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  • 1. Hussain, M.
    et al.
    Zhou, Yang
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology. Albanova University Center.
    Song, Y.
    Hameed, H. M. A.
    Jiang, H.
    Tu, Yaoquan
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology. Albanova University Center.
    Zhang, J.
    ATAD2 in cancer: a pharmacologically challenging but tractable target2018In: Expert opinion on therapeutic targets, ISSN 1472-8222, E-ISSN 1744-7631, Vol. 22, no 1, p. 85-96Article in journal (Refereed)
    Abstract [en]

    Introduction: ATAD2 protein is an emerging oncogene that has strongly been linked to the etiology of multiple advanced human cancers. Therapeutically, despite the fact that genetic suppression/knockdown studies have validated it as a compelling drug target for future therapeutic development, recent druggability assessment data suggest that direct targeting of ATAD2’s bromodomain (BRD) may be a very challenging task. ATAD2’s BRD has been predicted as a ‘difficult to drug’ or ‘least druggable’ target due to the concern that its binding pocket, and the areas around it, seem to be unfeasible for ligand binding. Areas covered: In this review, after shedding light on the multifaceted roles of ATAD2 in normal physiology as well as in cancer-etiology, we discuss technical challenges rendered by ATAD2’s BRD active site and the recent drug discovery efforts to find small molecule inhibitors against it. Expert opinion: The identification of a novel low-nanomolar semi-permeable chemical probe against ATAD2’s BRD by recent drug discovery campaign has demonstrated it to be a pharmacologically tractable target. Nevertheless, the development of high quality bioavailable inhibitors against ATAD2 is still a pending task. Moreover, ATAD2 may also potentially be utilized as a promising target for future development of RNAi-based therapy to treat cancers. 

  • 2.
    Kuang, Guanglin
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Murugan, N. Arul
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Nordberg, Agneta
    Karolinska Inst, Ctr Alzheimer Res, Dept Neurobiol Care Sci & Soc, Div Clin Geriatr, S-17177 Stockholm, Sweden.;Karolinska Univ Hosp, Aging Theme, S-14186 Stockholm, Sweden..
    Ågren, Hans
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology. Henan Univ, Coll Chem & Chem Engn, Kaifeng 475004, Henan, Peoples R China..
    Computational Insight into the Binding Profile of the Second-Generation PET Tracer PI2620 with Tau Fibrils2020In: ACS Chemical Neuroscience, E-ISSN 1948-7193, Vol. 11, no 6, p. 900-908Article in journal (Refereed)
    Abstract [en]

    Abnormal deposition of hyperphosphorylated tau as neurofibrillary tangles (NFTs) is an important pathological hallmark of Alzheimer's disease (AD) and of other neurodegenerative disorders. A noninvasive positron emission tomography (PET) tracer that quantifies neurofibrillary tangles in vivo can enhance the clinical diagnosis of AD and can also be used to evaluate the efficacy of therapeutics aimed at reducing the abnormal aggregation of the tau fibril in the brain. In this paper, we study the binding profile of fibrillar tau aggregates with a PET tracer PI2620, which is a new second generation tau PET tracer that is presently experimentally and clinically studied. The target structure for the tau fibril is based on cryo-electron microscopy (cryo-EM) structure. A multiscale simulation workflow including molecular docking, molecular dynamics simulation, metadynamics simulation, and free energy calculations was implemented. We find that PI2620 can bind to eight surface binding sites, three core binding sites, and one entry site. The binding at the core sites and entry site is found to be much more favorable than that on the surface sites due to stronger hydrophobic interactions and less solvent exposure. Furthermore, the entry site which is formed by the terminal beta-sheets of the fibril is found to have the highest binding affinity to PI2620. Importantly, the binding capacity at the entry site can be much higher than that at other core sites, due to its easy accessibility. Therefore, the entry site is believed to be the major binding site for PI2620. A previous computational study on tracers with tau fibrils reports a maximum of four binding sites. Through use of methods that allow us to locate "cryptic binding sites", we report here additional core sites available for binding and we address the limitation of using the cryo-EM structure alone for structure-based tracer design. Our results could be helpful for elucidating the binding mechanism of imaging tracers with the fibrillar form of tau, a knowledge that in turn can be used to guide the development of compounds with higher affinity and selectivity for tau using structure-based design strategies.

