantified with reasonable accuracy from each impedance data point, which with our set-up is recorded at 30 kHz sampling rate. This gives a time resolution of 0.03 millisecond, which is about 4 orders of magnitude improvement over the state-of-the-art.

  • 10.
    Guha, Arnab
    et al.
    Centre for Biological Engineering, Loughborough University, Loughborough, UK.
    Sandström, Niklas
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Mikro- och nanosystemteknik.
    Ostanin, Victor
    Department of Chemistry, University of Cambridge, Cambridge, UK.
    van der Wijngaart, Wouter
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Mikro- och nanosystemteknik.
    Klenerman, David
    Department of Chemistry, University of Cambridge, Cambridge, UK.
    Ghosh, Sourav
    Centre for Biological Engineering, Loughborough University, Loughborough, UK.
    Simple and ultrafast resonance frequency and dissipation shift measurements using a fixed frequency drive2018Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 281, s. 960-970Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A new method for determination of resonance frequency and dissipation of a mechanical oscillator is presented. Analytical expressions derived using the Butterworth-Van Dyke equivalent electrical circuit allow the determination of resonance frequency and dissipation directly from each impedance datapoint acquired at a fixed amplitude and frequency of drive, with no need for numerical fitting or measurement dead time unlike the conventional impedance or ring-down analysis methods. This enables an ultrahigh time resolution and superior noise performance with relatively simple instrumentation. Quantitative validations were carried out successfully against the impedance analysis method for inertial and viscous loading experiments on a 14.3 MHz quartz crystal resonator (QCR). Resonance frequency shifts associated with the transient processes of quick needle touches on a thiol self-assembled-monolayer functionalised QCR in liquid were measured with a time resolution of 112 μs, which is nearly two orders of magnitude better than the fastest reported quartz crystal microbalance. This simple and fast fixed frequency drive (FFD) based method for determination of resonance frequency and dissipation is potentially more easily multiplexable and implementable on a single silicon chip delivering economies of scale.

  • 11.
    Gylfason, Kristinn B.
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Errando-Herranz, Carlos
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shafagh, Reza Zandi
    Wijngaart, Wouter van der
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Integration of polymer based microfluidics with silicon photonics for biosensing applications2015Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    We present a novel integration method for packaging silicon photonic sensors with polymer microfluidics, designed to be suitable for wafer-level production. The method addresses the previously unmet manufacturing challenges of matching the microfluidic footprint area to that of the photonics, and of robust bonding of microfluidic layers to biofunctionalized surfaces. We demonstrate the fabrication, in a single step, of a microfluidic layer in the recently introduced OSTE polymer, and the subsequent unassisted dry bonding of the microfluidic layer to a grating coupled silicon photonic ring resonator sensor chip. The microfluidic layer features photopatterned through holes (vias) for optical fiber probing and fluid connections, as well as molded microchannels and tube connectors, and is manufactured and subsequently bonded to a silicon sensor chip in less than 10 minutes. Combining this new microfluidic packaging method with photonic waveguide surface gratings for light couplin g allows matching the size scale of microfluidics to that of current silicon photonic biosensors.

  • 12.
    Hill, Daniel
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Gylfason, Kristinn
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Carlborg, Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Karlsson, J. Mikael
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sohlström, Hans B.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Russom, Aman
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Claes, T.
    Bienstman, P.
    Kazmierczak, A.
    Dortu, F.
    Banuls Polo, M. J.
    Maquieira, A.
    Kresbach, G. M.
    Vivien, L.
    Popplewell, J.
    Ronan, G.
    Barrios, C. A.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Microfluidic and Transducer Technologies for Lab on a Chip Applications2010Inngår i: 2010 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC), IEEE conference proceedings, 2010, s. 305-307Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Point-of-care diagnostic devices typically require six distinct qualities: they must deliver at least the same sensitivity and selectivity, and for a cost per assay no greater than that of today's central lab technologies, deliver results in a short period of time (<15 min at GP; <2h in hospital), be portable or at least small in scale, and require no or extremely little sample preparation. State-of-the-art devices deliver information of several markers in the same measurement.

