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  • 1.
    Chen, Fei
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Monnier, Xavier
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Gällstedt, Mikael
    Innventia, Sweden.
    Gedde, Ulf W.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Wheat gluten/chitosan blends: A new biobased material2014Ingår i: European Polymer Journal, ISSN 0014-3057, E-ISSN 1873-1945, Vol. 60, s. 186-197Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Wheat gluten and chitosan are renewable materials that suffer from some poor properties that limit their use as a potential replacement of petroleum-based polymers. However, polymer blends based on wheat gluten and chitosan surprisingly reduced these shortcomings. Films were cast from acidic aqueous or water/ethanol solutions of wheat gluten and chitosan. Wheat gluten was the discontinuous phase in the 30-70 wt.% wheat gluten interval investigated. The most homogeneous films were obtained when reducing agents were used (alone or together with urea or glycerol). They consisted mainly of 1-2 mu m wheat gluten particles uniformly distributed in the continuous chitosan phase. Slightly smaller particles were also observed in the water/ethanol solvent system, but together with significantly larger particles (as large as 200 mu m). Both small and large particles were observed, albeit in different sizes and contents, when surfactants (both with and without a reducing agent) or urea (without a reducing agent) were used. The particles were often elongated, and preferably along the film, the most extreme case being observed when the glyoxal crosslinker was used together with sodium sulfite (reducing agent), showing particles with an average thickness of 0.6 mu m and an aspect ratio of 4.2. This film showed the highest transparency of all the blend films studied. For one of the most promising systems (with sodium sulfite), having good film homogeneity and small particles, the mechanical and moisture solubility/diffusivity properties were studied as a function of chitosan content. The extensibility, toughness and moisture solubility increased with increasing chitosan content, and the moisture diffusivity was highest for the pristine chitosan material. It is noteworthy that the addition of 30 wt.% wheat gluten to chitosan reduced the moisture uptake, while the extensibility/toughness remained unchanged.

  • 2.
    Cho, Sung-Woo
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknologi, Polymera material.
    Gällstedt, Mikael
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknologi, Polymera material.
    Effects of glycerol content and film thickness on the properties of vital wheat gluten films cast at pH 4 and 12010Ingår i: Journal of Applied Polymer Science, ISSN 0021-8995, E-ISSN 1097-4628, Vol. 117, nr 6, s. 3506-3514Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This study deals with the optical properties and plasticizer migration properties of vital wheat gluten (WG) films cast at pH 4 and 11. The films contained initially 8, 16, and 25 wt % glycerol and were aged at 23 degrees C and 50% relative humidity for at least 17 weeks on a paper support to simulate a situation where a paper packaging is laminated with an oxygen barrier film of WG. The films, having target thicknesses of 50 and 250 mu m, were characterized visually and with ultraviolet/visible and infrared spectroscopy; the mass loss was measured by gravimetry or by a glycerol-specific gas chromatography method. The thin films produced at pH 4 were, in general, more heterogeneous than those produced at pH 11. The thin pH 4 films consisted of transparent regions surrounding beige glycerol-rich regions, the former probably rich in gliadin and the latter rich in glutenin. This, together with less Maillard browning, meant that the thin pH 4 films, in contrast to the more homogeneous (beige) thin pH 11 films, showed good contact clarity. The variations in glycerol content did not significantly change the optical properties of the films. All the films showed a significant loss of glycerol to the paper support but, after almost 9 months, the thick pH 11 film containing initially 25 wt % glycerol was still very flexible and, despite a better contact to the paper, had a higher residual glycerol content than the pH 4 film, which was also more brittle.