  • 3.
    Kuang, Guanglin
    et al.
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
    Zhou, Yang
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
    Zou, Rongfeng
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
    Halldin, C.
    Nordberg, A.
    Långström, B.
    Ågren, Hans
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology. Siberian Federal University, Russian Federation.
    Tu, Yaoquan
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
    Characterization of the binding mode of the PET tracer [18F]ASEM to a chimera structure of the α7 nicotinic acetylcholine receptor2017In: RSC Advances, E-ISSN 2046-2069, Vol. 7, no 32, p. 19787-19793Article in journal (Refereed)
    Abstract [en]

    The α7 nicotinic acetylcholine receptor (α7-nAChR) is assumed to be implicated in a variety of neurological disorders, such as schizophrenia and Alzheimer's disease (AD). The progress of these disorders can be studied through imaging α7-nAChR with positron emission tomography (PET). [18F]ASEM is a novel and potent α7-nAChR PET radioligand showing great promise in recent tests. However, the mechanism of the molecular interaction between [18F]ASEM and α7-nAChR is still unclear. In this paper, the binding profile of [18F]ASEM to a chimera structure of α7-nAChR was investigated with molecular docking, molecular dynamics, and metadynamics simulation methods. We found that [18F]ASEM binds at the same site as the crystallized agonist epibatidine but with a different binding mode. The dibenzo[b,d]thiophene ring has a different orientation compared to the pyridine ring of epibatidine and has van der Waals interactions with residues from loop C on one side and π-π stacking interaction with Trp53 on the other side. The conformation of Trp53 was found to have a great impact on the binding of [18F]ASEM. Six binding modes in terms of the side chain dihedral angles χ1 and χ2 of Trp53 were discovered by metadynamics simulation. In the most stable binding mode, Trp53 adopts a different conformation from that in the crystalline structure and has a rather favorable π-π stacking interaction with [18F]ASEM. We believe that these discoveries can be valuable for the development of novel PET radioligands.

  • 4.
    Li, Junhao
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Tang, Yun
    East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China..
    Li, Weihua
    East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China..
    Tu, Yaoquan
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Dissecting the Structural Plasticity and Dynamics of Cytochrome P450 2B4 by Molecular Dynamics Simulations2020In: Journal of Chemical Information and Modeling, ISSN 1549-9596, E-ISSN 1549-960X, Vol. 60, no 10, p. 5026-5035Article in journal (Refereed)
    Abstract [en]

    The plasticity of cytochromes P450 (P450s) is known to contribute significantly to their catalytic capacity of metabolizing various substrates. Although numerous studies have been performed, factors governing the plasticity and dynamics of P450s are still not fully understood. In this study, taking CYP2B4 as an example, we dissect the protein plasticity and dynamics in different environments. CYP2B4 is featured by a high degree of plasticity, which exhibits open, closed, and intermediate states. By analyzing the CYP2B4 crystal structures, we identified the structural features for the closed, open, and intermediate states. Interestingly, formation of the dimer structure was found in the open and intermediate states. The subsequent molecular dynamics (MD) simulations of the open structure in water confirmed the importance of the dimer form in stabilizing the open conformations. MD simulations of the closed and open structures in the membrane environment and the free energies for opening the F-G cassette obtained from the umbrella sampling calculations indicate that the membrane environment is important for stabilizing the F-G cassette. The dynamical network analysis indicates that Asp105 on the B-C loop plays an important role in transiting the structure from the open to the intermediate state. Our results thus unveil the mechanisms of dimer formation and open-to-intermediate transition for CYP2B4 in the water and membrane environments.