  • 13.
    Karlsson, J. Mikael
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Russom, Aman
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
    Wijngaart, Wouter van der
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    On-Chip Liquid Degassing With Low Water Loss2010Inngår i: Proceedings Micro Total Analysis Systems (μTAS) 2010, Groningen: CBMS , 2010, s. 1790-1792Konferansepaper (Fagfellevurdert)
    Abstract [en]

    We present a novel approach for actively degassing liquid and removing trapped air bubbles in microfluidic devices.

    In our approach, an integrated gas permeable membrane, consisting of a structurally supporting PDMS layer that is covered with a thin Teflon® AF 1600 film, separates the on-chip liquid from an on-chip low-vacuum chamber. Since the Teflon AF permeability is near zero for liquid water and low for vapour, air bubbles and dissolved air are removed through the membrane whilst the loss of water, ions and biomolecules in the system remains low. The system has been demonstrated at elevated temperatures and could be suitable for e.g. degassing during on-chip PCR.

  • 14.
    Leirs, Karen
    et al.
    KU Leuven.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Ladhani, Laila
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Spasic, Dragana
    KU Leuven.
    Ostanin, Victor
    University of Cambridge.
    Klenerman, David
    University of Cambridge.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Ghosh, Sourav
    Loughborough University.
    Lammertyn, Jeroen
    KU Leuven.
    Screening of antibodies for the development of a fast and sensitivie influenza: A nucleoprotein detection on a nonlinear acoustic sensor2014Konferansepaper (Fagfellevurdert)
  • 15.
    Leirs, Karen
    et al.
    MeBioS - Biosensor group, KU Leuven.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Ladhani, Laila
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Spasic, Dragana
    MeBioS - Biosensor group, KU Leuven.
    Ostanin, Victor
    University of Cambridge.
    Klenerman, David
    University of Cambridge.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Lammertyn, Jeroen
    KU Leuven.
    Ghosh, Sourav
    Loughborough University.
    Rapid ultra-sensitive detection of influenza A nucleoproteins using a microfluidic nonlinear acoustic sensor2014Konferansepaper (Fagfellevurdert)
  • 16.
    Pardon, Gaspard
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Ladhani, Laila
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Ettori, Maxime
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Lobov, Gleb
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Aerosol sampling using an electrostatic precipitator integrated with a microfluidic interface2015Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 212, s. 344-352Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this work, the development of a point-of-care (PoC) system to capture aerosol from litres of air directly onto a microfluidic lab-on-chip for subsequent analysis is addressed. The system involves an electrostatic precipitator that uses corona charging and electrophoretic transport to capture aerosol droplets onto a microfluidic air-to-liquid interface for downstream analysis. A theoretical study of the governing geometric and operational parameters for optimal electrostatic precipitation is presented. The fabrication of an electrostatic precipitator prototype and its experimental validation using a laboratory-generated aerosolized dye is described. Collection efficiencies were comparable to those of a state-of-the-art Biosampler impinger, with the significant advantage of providing samples that are at least 10 times more concentrated. Finally, we discuss the potential of such a system for breath-based diagnostics.

  • 17.
    Prager, Isabel
    et al.
    Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, Dortmund, Germany.
    Liesche, Clarissa
    Division of Theoretical Bioinformatics, German Cancer Research Center and BioQuant Center, Heidelberg, Germany.
    van Ooijen, Hanna
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Urlaub, Doris
    Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, Dortmund, Germany.
    Verron, Quentin
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Sandström, Niklas
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Fasbender, Frank
    Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, Dortmund, Germany.
    Claus, Maren
    Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, Dortmund, Germany.
    Eils, Roland
    Division of Theoretical Bioinformatics, German Cancer Research Center and BioQuant Center, Heidelberg, Germany.
    Beaudouin, Joël
    Division of Theoretical Bioinformatics, German Cancer Research Center and BioQuant Center, Heidelberg, Germany.
    Önfelt, Björn
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Watzl, Carsten
    Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, Dortmund, Germany.
    NK cells switch from granzyme B to death receptor–mediated cytotoxicity during serial killing2019Inngår i: Journal of Experimental Medicine, ISSN 0022-1007, E-ISSN 1540-9538, Vol. 7, nr 9, s. 2113-2127Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    NK cells eliminate virus-infected and tumor cells by releasing cytotoxic granules containing granzyme B (GrzB) or by engaging death receptors that initiate caspase cascades. The orchestrated interplay between both cell death pathways remains poorly defined. Here we simultaneously measure the activities of GrzB and caspase-8 in tumor cells upon contact with human NK cells. We observed that NK cells switch from inducing a fast GrzB-mediated cell death in their first killing events to a slow death receptor–mediated killing during subsequent tumor cell encounters. Target cell contact reduced intracellular GrzB and perforin and increased surface-CD95L in NK cells over time, showing how the switch in cytotoxicity pathways is controlled. Without perforin, NK cells were unable to perform GrzB-mediated serial killing and only killed once via death receptors. In contrast, the absence of CD95 on tumor targets did not impair GrzB-mediated serial killing. This demonstrates that GrzB and death receptor–mediated cytotoxicity are differentially regulated during NK cell serial killing.