  • 3.
    Cho, Sung-Woo
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Gällstedt, Mikael
    KTH.
    Hedenqvist, Mikael S
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Properties of Wheat Gluten/Poly(lactic acid) Laminates2010Ingår i: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 58, nr 12, s. 7344-7350Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Laminates of compression-molded glycerol-plasticized wheat gluten (WG) films surrounded and supported by poly(lactic acid) (PLA) films have been produced and characterized. The objective was to obtain a fully renewable high gas barrier film with sufficient mechanical integrity to function in, for example, extrusion-coating paper/board applications. It was shown that the lamination made it possible to make films with a broad range of glycerol contents (0-30 wt %) with greater strength than single unsupported WG films. The low plasticizer contents yielded laminates with very good oxygen barrier properties. In addition, whereas the unsupported WO films had an immeasurably high water vapor transmission rate (WVTR), the laminate showed values that were finite and surprisingly, in several cases, also lower than that of PLA. Besides being a mechanical support (as evidenced by bending and tensile data) and a shield between the WG and surrounding moisture, the PLA layer also prevented the loss of the glycerol plasticizer from the WG layer. This was observed after the laminate had been aged on an "absorbing" blotting paper for up to 17 weeks. The interlayer adhesion (peel strength) decreased with decreasing glycerol content and increasing WG film molding temperature (130 degrees C instead of 110 degrees C). The latter effect was probably due to a higher protein aggregation, as revealed by infrared spectroscopy. The lamination temperature (110-140 degrees C) did not, however, have a major effect on the final peel strength.

  • 4.
    Cho, Sung-Woo
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Ullsten, Henrik
    STFI Packforsk AB.
    Gällstedt, Mikael
    STFI Packforsk AB.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Heat-sealing properties of compression-molded wheat gluten films2007Ingår i: Journal of Biobased Materials and Bioenergy, ISSN 1556-6560, Vol. 1, nr 1, s. 56-63Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The impulse heat-sealing properties of wheat gluten films were investigated. Films containing 30 wt% glycerol were compression molded at 100-130 degrees C and then sealed in a lap-shear or peel-test geometry at 120-175 degrees C. The tensile properties of the pristine films and the lap-shear and peel strength of the sealed films were evaluated and the seals were examined by scanning electron microscopy. Glycerol was added to the film surfaces prior to sealing in an attempt to enhance the seal strength. It was observed that the wheat gluten films were readily sealable. At a 120 degrees C sealing temperature and without glycerol as adhesive, the lap-shear strength was greater than or similar to that of polyethylene film, although the peel strength was poorer. The sealing temperature had a negligible effect on the lap-shear strength, but the peel strength increased with sealing temperature. The lap-shear strength increased with increasing mold temperature and the failure mode changed, especially in the absence of glycerol adhesive, from a cohesive (material failure) to an adhesive type. From previous results, it is known that the high-temperature (130 degrees C) compression-molded film was highly cross-linked and aggregated, and this prevents molecular interdiffusion and entanglement and thus leads to incomplete seal fusion and, in general, adhesive failure. The presence of glycerol adhesive had a beneficial affect on the peel strength but no, or only a minor, effect on the lap-shear strength.

  • 5.
    Gällstedt, Mikael
    KTH, Tidigare Institutioner, Fiber- och polymerteknologi.
    Films and composites based on chitosan, wheat gluten or whey proteins -Their packaging related mechanical and barrier properties2004Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
  • 6. Gällstedt, Mikael
    et al.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Türe, Hasan
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Production, Chemistry and Properties of Proteins2011Ingår i: Biopolymers: New Materials for Sustainable Films and Coatings / [ed] David Plackett, Chichester: John Wiley & Sons, 2011, s. 107-132Kapitel i bok, del av antologi (Övrigt vetenskapligt)
  • 7. Johansson, K. S.
    et al.
    Chen, H.
    Hofmann, J. L.
    Swerin, A.
    Hedenqvist, Mikael
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Dubreuil, M.
    Vangeneugden, D.
    Gällstedt, Mikael
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Surface modification of wheat gluten films for improved water resistance2010Ingår i: ACS National Meeting Book of Abstracts, 2010Konferensbidrag (Refereegranskat)
    Abstract [en]

