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  • 1. Bettencourt, R.
    et al.
    Assefaw-Redda, Yohannes
    Faye, I.
    The insect immune protein hemolin is expressed during oogenesis and embryogenesis2000In: Mechanisms of Development, ISSN 0925-4773, E-ISSN 1872-6356, Vol. 95, no 02-jan, p. 301-304Article in journal (Refereed)
    Abstract [en]

    Hemolin is the most abundant bacteria-induced proteins in Hyalophora cecropia hemolymph. Its structural features, both at the protein and gene level, ascribe this molecule to the immunoglobulin gene superfamily (IgSF) with particular homology to neural cell adhesion molecules. An increasing number of evidence suggest a role in immune recognition and in cell adhesion events. Hemolin is also developmentally regulated as suggested by changes in its concentration during larval and pupal ecdysis (Trenczek, T., 1998. Endogenous defense mechanisms of insects. Zoology 101, 298-315; Lanz-Mendoza, H., Faye, I., 1999. Physiological aspects of the immunoglobulin superfamily in invertebrates. Dev. Comp. Immunol. 23, 359-374). In the present study the expression of hemolin was investigated in oogenesis and in early embryogenesis. Our results reveal that hemolin is expressed in follicles and in epidermal and neural tissues of embryos.

  • 2. Lanner, Johanna T.
    et al.
    Bruton, Joseph D.
    Assefaw-Redda, Yohannes
    KTH, School of Information and Communication Technology (ICT), Integrated Devices and Circuits.
    Andronache, Zoita
    Severa, Denise
    Zhang, Zhibin
    KTH, School of Information and Communication Technology (ICT), Integrated Devices and Circuits.
    Melzer, Werner
    Zhang, Shi-Li
    KTH, School of Information and Communication Technology (ICT), Integrated Devices and Circuits.
    Katz, Abram
    Westerblad, Hakan
    Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells2009In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 23, no 6, p. 1728-1738Article in journal (Refereed)
    Abstract [en]

    The involvement of Ca2+ in the insulin-mediated signaling cascade, resulting in glucose uptake in skeletal muscle, is uncertain. Here, we test the hypothesis that Ca2+ influx through canonical transient receptor potential 3 (TRPC3) channels modulates insulin-mediated glucose uptake in adult skeletal muscle. Experiments were performed on adult skeletal muscle cells of wild-type (WT) and obese, insulin-resistant ob/ob mice. Application of the diacylglycerol analog 1-oleyl-2-acetyl-sn-glycerol (OAG) induced a nonselective cation current, which was inhibited by the addition of anti-TRPC3 antibody in the patch pipette and smaller in ob/ob than in WT cells. Knockdown of TRPC3, using a novel technique based on small interfering RNA (siRNA) coupled to functionalized carbon nanotubes, resulted in pronounced (similar to 70%) decreases in OAG-induced Ca2+ influx and insulin-mediated glucose uptake. TRPC3 and the insulin-sensitive glucose transporter 4 (GLUT4) coimmunoprecipitated, and immunofluorescence staining showed that they were colocalized in the proximity of the transverse tubular system, which is the predominant site of insulin-mediated glucose transport in skeletal muscle. In conclusion, our results indicate that TRPC3 interacts functionally and physically with GLUT4, and Ca2+ influx through TRPC3 modulates insulin-mediated glucose uptake. Thus, TRPC3 is a potential target for treatment of insulin-resistant conditions.-Lanner, J. T., Bruton, J. D., Assefaw-Redda, Y., Andronache, Z., Zhang, S.- J., Severa, D., Zhang, Z.- B., Melzer, W., Zhang, S.-L., Katz, A., Westerblad, H. Knockdown of TRPC3 with siRNA coupled to carbon nanotubes results in decreased insulin-mediated glucose uptake in adult skeletal muscle cells. FASEB J. 23, 1728-1738 (2009)

  • 3. Roxstrom-Lindquist, K.
    et al.
    Assefaw-Redda, Yohannes
    Rosinska, K.
    Faye, I.
    20-Hydroxyecdysone indirectly regulates Hemolin gene expression in Hyalophora cecropia2005In: Insect molecular biology (Print), ISSN 0962-1075, E-ISSN 1365-2583, Vol. 14, no 6, p. 645-652Article in journal (Refereed)
    Abstract [en]

    Development and innate immune defence are two vital processes that have been demonstrated to use the same or similar molecules and signalling pathways in insects. Hemolin is a moth haemolymph protein belonging to the immunoglobulin superfamily. It is strongly induced upon bacterial infection. However, recent studies indicate a developmental regulation of hemolin. We show that the steroid hormone 20-hydroxyecdysone (20E) can activate the expression of Hyalophora cecropia Hemolin (HcHemolin) in the fat body of diapausing pupae. Using the protein synthesis inhibitor cycloheximide we demonstrate that Hemolin up-regulation by 20E requires ongoing protein synthesis. Moreover, 20E enhances transcription of the Hemolin gene in response to bacteria. Comparing the upstream regions of Manduca sexta Hemolin (MsHemolin) and HcHemolin, we identified four putative regulatory sites. Two are putative hormone response elements (HREs), one with an imperfect inverted repeat (HRE-IR) and one with a monomeric site (HRE-M). An additional monomeric hormone receptor site (MRE) is present only in HcHemolin. The third conserved motif is similar to the interferon (IFN) regulatory factor binding element (IRF-E) and IFN-stimulated response element (ISRE). The fourth conserved element is a kappa B motif situated between the Cap-site and the TATA-box. Finally, by electrophoresis mobility shift assay we demonstrate that the HRE-IR forms specific complexes with nuclear extract proteins of normal pupae that increase after 20E stimulation.

  • 4.
    Wang, Yong-Bin
    et al.
    KTH, School of Information and Communication Technology (ICT), Integrated Devices and Circuits.
    Assefaw-Redda, Yohannes
    KTH, School of Biotechnology (BIO).
    Gabig-Ciminska, Magdalena
    KTH, School of Biotechnology (BIO), Bioprocess Technology.
    Enfors, Sven-Olof
    KTH, School of Biotechnology (BIO), Bioprocess Technology.
    Östling, Mikael
    KTH, School of Information and Communication Technology (ICT), Microelectronics and Information Technology, IMIT.
    Zhang, Shi-Li
    KTH, School of Information and Communication Technology (ICT), Microelectronics and Information Technology, IMIT.
    A novel dual mode capacitor biosensor for real-time, label-free DNA detection2006In: 2006 INTERNATIONAL ELECTRON DEVICES MEETING, VOLS 1 AND 2, NEW YORK: IEEE , 2006, p. 447-450Conference paper (Refereed)
    Abstract [en]

    A novel biosensor working in both MOS capacitor and electric double-layer capacitor mode for real-time, label-free DNA detection is presented. The former mode measures density and polarity of charged biomolecules to be monitored, while the latter mode appears to reveal more of surface-molecule interactions and offers high sensitivity and easiness for diagnosis. A combined mode-utilization yields optimal complementary information of significance.

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