  • 18.
    Samel, Björn
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Griss, Patrick
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    A laterally operating liquid aspiration and dispensing unit based on an expanding PDMS composite2007Inngår i: Micro Total Analysis Systems (muTAS), 2007, Vol. 1, s. 530-532Konferansepaper (Fagfellevurdert)
  • 19.
    Samel, Björn
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Griss, Patrick
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Liquid Aspiration and Dispensing Based on an Expanding PDMS Composite2008Inngår i: Journal of microelectromechanical systems, ISSN 1057-7157, E-ISSN 1941-0158, Vol. 17, nr 5, s. 1254-1262Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this paper, we present the development of active liquid aspiration and dispensing units designed for vertical, as well as lateral, liquid aspiration. The devices are based on a single-use thermally expanding polydimethylsiloxane (PDMS) composite, which allows altering its surface topography by means of individually addressable integrated heaters. Devices are designed in order to create an enclosed cavity in the system, due to locally expanding the initially unstructured composite. This enables negative volume displacement and leads to the event of liquid aspiration. To enable this device functionality, two different techniques of selectively creating permanent PDMS bonds have been developed. One approach utilizes the plasma-assisted PDMS bonding technique, together with a patterned antistiction layer to form reversibly, as well as irreversibly, bonded regions. Another approach utilizes microcontact printing of PDMS curing agent, which serves as a patterned intermediate layer for adhesive bonding. Fabricated prototype devices successfully demonstrated the aspiration and release of liquid volumes ranging from 28 to 815 nL. The devices are entirely fabricated from low-cost materials, using wafer-level processes only and do not require external means for liquid actuation.

  • 20.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Integrating Biosensors for Air Monitoring and Breath-Based Diagnostics2015Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The air we breathe is the concern of all of us but nevertheless we only know very little about airborne particles, and especially which biological microorganisms they contain. Today, we live in densely populated societies with a growing number of people, making us particularly vulnerable to air transmission of pathogens. With the recent appearance of highly pathogenic types of avian influenza in southeast Asia and the seasonal outbreaks of gastroenteritis caused by the extremely contagious norovirus, the need for portable, sensitive and rapid instruments for on-site detection and monitoring of airborne pathogens is apparent.

    Unfortunately, the integration incompatibility between state-of-the-art air sampling techniques and laboratory based analysis methods makes instruments for in-the-field rapid detection of airborne particles an unresolved challenge.

    This thesis aims at addressing this challenge by the development of novel manufacturing, integration and sampling techniques to enable the use of label-free biosensors for rapid and sensitive analysis of airborne particles at the point-of-care or in the field.

    The first part of the thesis introduces a novel reaction injection molding technique for the fabrication of high quality microfluidic cartridges. In addition, electrically controlled liquid aspiration and dispensing is presented, based on the use of a thermally actuated polymer composite integrated with microfluidic cartridges.

    The second part of the thesis demonstrates three different approaches of biosensor integration with microfluidic cartridges, with a focus on simplifying the design and integration to enable disposable use of the cartridges.

    The third part to the thesis presents a novel air sampling technique based on electrophoretic transport of airborne particles directly to microfluidic cartridges. This technique is enabled by the development of a novel microstructured component for integrated air-liquid interfacing. In addition, a method for liquid sample mixing with magnetic microbeads prior to downstream biosensing is demonstrated.In the fourth part of the thesis, three different applications for airborne particle biosensing are introduced and preliminary experimental results are presented.