  • 5.
    Li, Junhao
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Tang, Yun
    East China University of Science and Technology.
    Li, Weihua
    East China University of Science and Technology.
    Tu, Yaoquan
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Dissecting the Structural Plasticity and Dynamics of Cytochrome P450 2B4 by Molecular Dynamics SimulationsManuscript (preprint) (Other academic)
    Abstract [en]

    The plasticity of cytochrome P450 enzymes (P450s) is known to contribute significantly to their catalytic capacity of metabolizing various substrates. Although numerous studies have been performed, factors governing the plasticity and dynamics of P450s are still not fully understood. In this study, taking CYP2B4 as an example, we dissect the protein plasticity and dynamics in different environments. CYP2B4 is featured by a high degree of plasticity that exhibits open, closed, and intermediate states. By analyzing the CYP2B4 crystal structures, we identified the structural features for the closed, open and intermediate states. Interestingly, formation of the dimer structure was found in the open and intermediate structures. The subsequent MD simulations of the open structure in water confirmed the importance of the dimer form in stabilizing the open conformations. MD simulations of the closed and open structures in the membrane environment and the free energies for opening the F-G cassette obtained from the umbrella sampling calculations indicate that the membrane environment is important for stabilizing the F-G cassette. The dynamical network analysis indicates that Asp105 on the B-C loop plays an important role in transiting the structure from the open to intermediate. Our results thus unveil the mechanism of dimer formation and open-to-intermediate transition for CYP2B4 in the water and membrane environments.

  • 6.
    Makafe, Gaelle G.
    et al.
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Hussain, Muzammal
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Chinese Acad Sci, Guangdong Prov Key Lab Biocomp, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Surineni, Goverdhan
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Tan, Yaoju
    Guangzhou Chest Hosp, Dept Clin Lab, State Key Lab Resp Dis, 62 Hengzhigang Rd, Guangzhou 510095, Guangdong, Peoples R China..
    Wong, Nai-Kei
    Southern Univ Sci & Technol, Hosp 2, Shenzhen Peoples Hosp 3, Key Discipline Infect Dis, 29 Bulan Rd, Shenzhen 518112, Peoples R China..
    Julius, Mugweru
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Embu, Dept Biol Sci, Embu 660100, Kenya..
    Liu, Lanying
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Gift, Chiwala
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Jiang, Huofeng
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Sci & Technol China, Sch Life Sci, 96 Jinzhai Rd, Hefei 230027, Anhui, Peoples R China..
    Tang, Yunxiang
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Anhui Univ, Inst Phys Sci & Informat Technol, 111 Jiulong Rd, Hefei 230009, Anhui, Peoples R China..
    Liu, Jianxiong
    Guangzhou Chest Hosp, Dept Clin Lab, State Key Lab Resp Dis, 62 Hengzhigang Rd, Guangzhou 510095, Guangdong, Peoples R China..
    Tan, Shouyong
    Guangzhou Chest Hosp, Dept Clin Lab, State Key Lab Resp Dis, 62 Hengzhigang Rd, Guangzhou 510095, Guangdong, Peoples R China..
    Yu, Zhijun
    Chinese Acad Sci, Guangdong Prov Key Lab Biocomp, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Liu, Zhiyong
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China..
    Lu, Zhili
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China..
    Fang, Cuiting
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Zhang, Jiancun
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Chinese Acad Sci, Guangdong Prov Key Lab Biocomp, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Zhu, Qiang
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Liu, Jinsong
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Chinese Acad Sci, Guangdong Prov Key Lab Biocomp, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Zhang, Tianyu
    Chinese Acad Sci, State Key Lab Resp Dis, Guangzhou Inst Biomed & Hlth, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Guangzhou Regenerat Med & Hlth Guangdong Lab GRM, 190 Kaiyuan Ave,Sci Pk, Guangzhou 510530, Guangdong, Peoples R China.;Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China..
    Quinoline Derivatives Kill Mycobacterium tuberculosis by Activating Glutamate Kinase2019In: Cell Chemical Biology, ISSN 2451-9456, E-ISSN 2451-9448, Vol. 26, no 8, p. 1187-+Article in journal (Refereed)
    Abstract [en]

