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  • 1.
    Al-Khalili Szigyarto, Cristina
    et al.
    KTH, Superseded Departments (pre-2005), Biotechnology.
    Persson, A.
    KTH.
    Berglund, L.
    KTH.
    Tourle, S.
    KTH. Royal Inst Technol, Stockholm, Sweden..
    Ekstrom, M.
    KTH.
    Lindskog, M.
    KTH.
    Uhlén, Mathias
    KTH, Superseded Departments (pre-2005), Biotechnology.
    High throughput approach for bioinformatic design and cloning of protein epitope sequence tags suitable for antibody generation2005In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 4, no 8, p. S60-S60Article in journal (Other academic)
  • 2.
    Tegel, Hanna
    et al.
    KTH, School of Biotechnology (BIO), Proteomics.
    Steen, Johanna
    KTH, School of Biotechnology (BIO), Proteomics.
    Konrad, Anna
    KTH, School of Biotechnology (BIO), Proteomics.
    Nikidin, Hero
    KTH, School of Biotechnology (BIO), Proteomics.
    Pettersson, Katarina
    KTH, School of Biotechnology (BIO), Proteomics.
    Stenvall, Maria
    KTH, School of Biotechnology (BIO), Proteomics.
    Tourle, Samuel
    KTH, School of Biotechnology (BIO), Proteomics.
    Wrethagen, Ulla
    KTH, School of Biotechnology (BIO), Proteomics.
    Xu, Lan Lan
    KTH, School of Biotechnology (BIO), Proteomics.
    Yderland, Louise
    KTH, School of Biotechnology (BIO), Proteomics.
    Uhlén, Mathias
    KTH, School of Biotechnology (BIO), Proteomics.
    Hober, Sophia
    KTH, School of Biotechnology (BIO), Proteomics.
    Ottosson, Jenny
    KTH, School of Biotechnology (BIO), Proteomics.
    High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week2009In: Biotechnology Journal, ISSN 1860-6768, E-ISSN 1860-7314, Vol. 4, no 1, p. 51-57Article in journal (Refereed)
    Abstract [en]

    The demand for high-throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for han- dling of nearly 300 different proteins per week. The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated.

  • 3.
    Tegel, Hanna
    et al.
    KTH, School of Biotechnology (BIO), Proteomics.
    Tourle, Samuel
    KTH, School of Biotechnology (BIO), Proteomics.
    Ottosson, Jenny
    KTH, School of Biotechnology (BIO), Proteomics.
    Persson, Anja
    KTH, School of Biotechnology (BIO), Proteomics.
    Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3)2010In: Protein Expression and Purification, ISSN 1046-5928, E-ISSN 1096-0279, Vol. 69, no 2, p. 159-167Article in journal (Refereed)
    Abstract [en]

    The effect of two Escherichia coli expression strains on the production of recombinant human protein fragments was evaluated. High-throughput protein production projects, such as the Swedish Human Protein Atlas project, are dependent on high protein yield and purity. By changing strain from E. coli BL21(DE3) to E. coli Rosetta(DE3) the overall success rate of the protein production has increased dramatically. The Rosetta(DE3) strain compensates for a number of rare codons. Here, we describe how the protein expression of human gene fragments in E. coli strains BL21(DE3) and Rosetta(DE3) was evaluated in two stages. Initially a test set of 68 recombinant proteins that previously had been expressed in BL21(DE3) was retransformed and expressed in Rosetta(DE3). The test set generated very positive results with an improved expression yield and a significantly better purity of the protein product which prompted us to implement the Rosetta(DE3) strain in the high-throughput protein production. Except for analysis of protein yield and purity the sequences were also analyzed regarding number of rare codons and rare codon clusters. The content of rare codons showed to have a significant effect on the protein purity. Based on the results of this study the atlas project permanently changed expression strain to Rosetta(DE3).