  • 21.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Braun, Stefan
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Grund, T.
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Kohl, M.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Wafer-Scale Manufacturing of Robust Trimorph Bulk SMA Microactuators2008Inngår i: ACTUATOR 08, CONFERENCE PROCEEDINGS / [ed] Borgmann H., 2008, s. 382-385Konferansepaper (Fagfellevurdert)
    Abstract [en]

    This paper demonstrates the concept of wafer-level fabrication and integration of robust bulk SMA microactuators based on selective adhesive bonding of cold-rolled SMA sheets to silicon wafers. Contact printing of an adhesive polymer ensures a selective bonding when transferring full SMA sheets to silicon structures on a patterned wafer. The induced stress of a thin dielectric film deposited on top of the SMA sheet ensures a stable and built-in reset mechanism of the actuators. The trimorph microactuators can be actuated by indirect resistive heating using electrical current through a thin metal film. We report on the successful wafer-scale fabrication of actuator cantilevers and their characteristics. First test cantilevers show a cold-state deflection of 300 mu m which, however, is limited by the silicon substrate. Upon heating, the cantilever shows a stroke of approx. 80 mu m.

  • 22.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Braun, Stefan
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    FULL WAFER INTEGRATION OF SHAPE MEMORY ALLOY MICROACTUATORS USING ADHESIVE BONDING2009Inngår i: 15th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems (IEEE TRANSDUCERS 2009), IEEE conference proceedings, 2009, s. 845-848Konferansepaper (Fagfellevurdert)
    Abstract [en]

    This paper presents the wafer-scale manufacturing of microactuators based on bulk shape memory alloy material integrated using adhesive bonding. The work addresses key technical challenges related to the wafer-scale fabrication of bulk SMA micro actuators, including wafer-scale integration of patterned SMA sheets to structured Si wafers and the integration of cold state reset layers to the microactuators. Contact printing of an adhesive polymer ensures a selective bonding when transferring full SMA sheets to silicon structures on a patterned wafer. The stressed films deposited on top of the SMA microactuator ensure a built-in reset mechanism of the actuators. The paper reports on the successful wafer-scale integration of wafer-sized SMA sheets and the wafer-scale fabrication of actuator cantilevers. First test cantilevers with a length of 2.5 mm show a stroke of approx. 180 ï¿œm.

  • 23.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Frisk, Thomas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Electrohydrodynamic Enhanced Transport and Trapping of Airborne Particles to a Microfluidic Air-Liquid Interface2008Inngår i: Micro System Workshop 2008, 2008Konferansepaper (Annet vitenskapelig)
  • 24.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Frisk, Thomas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    ELECTROHYDRODYNAMIC ENHANCED TRANSPORT AND TRAPPING OF AIRBORNE PARTICLES TO A MICROFLUIDIC AIR-LIQUID INTERFACE2008Inngår i: 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), IEEE conference proceedings, 2008, s. 595-598Konferansepaper (Fagfellevurdert)
    Abstract [en]

    We introduce a novel approach for greatly improved transport and trapping of airborne sample to a microfluidic analysis system by integrating an electrohydrodynamic (EHD) air pump with a microfluidic air-liquid interface. In our system, a negative corona discharge partially ionizes the air around a sharp electrode tip while the electrostatic field accelerates airborne particles towards an electrically grounded liquid surface, where they absorb. The air-liquid interface is fixated at the microscale pores of a perforated silicon diaphragm, each pore functioning as a static Laplace valve. Our system was experimentally tested using airborne smoke particles of ammonium chloride and aqueous salt solution as the liquid. We measured that EHD enhanced transport of the particles from the air into the liquid is enhanced over 130 times compared to passive trapping.