    There is a great need for identification and development of new anti-tuberculosis drugs with novel targets. Recent drug-discovery efforts typically focus on identifying inhibitors but not activators that perturb metabolic enzymes' functions as a means to kill Mycobacterium tuberculosis (Mtb). Here, we describe a class of quinoline compounds, Z0933/Z0930, which kill Mtb by acting as activators of glutamate kinase (GK), a previously untargeted enzyme catalyzing the first step of proline biosynthesis. We further show that Z0933/Z0930 augment proline production and induce Mtb killing via proline-derived redox imbalance and production of reactive oxygen species. This work highlights the effectiveness of gain-of-function probes against Mtb and provides a framework for the discovery of next-generation allosteric activators of GK.

  • 7.
    Ti, Huihui
    et al.
    Guangzhou Med Univ, Key Lab Mol Target & Clin Pharmacol, State Key Lab Resp Dis, Sch Pharmaceut Sci, Guangzhou 511436, Guangdong, Peoples R China.;Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou 511436, Guangdong, Peoples R China..
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology. Guangzhou Med Univ, Key Lab Mol Target & Clin Pharmacol, State Key Lab Resp Dis, Sch Pharmaceut Sci, Guangzhou 511436, Guangdong, Peoples R China.;Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou 511436, Guangdong, Peoples R China.
    Liang, Xue
    Guangzhou Med Univ, Key Lab Mol Target & Clin Pharmacol, State Key Lab Resp Dis, Sch Pharmaceut Sci, Guangzhou 511436, Guangdong, Peoples R China.;Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou 511436, Guangdong, Peoples R China..
    Li, Runfeng
    Guangzhou Med Univ, State Key Lab Resp Dis, Natl Clin Res Ctr Resp Dis, Guangzhou Inst Resp Hlth,Affiliated Hosp 1, Guangzhou 510120, Guangdong, Peoples R China..
    Ding, Ke
    Jinan Univ, Int Cooperat Lab Tradit Chinese Med Modernizat &, Chinese Minist Educ MOE, Sch Pharm,Guangzhou City Key Lab Precis Chem Drug, Guangzhou 510632, Guangdong, Peoples R China.;Guangzhou Med Univ, State Key Lab Resp Dis, Natl Clin Res Ctr Resp Dis, Guangzhou Inst Resp Hlth,Affiliated Hosp 1, Guangzhou 510120, Guangdong, Peoples R China..
    Zhao, Xin
    Guangzhou Med Univ, Key Lab Mol Target & Clin Pharmacol, State Key Lab Resp Dis, Sch Pharmaceut Sci, Guangzhou 511436, Guangdong, Peoples R China.;Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou 511436, Guangdong, Peoples R China.;Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong 999077, Peoples R China..
    Targeted Treatments for Chronic Obstructive Pulmonary Disease (COPD) Using Low-Molecular-Weight Drugs (LMWDs)2019In: Journal of Medicinal Chemistry, ISSN 0022-2623, E-ISSN 1520-4804, Vol. 62, no 13, p. 5944-5978Article in journal (Refereed)
    Abstract [en]

    Chronic obstructive pulmonary disease (COPD) is a very common and frequently fatal airway disease. Current therapies for COPD depend mainly on long-acting bronchodilators, which cannot target the pathogenic mechanisms of chronic inflammation in COPD. New pharmaceutical therapies for the inflammatory processes of COPD are urgently needed. Several anti-inflammatory targets have been identified based on increased understanding of the pathogenesis of COPD, which raises new hopes for targeted treatment of this fatal respiratory disease. In this review, we discuss the recent advances in bioactive low-molecular-weight drugs (LMWDs) for the treatment of COPD and, in addition to the first-line drug bronchodilators, focus particularly on low-molecular-weight anti-inflammatory agents, including modulators of inflammatory mediators, inflammasome inhibitors, protease inhibitors, antioxidants, PDE4 inhibitors, kinase inhibitors, and other agents. We also provide new insights into targeted COPD treatments using LMWDs, particularly small-molecule agents.