    Renewable packaging materials are of interest for a more sustainable environment, and wheat gluten (WG) is one of the most interesting candidates to replace petroleum-based oxygen-barrier polymers for packaging applications. This is due to its attractive combination of flexibility and strength, high gas (especially O 2) barrier properties under low humidity conditions and renewability. The main drawback of WG, as with most biopolymers, is its water and moisture sensitivity. The aim of this study was therefore to improve the hydrophobicity of WG films by means of surface modification while maintaining the excellent O 2 barrier properties. The surface modification work included a combination of electrospinning of WG fibers and different plasma surface modifications. The latter involved He plasma treatment for crosslinking the WG film prior to the deposition of electrospun WG fibers, O 2/Ar plasma etching of the WG films with and without electrospun WG fibers for increasing the surface roughness, and plasma polymerization of hexamethyldisiloxane (HMDSO) and other hydrophobic precursors for hydrophobicity. The plasma polymerization trials were performed both at reduced and atmospheric pressure conditions. The aim of the combined work was to maximize the hydrophobicity by combining a suitable nano-microstructure of the WG fibers with the hydrophobicity of the plasma-deposited coatings. The surface modification work was mainly evaluated by means of water contact angle measurements (hydrophobicity), Scanning Electron Microscopy (surface structures), Water Vapor Transmission Rate (WVTR) (moisture barrier) and Oxygen Transmission Rate (OTR) measurements (oxygen barrier). The surface modification work resulted in significantly improved hydrophobic properties of the WG films. The initial water contact angle increased from 65 to 110-130 degrees, depending on the combinations of electrospinning and plasma modification conditions. The plasma coatings prepared at ambient conditions resulted in slightly lower contact angles compared the plasma coating prepared at reduced pressure. The WVTR and OTR measurements are still in progress and will be reported at the meeting.

  • 8.
    Olabarrieta, Idoia
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Cho, Sung Woo
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Gällstedt, Mikael
    STFI-Packforsk.
    Sarasua, Jose-Ramon
    Engineering Faculty, University of Basque Country.
    Johansson, Eva
    Department of Crop Science, The Swedish University of Agricultural Sciences.
    Hedenqvist, Mikael S
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Aging properties of films of plasticized vital wheat gluten cast from acidic and basic solutions2006Ingår i: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 7, nr 5, s. 1657-1664Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In order to understand the mechanisms behind the undesired aging of films based on vital wheat gluten plasticized with glycerol, films cast from water/ethanol solutions were investigated. The effect of pH was studied by casting from solutions at pH 4 and pH 11. The films were aged for 120 days at 50% relative humidity and 23 C, and the tensile properties and oxygen and water vapor permeabilities were measured as a function of aging time. The changes in the protein structure were determined by infrared spectroscopy and size-exclusion and reverse-phase high-performance liquid chromatography, and the film structure was revealed by optical and scanning electron microscopy. The pH 11 film was mechanically more stable with time than the pH 4 film, the latter being initially very ductile but turning brittle toward the end of the aging period. The protein solubility and infrared spectroscopy measurements indicated that the protein structure of the pH 4 film was initially significantly less polymerized/ aggregated than that of the pH 11 film. The polymerization of the pH 4 film increased during storage but it did not reach the degree of aggregation of the pH 11 film. Reverse-phase chromatography indicated that the pH 11 films were to some extent deamidated and that this increased with aging. At the same time a large fraction of the aged pH 11 film was unaffected by reducing agents, suggesting that a time-induced isopeptide cross-linking had occurred. This isopeptide formation did not, however, change the overall degree of aggregation and consequently the mechanical properties of the film. During aging, the pH 4 films lost more mass than the pH 11 films mainly due to migration of glycerol but also due to some loss of volatile mass. Scanning electron and optical microscopy showed that the pH 11 film was more uniform in thickness and that the film structure was more homogeneous than that of the pH 4 film. The oxygen permeability was also lower for the pH 11 film. The fact that the pH 4 film experienced a larger and more rapid change in its mechanical properties with time than the pH 11 film, as a consequence of a greater loss of plasticizer, was presumably due to its initial lower degree of protein aggregation/ polymerization. Consequently, the cross-link density achieved at pH 4 was too low to effectively retain volatiles and glycerol within the matrix.