  • 25.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Frisk, Thomas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Microsystems for Airborne Sample Detection2008Inngår i: Mini-symposium on Opto-Microfluidics for Biological Large Scale Integration, 2008Konferansepaper (Annet vitenskapelig)
  • 26.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Gylfason, Kristinn B.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Carlborg, Carl Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Karlsson, Mikael
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sohlström, Hans
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Russom, Aman
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
    Hill, Daniel
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Wijngaart, Wouter van der
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Microfluidic systems for point-of-care diagnostics2010Inngår i: 8th Micronano System Workshop, MSW 2010, 2010Konferansepaper (Annet vitenskapelig)
  • 27.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Gylfason, Kristinn
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Carlborg, Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Karlsson, J. Mikael
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Sohlström, Hans B.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Russom, Aman
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
    Hill, Daniel
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Lab-on-a-chip microsystems for point-of-care diagnostics2010Inngår i: 1st International scientific conference on Microfluidics in bioanalytical research and diagnostics, 2010, s. 34-35Konferansepaper (Annet vitenskapelig)
  • 28.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shafagh, Reza
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Carlborg, Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Integration of a QCM with an OSTE cartridge2012Inngår i: MicroNano System Workshop 2012: 9th Micronano System Technology Event, Linköping, 2012Konferansepaper (Annet vitenskapelig)
  • 29.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shafagh, Reza
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Carlborg, Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Simple integration of a biosensor with an OSTE polymer cartridge by low temperature dry bonding2012Inngår i: Medicinteknikdagarna 2012, Lund, 2012Konferansepaper (Annet vitenskapelig)
  • 30.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Shafagh, Reza Zandi
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Carlborg, Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik (Bytt namn 20121201).
    ONE STEP INTEGRATION OF GOLD COATED SENSORS WITH OSTE POLYMER CARTRIDGES BY LOW TEMPERATURE DRY BONDING2011Inngår i: 16th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems (IEEE TRANSDUCERS 2011), IEEE conference proceedings, 2011, s. 2778-2781Konferansepaper (Fagfellevurdert)
    Abstract [en]

    We propose and demonstrate a novel one step method to integrate gold coated sensors with cartridges by dry covalent bonding. The cartridges are replica molded in an UV-curable off-stoichiometry thiol-ene (OSTE) polymer, featuring an excess of thiol functional groups that covalently bond to the surface of gold coated sensors upon contact. The method is demonstrated by the integration of a gold coated quartz crystal to a microfludic OSTE cartridge. The resulting bond interface is shown to be completely homogenous and void free and the package is tested successfully to a differential pressure of up to 2 bars. The performance of the biosensor chip is evaluated by measuring the unspecific binding of 0.5% albumin, resulting in a total frequency drop 205 Hz. This approach delivers a simple but rapid high quality integration aiming for the production of robust, low cost and disposable biosensor chips

  • 31.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Zandi Shafagh, Reza
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Gylfason, Kristinn
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Batch fabrication of polymer microfluidic cartridges for QCM sensor packaging by direct bonding2017Inngår i: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 27, nr 12, artikkel-id 124001Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Quartz crystal microbalance (QCM) sensing is an established technique commonly used in laboratory based life-science applications. However, the relatively complex, multi-part design and multi-step fabrication and assembly of state-of-the-art QCM cartridges makes them unsuited for disposable applications such as point-of-care (PoC) diagnostics. In this work, we present the uncomplicated manufacturing of QCMs in polymer microfluidic cartridges. Our novel approach comprises two key innovations: the batch reaction injection molding of microfluidic parts; and the integration of the cartridge components by direct, unassisted bonding. We demonstrate molding of batches of 12 off-stoichiometry thiol-ene epoxy polymer (OSTE+) polymer parts in a single molding cycle using an adapted reaction injection molding process; and the direct bonding of the OSTE+ parts to other OSTE+ substrates, to printed circuit boards, and to QCMs. The microfluidic QCM OSTE+ cartridges were successfully evaluated in terms of liquid sealing as well as electrical properties, and the sensor performance characteristics are on par with those of commercially available QCM biosensor cartridge.

  • 32.
    Sandström, Niklas
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Zandi Shafagh, Reza
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Vastesson, Alexander
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Carlborg, Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Reaction injection molding and direct covalent bonding of OSTE+ polymer microfluidic devices2015Inngår i: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 25, nr 7Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this article, we present OSTE+RIM, a novel reaction injection molding (RIM) process that combines the merits of off-stoichiometric thiol–ene epoxy (OSTE+) thermosetting polymers with the fabrication of high quality microstructured parts. The process relies on the dual polymerization reactions of OSTE+ polymers, where the first curing step is used in OSTE+RIM for molding intermediately polymerized parts with well-defined shapes and reactive surface chemistries. In the facile back-end processing, the replicated parts are directly and covalently bonded and become fully polymerized using the second curing step, generating complete microfluidic devices. To achieve unprecedented rapid processing, high replication fidelity and low residual stress, OSTE+RIM uniquely incorporates temperature stabilization and shrinkage compensation of the OSTE+ polymerization during molding. Two different OSTE+ formulations were characterized and used for the OSTE+RIM fabrication of optically transparent, warp-free and natively hydrophilic microscopy glass slide format microfluidic demonstrator devices, featuring a storage modulus of 2.3 GPa and tolerating pressures of at least 4 bars. 