  • 8. Zeng, S.
    et al.
    Dou, W.
    Li, M.
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Guo, J.
    Zhao, N.
    Huang, H.
    Zhou, Q.
    Hu, W.
    Ma, Y.
    Zhao, X.
    Xie, H.
    Discovery of an Orally Active and Long-Acting DPP-IV Inhibitor through Property-Based Optimization with an in Silico Biotransformation Prediction Tool2020In: ChemMedChem, ISSN 1860-7179, E-ISSN 1860-7187, Vol. 15, no 16, p. 1608-1617Article in journal (Refereed)
    Abstract [en]

    Long-acting dipeptidyl peptidase IV inhibitors have emerged as promising molecules for interventions for type 2 diabetes. Once weekly dosing brings greater patient compliance and more stable glycemic control. Starting from our previous highly potent compound with a thienoprimidine scaffold, which is unfortunately severely hit by hepatic biotransformation, a lead compound was rapidly generated by drawing on the experience of our previously discovered long-acting compounds with pyrrolopyrimidine scaffold. With the aid of an in silico biotransformation prediction tool, (R)-2-((2-(3-aminopiperidin-1-yl)-4-oxo-6-(pyridin-3-yl)thieno[3,2-d]pyrimidin-3(4H)-yl)methyl)-4-fluorobenzonitrile was eventually generated and determined to have high potency, a fine pharmacokinetic profile, and a long-acting in vivo efficacy.

  • 9.
    Zhou, Hongpo
    et al.
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China.;Kunming Univ Sci & Technol, Fac Chem Engn & Technol, Kunming 650224, Yunnan, Peoples R China..
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Xu, Juan
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Liu, Lanxiang
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Ma, Jinju
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Zhang, Wenwen
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Li, Kun
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Zhang, Hong
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Li, Kai
    Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China..
    Tannic Acid-A Universal Immobilization and Fixation Agent for Nanocarbon Materials: A Novel Strategy for Aqueous Fabrication of Functional Nanocarbon Coating onto Silicon-Based Substances2019In: ACS Sustainable Chemistry and Engineering, E-ISSN 2168-0485, Vol. 7, no 22, p. 18534-18541Article in journal (Refereed)
    Abstract [en]

    We report a feasible and universal approach to fabricate nanocarbon material (NCM) coatings onto a wide range of silicon-based substances. Benefitting from the phenolic hydroxyl and star-shaped branched molecular structure, tannic acid (TA) could act as a functional agent for immobilization and fixation of NCMs in the aqueous phase. More specifically, steered molecular dynamics simulations verified that some unionized chains of TA could tightly attach to the NCMs surface in the neutral aqueous environment by pi-pi stacking interactions, and other free arms with ionized phenolic hydroxyl could act as surface charges of the TA/NCM coacervates, resulting in enhanced colloidal stability of this dispersion. Subsequently, these free arms could also interact with the aminated silicon-based substances, enabling TA to act as a "bridge" between the NCM and the silicon-based substances to form the nanocarbon coating. The obtained polydimethylsiloxane with TA/NCM coating represents favorable electrical and thermal conductivity along with excellent electromechanical performance under the cyclic compression-release test. This strategy is a great improvement for the fabrication of NCM layers and devices, which is not necessary to make the hydrophilic modification of NCM and beneficial to enhance the interaction between the carbon layer and substrate, avoiding the coating separation from the substances.

