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  • 1.
    Pei, Yuxin
    et al.
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Yu, Hui
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Pei, Zhichao
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Theurer, Matthias
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Ammer, Carolin
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    André, Sabine
    Gabius, Hans-Joachim
    Yan, Mingdi
    KTH, School of Chemical Science and Engineering (CHE), Chemistry.
    Ramström, Olof
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
    Photoderivatized polymer thin films at quartz crystal microbalance surfaces: Sensors for carbohydrate-protein interactions2007In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, no 18, p. 6897-6902Article in journal (Refereed)
    Abstract [en]

    Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.

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