  • 4.
    Tourle, S.
    et al.
    KTH.
    Tegel, Hanna
    KTH.
    Ottosson, J.
    KTH.
    Persson, A.
    KTH.
    Increased levels of recombinant human proteins in E-Coli Rosetta that compensates for mammalian codon usage2006In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 5, no 10, p. S223-S223Article in journal (Other academic)
  • 5.
    Uhlén, Mathias
    et al.
    KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
    Björling, Erik
    KTH, School of Biotechnology (BIO).
    Agaton, Charlotta
    KTH, School of Biotechnology (BIO).
    Al-Khalili Szigyarto, Cristina
    KTH, School of Biotechnology (BIO).
    Amini, Bahram
    KTH, School of Biotechnology (BIO).
    Andersen, Elisabet
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Andersson, Ann-Catrin
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Angelidou, Pia
    KTH, School of Biotechnology (BIO).
    Asplund, Anna
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Asplund, Caroline
    KTH, School of Biotechnology (BIO).
    Berglund, Lisa
    KTH, School of Biotechnology (BIO).
    Bergström, Kristina
    KTH, School of Biotechnology (BIO).
    Brumer, Harry
    KTH, School of Biotechnology (BIO).
    Cerjan, Dijana
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Ekström, Marica
    KTH, School of Biotechnology (BIO).
    Elobeid, Adila
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Eriksson, Cecilia
    KTH, School of Biotechnology (BIO).
    Fagerberg, Linn
    KTH, School of Biotechnology (BIO).
    Falk, Ronny
    KTH, School of Biotechnology (BIO).
    Fall, Jenny
    KTH, School of Biotechnology (BIO).
    Forsberg, Mattias
    KTH, School of Biotechnology (BIO).
    Gry Björklund, Marcus
    KTH, School of Biotechnology (BIO).
    Gumbel, Kristoffer
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Halimi, Asif
    KTH, School of Biotechnology (BIO).
    Hallin, Inga
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Hamsten, Carl
    KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
    Hansson, Marianne
    KTH, School of Biotechnology (BIO).
    Hedhammar, My
    KTH, School of Biotechnology (BIO).
    Hercules, Görel
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Kampf, Caroline
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Larsson, Karin
    KTH, School of Biotechnology (BIO).
    Lindskog, Mats
    KTH, School of Biotechnology (BIO).
    Lodewyckx, Wald
    KTH, School of Biotechnology (BIO).
    Lund, Jan
    KTH, School of Biotechnology (BIO).
    Lundeberg, Joakim
    KTH, School of Biotechnology (BIO).
    Magnusson, Kristina
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Malm, Erik
    KTH, School of Biotechnology (BIO).
    Nilsson, Peter
    KTH, School of Biotechnology (BIO).
    Ödling, Jenny
    KTH, School of Biotechnology (BIO).
    Oksvold, Per
    KTH, School of Biotechnology (BIO).
    Olsson, Ingmarie
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Öster, Emma
    KTH, School of Biotechnology (BIO).
    Ottosson, Jenny
    KTH, School of Biotechnology (BIO).
    Paavilainen, Linda
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Persson, Anja
    KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
    Rimini, Rebecca
    KTH, School of Biotechnology (BIO).
    Rockberg, Johan
    KTH, School of Biotechnology (BIO).
    Runeson, Marcus
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Sivertsson, Åsa
    KTH, School of Biotechnology (BIO).
    Sköllermo, Anna
    KTH, School of Biotechnology (BIO).
    Steen, Johanna
    KTH, School of Biotechnology (BIO).
    Stenvall, Maria
    KTH, School of Biotechnology (BIO).
    Sterky, Fredrik
    KTH, School of Biotechnology (BIO).
    Strömberg, Sara
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Sundberg, Mårten
    KTH, School of Biotechnology (BIO).
    Tegel, Hanna
    KTH, School of Biotechnology (BIO).
    Tourle, Samuel
    KTH, School of Biotechnology (BIO).
    Wahlund, Eva
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Waldén, Annelie
    KTH, School of Biotechnology (BIO).
    Wan, Jinghong
    KTH, School of Biotechnology (BIO), Molecular Biotechnology (closed 20130101).
    Wernérus, Henrik
    KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
    Westberg, Joakim
    KTH, School of Biotechnology (BIO).
    Wester, Kenneth
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    Wrethagen, Ulla
    KTH, School of Biotechnology (BIO).
    Xu, Lan Lan
    KTH, School of Biotechnology (BIO).
    Hober, Sophia
    KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
    Pontén, Fredrik
    Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol.
    A human protein atlas for normal and cancer tissues based on antibody proteomics2005In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 4, no 12, p. 1920-1932Article in journal (Refereed)
    Abstract [en]

    Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.

1 - 5 of 5
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  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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  • html
  • text
  • asciidoc
  • rtf