  • 10.
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Modeling Kinetics of Protein-Ligand Systems2020Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Protein-ligand interactions dominate many life activities and are crucial for thedevelopment of tracers for diagnosing diseases and drugs for treating diseases.For protein-ligand interactions, the binding affinity is conventionally believedto be the most important indicator. However, there is increasing evidencethat the binding affinity alone is not sufficient for providing comprehensiveinformation about protein-ligand interactions. Kinetics, which describes theduration of the interactions and is closely related to the interaction mechanism,is considered as important as, or even more important than, the binding affinityin the study of the mechanisms of protein-ligand interactions.Although kinetics parameters of a protein-ligand system can be measuredexperimentally, the underlying molecular mechanism for the kinetics is difficultto reveal by experiment, which is, however, essential for understanding theorigin of the kinetics and for the rational design of drugs or tracers. In the lastdecade, computer simulations have emerged as a powerful tool for studying biomolecularsystems. Computer simulation methods have also been developedfor modeling kinetics of protein-ligand systems.In this thesis, I explored computer simulations for modeling kinetics propertiesof four different protein-ligand systems. In paper I, I studied the relationshipbetween the ligand binding and conformational changes of the ATAD2-BRD protein. In paper II, I investigated the free energy profile for the coupledfolding and binding of the intrinsically disordered protein p53 with MDM2and calculated the rate constants for the binding and unbinding processes. Inpaper III, I revealed the unbinding paths of the PET tracer ASEM from the  a7-nAChR, calculated the unbinding rate, and explored a way of how to findthe key protein conformational changes strongly coupled to the ligand unbindingprocess. In paper IV, I further refined our methodology for finding theunbinding paths and clarified the unbinding mechanism of the metabolite ofraloxifene from the enzyme CYP3A4.

  • 11.
    Zhou, Yang
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Theoretical Chemistry and Biology. Jinan Univ, Sch Pharm, Guangzhou 510632, Peoples R China..
    Li, Junhao
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Theoretical Chemistry and Biology.
    Baryshnikov, Glib
    Linköping Univ, Dept Sci & Technol, Lab Organ Elect, S-60174 Norrköping, Sweden..
    Tu, Yaoquan
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Theoretical Chemistry and Biology.
    Unraveling the Abnormal Molecular Mechanism of Suicide Inhibition of Cytochrome P450 3A42022In: Journal of Chemical Information and Modeling, ISSN 1549-9596, E-ISSN 1549-960X, Vol. 62, no 23, p. 6172-6181Article in journal (Refereed)
    Abstract [en]

    Suicide inhibition of the CYP3A4 enzyme by a drug inactivates the enzyme in the drug biotransformation process and often shows safety concerns about the drug. Despite extensive experimental studies, the abnormal molecular mechanism of a suicide inhibitor that forms a covalent bond with the residue far away from the catalytically active center of CYP3A4 inactivating the enzyme remains elusive. Here, the authors used molecular simulation approaches to study in detail how diquinone methide (DQR), the metabolite product of raloxifene, unbinds from CYP3A4 and inactivates the enzyme at the atomistic level. The results dearly indicate that in one of the intermediate states formed in its unbinding process, DQR covalently binds to Cys239, a residue far away from the catalytically active center of CYP3A4, and hinders the substrate from entering or leaving the enzyme. This work therefore provides an unprecedented way of clarifying the abnormal mechanism of suicide inhibition of the CYP3A4 enzyme.

  • 12.
    Zhou, Yang
    et al.
    KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
    Nordmark, Arne
    KTH, School of Engineering Sciences (SCI), Mechanics, Structural Mechanics.
    Eriksson, Anders
    KTH, School of Engineering Sciences (SCI), Mechanics, Structural Mechanics.
    Multi-parametric stability investigation for thin spherical membranes with contacts2017In: International Journal of Mechanical Sciences, ISSN 0020-7403, E-ISSN 1879-2162, Vol. 131-132, p. 334-344Article in journal (Refereed)
    Abstract [en]

    The instability behavior for a thin truncated spherical membrane completely filled with fluid or containing both gas and fluid, fixed on a circular platform and in contact with two vertical planes was investigated. Different penalty functions for contacts, and symmetry aspects of the discretized model were studied, and gave effects on instability behavior. Stability conclusions for the multi-parametric problems were made using generalized eigenvalue analyses, showing limit points, bifurcation points and turning point. Contact conditions were shown to introduce bifurcations and secondary paths, dependent on the contact implementations and discretizations. Their effects on stability behaviors in connection with various controlling equations are discussed.

