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  • 1.
    Pei, Yuxin
    et al.
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Yu, Hui
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Pei, Zhichao
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Theurer, Matthias
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Ammer, Carolin
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    André, Sabine
    Gabius, Hans-Joachim
    Yan, Mingdi
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Ramström, Olof
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Photoderivatized polymer thin films at quartz crystal microbalance surfaces: Sensors for carbohydrate-protein interactions2007In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, no 18, p. 6897-6902Article in journal (Refereed)
    Abstract [en]

    Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.

  • 2.
    Pei, Zhichao
    et al.
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Pei, Yuxin
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Yu, Hui
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Theurer, Matthias
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    André, Sabine
    Gabius, Hans-Joachim
    Yan, Mingdi
    Ramström, Olof
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Photoderivatized QCM Surfaces for the Study of Real-Time Lectin-Carbohydrate InteractionsArticle in journal (Refereed)
  • 3.
    Pei, Zhichao
    et al.
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Yu, Hui
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Theurer, Matthias
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Waldén, Annelie
    KTH, School of Biotechnology (BIO).
    Nilsson, Peter
    KTH, School of Biotechnology (BIO).
    Yan, Mingdi
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Ramström, Olof
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Photogenerated Carbohydrate Microarrays2007In: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 8, no 2, p. 166-168Article in journal (Refereed)
    Abstract [en]

    Chemical Equation Presented) Sugars in a row. A new strategy for carbohydrate microarrays based on photochemical ligation of perfluorophenylazide-derivatized carbohydrates to PEO surfaces is presented. It constitutes a controllable and robust method of array fabrication, on the carbohydrate chemistry and on the surface-chemistry levels, and the resulting carbohydrate arrays can be efficiently used to reveal the recognition patterns of carbohydrate-binding proteins.

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