  • 9.
    Olabarrieta, Idoia
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Gällstedt, Mikael
    Ispizua, Iban
    Sarasua, Jose-Ramon
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Properties of new and aged montmorillonite-wheat gluten composite films2006Ingår i: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 54, nr 4, s. 1283-1288Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The properties of new and aged glycerol-plasticized vital wheat gluten films containing ≤4.5 wt % natural or quaternary ammonium salt modified montmorillonite clay were investigated. The films were cast from pH 4 or pH 11 ethanol/water solutions. The films, aged for ≤120 days, were characterized by tensile testing, X-ray diffraction, and transmission electron microscopy. In addition, water vapor permeability (11% relative humidity) and the content of volatile components were measured. The large reduction in the water vapor permeability with respect to the pristine polymer suggests that the clay platelets were evenly distributed within the films and oriented preferably with the platelet long axis parallel to the film surface. The film prepared from pH 11 solution containing natural clay was, as revealed by transmission electron microscopy and X-ray diffraction, almost completely exfoliated. This film was consequently also the strongest, the stiffest, and the most brittle and, together with the pH 11 film containing modified clay, it also showed the greatest decrease in water vapor permeability. The large blocking effect of the clay had no effect on the aging kinetics of the films. During aging, the pH 4 and pH 11 film strength and the pH 4 film stiffness increased and the pH 4 film ductility decreased at the same rate with or without clay. This suggests that the aging was not diffusion rate limited, that is, that the loss of volatile components or the migration of glycerol or glycerol/wheat gluten phase separation was not limited by diffusion kinetics. The aging rate seemed to be determined by slow structural changes, possibly involving protein denaturation and aggregation processes.

  • 10.
    Ture, Hasan
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Blomfeldt, Tomas O. J.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Gällstedt, Mikael
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Farris, Stefano
    University of Milan.
    Nanostructured Silica Wheat Gluten Hybrid Materials Prepared by Catalytic Sol-Gel Chemistry2013Ingår i: Macromolecular Chemistry and Physics, ISSN 1022-1352, E-ISSN 1521-3935, Vol. 214, nr 10, s. 1131-1139Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The main physicochemical properties of nanostructured silica/wheat gluten hybrid composites are presented. The extraction experiments suggest that the protein phase is intimately encased within the silica matrix, with silica–protein interactions driven by hydrogen bonding, as indicated by IR spectra. Spectroscopic results also show that silica induces a higher degree of constraint of the wheat gluten matrix, despite less aggregation. Moisture diffusion properties of the hybrid materials are investigated by a combined “desorption/sorption” approach. While the reduction of the moisture diffusivity in the presence of silica can be described by the geometrical impedance of a “sintered” porous solid, a time-dependent relaxation/restructuring of the composite apparently occurs during the sorption-desorption cycle.

  • 11.
    Türe, Hasan
    et al.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Blomfeldt, Thomas
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Gällstedt, Mikael
    Innventia AB.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik, Polymera material.
    Properties of Wheat-Gluten/Montmorillonite Nanocomposite Films Obtained by a Solvent-Free Extrusion Process2012Ingår i: Journal of polymers and the environment, ISSN 1064-7546, E-ISSN 1572-8900, Vol. 20, nr 4, s. 1038-1045Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This is, to our knowledge, the first study of wheat-gluten-based nanocomposite films prepared by a solvent-free extrusion process. Wheat gluten/montmorillonite nanocomposite films were obtained in a single screw-extruder using urea as a combined denaturant and plasticizer. The oxygen permeability and water vapor transmission rate of the films decreased by respectively factors of 1. 9 and 1. 3 when 5 wt.% clay was added. At the same time, the stiffness increased by a factor of 1. 5, without any critical loss of extensibility. Field emission scanning electron microscopy (FE-SEM) and Energy-dispersive X-ray analysis indicated that the clay particles were layered mainly in the plane of the extruded film. It was possible to identify individual platelets/tactoids with FE-SEM and, together with findings from transmission electron microscopy, atomic force microscopy and X-ray diffraction, it was concluded that the clay existed as individual clay platelets, intercalated tactoids and agglomerates. Thermogravimetric analysis showed that the thermal stability of the extrudates was improved by the addition of clay.