  • 33.
    Vastesson, Alexander
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Zhou, Xiamo
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Saharil, Farizah
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Supekar, Omkar
    Indian Institute of Technology, Bombay, India.
    Stemme, Göran
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    ROBUST MICRODEVICE MANUFACTURING BY DIRECT LITHOGRAPHY AND ADHESIVE-FREE BONDING OF OFF-STOICHIOMETRY THIOL-ENE-EPOXY (OSTE+) POLYMER2013Inngår i: 17th IEEE International Conference on Solid-State Sensors, Actuators and Microsystems & EUROSENSORS XXVII (IEEE TRANSDUCERS 2013), IEEE conference proceedings, 2013, s. 408-411Konferansepaper (Fagfellevurdert)
    Abstract [en]

    We here demonstrate, for the first time, the use of direct lithography in off-stoichiometry thiol-ene-epoxy (OSTE+) to fabricate a microdevice. First, the photolithographic property of OSTE+ is shown by using a photomask to create micropillars with an aspect-ratio of 1:10 in a 2 mm thick layer. Secondly, a three-layer OSTE+ microdevice containing in-/outlet holes, channels, and pillars is fabricated by using a combination of direct lithography and adhesive-free dry bonding. The resulting microdevice shows desirable properties, such as leak-free filling and hydrophilic surfaces. This fabrication method enhances the microstructurability of OSTE+ beyond that of conventional soft lithography replica molding of other polymers, such as PDMS.

  • 34.
    Wijngaart, Wouter van der
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Despont, R. Michel
    Bhattacharyya, Debrabata
    Kirby, Paul B.
    Wilson, Stephen
    Jourdain, Roland
    Braun, Stefan
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Barth, Johannes
    Grund, Thomas
    Kohl, Manfred
    Niklaus, Frank
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Lapisa, Martin
    KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
    Zimmer, Fabian
    Increasing the Performance per Cost of Microsystems by Transfer Bonding Manufacturing Techniques2008Annet (Annet vitenskapelig)
  • 35.
    Zhou, Xiamo
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Carlborg, Carl Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haleem, A.
    KTH.
    Vastesson, Alexander
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Saharil, Farizah
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Rapid Fabrication Of OSTE+ Microfluidic Devices With Lithographically Defined Hydrophobic/ Hydrophilic Patterns And Biocompatible Chip Sealing2013Inngår i: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany: NO , 2013, s. 134-136Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Here we present an uncomplicated and robust method for rapid prototyping of microfluidic devices featuring: 1) lithographically defined, permanently surface modified hydrophilic and hydrophobic regions with contact angles varying from 18o to 118o, in which all four channel walls are surface modified in a single step using polymer chain grafting; 2) polymer chains grafted from open surfaces before bonding, making this method suitable for batch fabrication; 3) biomolecule-compatible, room temperature, dry, homogeneous chip-sealing, in which native as well as hydrophobic/hydrophilic modified OSTE+ surfaces allow for epoxy-epoxy and thiol-epoxy covalent bonding, hence greatly simplifying alignment and dramatically increasing device yields. We demonstrate the method with a functional microfluidic device. This represents a complete, simplified and robust method for batch-manufacturing compatible prototyping of microfluidic devices with tunable mechanical and surface properties. 

  • 36.
    Zhou, Xiamo
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Carlborg, Carl Fredrik
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Sandström, Niklas
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Vastesson, Alexander
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Saharil, Farizah
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    van der Wijngaart, Wouter
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Haraldsson, Tommy
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    OSTE+ microfluidic devices with lithographically defined hydrophobic/ hydrophilic patterns and biocompatible chip sealing: OSTEmer Allows Easy Fabrication of Microfluidic Chips2014Inngår i: DIATECH 2014, 2014, s. 26-27Konferansepaper (Fagfellevurdert)
1 - 36 of 36
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