  • 13.
    Zhou, Yang
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology. KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Zou, Rongfeng
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Guanglin, Kuang
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Angstrom, Bengt
    Uppsala Univ, Dept Chem, S-75123 Uppsala, Sweden..
    Halidin, Christer
    Karolinska Inst, Ctr Psychiat Res, Dept Clin Neurosci, S-17176 Stockholm, Sweden.;Stockholm Cty Council, S-17176 Stockholm, Sweden..
    Ågren, Hans
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology. Henan Univ, Coll Chem & Chem Engn, Kaifeng 475004, Henan, Peoples R China..
    Tu, Yaoquan
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Enhanced Sampling Simulations of Ligand Unbinding Kinetics Controlled by Protein Conformational Changes2019In: Journal of Chemical Information and Modeling, ISSN 1549-9596, E-ISSN 1549-960X, Vol. 59, no 9, p. 3910-3918Article in journal (Refereed)
    Abstract [en]

    Understanding unbinding kinetics of protein-ligand systems is of great importance for the design of ligands with desired specificity and safety. In recent years, enhanced sampling techniques have emerged as effective tools for studying unbinding kinetics of protein-ligand systems at the atomistic level. However, in many protein-ligand systems, the ligand unbinding processes are strongly coupled to protein conformational changes and the disclosure of the hidden degrees of freedom closely related to the protein conformational changes so that sampling is enhanced over these degrees of freedom remains a great challenge. Here, we show how potential-scaled molecular dynamics (sMD) and infrequent metadynamics (InMetaD) simulation techniques can be combined to successfully reveal the unbinding mechanism of 3-(1,4-diazabicyclo[3.2.2]nonan-4-yl)-6-[F-18]fluorodibenzo[b,d]thiophen e 5,5-dioxide ([F-18]ASEM) from a chimera structure of the alpha 7-nicotinic acetylcholine receptor. By using sMD simulations, we disclosed that the "close to "open" conformational change of loop C plays a key role in the ASEM unbinding process. By carrying out InMetaD simulations with this conformational change taken into account as an additional collective variable, we further captured the key states in the unbinding process and clarified the unbinding mechanism of ASEM from the protein. Our work indicates that combining sMD and InMetaD simulation techniques can be an effective approach for revealing the unbinding mechanism of a protein-ligand system where protein conformational changes control the unbinding process.

  • 14.
    Zou, Rongfeng
    et al.
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Zhou, Yang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Wang, Yong
    Univ Copenhagen, Linderstrom Lang Ctr Prot Sci, Dept Biol, Struct Biol & NMR Lab, DK-2200 Copenhagen N, Denmark..
    Guanglin, Kuang
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Ågren, Hans
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology.
    Wu, Junchen
    East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China.;East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China..
    Tu, Yaoquan
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Theoretical Chemistry and Biology. Henan Univ, Coll Chem & Chem Engn, Kaifeng 475004, Henan, Peoples R China..
    Free Energy Profile and Kinetics of Coupled Folding and Binding of the Intrinsically Disordered Protein p53 with MDM22020In: Journal of Chemical Information and Modeling, ISSN 1549-9596, E-ISSN 1549-960X, Vol. 60, no 3, p. 1551-1558Article in journal (Refereed)
    Abstract [en]

    Intrinsically disordered proteins (IDPs) exert their functions by binding to partner proteins via a complex process that includes coupled folding and binding. Because inhibiting the binding of the IDP p53 to its partner MDM2 has become a promising strategy for the design of anticancer drugs, we carried out metadynamics simulations to study the coupled folding and binding process linking the IDP p53 to MDM2 in atomic detail. Using bias-exchange metadynamics (BE-MetaD) and infrequent metadynamics (InMetaD), we estimated the binding free energy, the unbinding rate, and the binding rate. By analyzing the stable intermediates, we uncovered the role non-native interactions played in the p53-MDM2 binding/unbinding process. We used a three-state model to describe the whole binding/unbinding process and to obtain the corresponding rate constants. Our work shows that the binding of p53 favors an induced-fit mechanism which proceeds in a stepwise fashion. Our results can be helpful for gaining an in-depth understanding of the coupled folding and binding process needed for the design of MDM2 inhibitors.

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