  • 12.
    Ullsten, Henrik
    et al.
    STFI-Packforsk.
    Cho, Sung-Woo
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Spencer, Gwen
    SLU, Alnarp.
    Gällstedt, Mikael
    STFI-Packforsk.
    Johansson, Eva
    SLU, Alnarp.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Properties of Extruded Vital Wheat Gluten Sheets with Sodium Hydroxide and Salicylic Acid2009Ingår i: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 10, nr 3, s. 479-488Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This paper presents a novel approach to improve the barrier and mechanical properties of extruded glycerol-plasticized vital wheat gluten sheets. The sheets were extruded with a single screw extruder at alkaline conditions using 3-5 wt % NaOH. Salicylic acid (SA), known to improve the extrudability of wheat gluten, was also added alone or in combination with NaOH. Oxygen transmission rate and volatile mass measurements, tensile tests, protein solubility, glycerol migration, infrared spectroscopy, and electrophoresis were used to assess the properties of the extrudate. Electrophoresis showed that the gluten/glycerol sheet and the sheet with 3 wt % NaOH and I wt % SA contained the same building blocks in terms of proteins and protein subunits, although the protein solubility in these samples was different. The oxygen barrier, at dry conditions, was improved significantly with the addition of NaOH, On the other hand, the addition of salicylic acid yielded poorer barrier properties. The extrudate was placed on a blotting paper and its aging properties were investigated during the first 120 days. It was observed that the extrudate with 3 wt % NaOH had the most suitable combination of properties (low oxygen permeability, large strain at break, and relatively small aging-induced changes in mechanical properties); the reason is probably due to low plasticizer migration and an optimal protein aggregation/polymerization.

  • 13. Ullsten, Henrik
    et al.
    Gällstedt, Mikael
    Johansson, Eva Lena
    Gräslund, Astrid
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Enlarged processing window of plasticized wheat gluten using salicylic acid2006Ingår i: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 7, nr 3, s. 771-776Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The temperature window for the extrusion of glycerol-plasticized wheat gluten was increased by the use of salicylic acid, a known scorch retarder and radical scavenger. It was possible to extrude 30 wt % glycerol-wheat gluten films with a die-head temperature as high as 135 °C, rather than 95 °C, by incorporating only 1 wt % salicylic acid. Small effects of shear-induced heating during extrusion at the higher temperatures suggested that the acid acted as a lubricant and viscosity reducer. The latter was suggested to originate primarily from the salicylic-acid-induced reduction in the degree of protein aggregation/cross-linking, as indicated by size-exclusion high-performance liquid chromatography and chemiluminescence. Electron paramagnetic resonance spectroscopy on extruded films indicated that the beneficial effect of salicylic acid was due to its radical scavenging effect. Tensile tests on extrudates revealed that the materials produced at the substantially higher processing temperature were still ductile. The complex shear modulus increased more slowly with increasing salicylic acid content above 110-120 °C, indicating that the aggregation/cross-linking rate was slower with salicylic acid, th t is, that it did have a scorch-retarding effect, besides yielding a lower final degree/complexity of aggregation.

  • 14. Ullsten, Henrik
    et al.
    Gällstedt, Mikael
    Spencer, G. M.
    Johansson, E.
    Marttila, S.
    Ignell, R.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Extrusion of wheat gluten with ammonium hydroxideManuskript (Övrigt vetenskapligt)
  • 15. Ullsten, Henrik
    et al.
    Hedenqvist, Mikael S.
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknik.
    Gällstedt, Mikael
    Plasticizers for thermoformed wheat gluten filmsManuskript (Övrigt vetenskapligt)
1 - 15 av 15
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