kth.sePublikationer
Ändra sökning
Avgränsa sökresultatet
123 1 - 50 av 106
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Träffar per sida
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
  • Standard (Relevans)
  • Författare A-Ö
  • Författare Ö-A
  • Titel A-Ö
  • Titel Ö-A
  • Publikationstyp A-Ö
  • Publikationstyp Ö-A
  • Äldst först
  • Nyast först
  • Skapad (Äldst först)
  • Skapad (Nyast först)
  • Senast uppdaterad (Äldst först)
  • Senast uppdaterad (Nyast först)
  • Disputationsdatum (tidigaste först)
  • Disputationsdatum (senaste först)
Markera
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 1.
    Ayoglu, Burcu
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Affinity Arrays for Profiling Proteins and Autoantibody Repertoires2014Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Ladda ner fulltext (pdf)
    thesis
  • 2.
    Bai, Yunpeng
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Weibull, Emilie
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Jönsson, Håkan
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Andersson Svahn, Helene
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Interfacing picoliter droplet microfluidics with addressable microliter compartments using fluorescence activated cell sorting2014Ingår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 194, s. 249-254Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Droplet microfluidic platforms have, while enabling high-throughput manipulations and the assaying of single cell scale compartments, been lacking interfacing to allow macro scale access to the output from droplet microfluidic operations. Here, we present a simple and high-throughput method for individually directing cell containing droplets to an addressable and macro scale accessible microwell slide for downstream analysis. Picoliter aqueous droplets containing low gelling point agarose and eGFP expressing Escherichia coli (E. coli) are created in a microfluidic device, solidified to agarose beads and transferred into an aqueous buffer. A Fluorescence activated cell sorter (FACS) is used to sort agarose beads containing cells into microwells in which the growth and expansion of cell colonies is monitored. We demonstrate fast sorting and high accuracy positioning of sorted 15 μm gelled droplet agarose beads into microwells (14 × 48) on a 25 mm × 75 mm microscope slide format using a FACS with a 100 μm nozzle and an xy-stage. The interfacing method presented here enables the products of high-throughput or single cell scale droplet microfluidics assays to be output to a wide range of microtiter plate formats familiar to biological researchers lowering the barriers for utilization of these microfluidic platforms.

  • 3. Bao, D.
    et al.
    Zou, Zhuo
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Industriell och Medicinsk Elektronik. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    Huan, Y.
    Zhai, Chuanying
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Industriell och Medicinsk Elektronik. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    Bagaian, T.
    Tenhunen, Hannu
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Industriell och Medicinsk Elektronik. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    Källbäck, B.
    Zheng, Lirong
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Industriell och Medicinsk Elektronik. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK. State Key Laboratory of ASIC and System, Fudan University, Shanghai, China .
    A smart catheter system for minimally invasive brain monitoring2015Ingår i: Proceedings of the International Conference on Biomedical Electronics and Devices, SciTePress, 2015, s. 198-203Konferensbidrag (Refereegranskat)
    Abstract [en]

    This paper demonstrates a smart catheter system with intracranial pressure (ICP) and temperature sensing capability which is designed for real-time monitoring in traumatic brain injury (TBI) therapy. It uses a single flexible catheter with a 1 mm (3 Fr) diameter that integrates electrodes and sophisticated silicon chip on flexible substrates, enabling multimodality monitoring of physiological signals. A micro-electromechanical-system (MEMS) catheter pressure sensor is mounted on the distal end. It can be used for detecting both pressure and temperature by different switch configurations, which minimizes the size of catheter and reduces the cost. The interconnects (signalling conductors) are printed on a bio-compatible flexible substrate, and the sensor is interfaced with an embedded electronic system at the far-end. The electronic system consists of analog front end with analog-to-digital converter (ADC), a microcontroller, and data interface to the hospital infrastructure with a graphical user interface (GUI). The overall smart catheter system achieves a pressure sensing root mean square error (RMSE) of ±1.5 mmHg measured from 20 mmHg to 300 mmHg above 1 atm and a temperature sensing RMSE of ±0.08°C measured from 32°C to 42°C. The sampling rate can be up to 10S/s. The in vivo performance is demonstrated in laboratory animals.

  • 4.
    Bauer, Margit
    et al.
    Med Univ Graz, Dept Obstet & Gynecol, Graz, Austria..
    Mazza, Edoardo
    Swiss Fed Inst Technol, Dept Mech & Proc Engn, Zurich, Switzerland..
    Jabareen, Mahmood
    Swiss Fed Inst Technol, Dept Mech & Proc Engn, Zurich, Switzerland..
    Sultan, Leila
    Univ Zurich Hosp, Dept Obstet & Gynecol, CH-8091 Zurich, Switzerland..
    Bajka, Michael
    Univ Zurich Hosp, Dept Obstet & Gynecol, CH-8091 Zurich, Switzerland..
    Lang, Uwe
    Med Univ Graz, Dept Obstet & Gynecol, Graz, Austria..
    Zimmermann, Roland
    Univ Zurich Hosp, Dept Obstet & Gynecol, CH-8091 Zurich, Switzerland..
    Holzapfel, Gerhard A.
    KTH, Skolan för teknikvetenskap (SCI).
    In Vivo Biomechanical Testing of the Human Uterine Cervix in Pregnancy Using an Aspiration Device2009Ingår i: Reproductive Sciences, ISSN 1933-7191, E-ISSN 1933-7205, Vol. 16, nr 3, s. 197A-197AArtikel i tidskrift (Övrigt vetenskapligt)
  • 5.
    Bjällmark, Anna
    KTH, Skolan för teknik och hälsa (STH), Medicinsk teknik.
    New ultrasonographic approaches to monitoring cardiac and vascular function2009Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Atherosclerotic cardiovascular disease is the leading cause of death worldwide. To decrease mortality and morbidity in cardiovascular disease, the development of accurate, non-invasive methods for early diagnosis of atherosclerotic cardiac and vascular engagement is of considerable clinical interest. Cardiovascular ultrasound imaging is today the cornerstone in the routine evaluation of cardiovascular function and recent development has resulted in two new techniques, tissue velocity imaging (TVI) and speckle tracking, which allow objective quantification of cardiovascular function. TVI and speckle tracking are the basis for three new approaches to cardiac and vascular monitoring presented in this thesis: wave intensity wall analysis (WIWA), two-dimensional strain imaging in the common carotid artery, and the state diagram of the heart.

     

    WIWA uses longitudinal and radial strain rate as input for calculations of wave intensity in the arterial wall. In this thesis, WIWA was validated against a commercially available wave intensity system, showing that speckle tracking-derived strain variables can be useful in wave intensity analysis. WIWA was further tested in patients with end stage renal disease and documented high mortality in cardiovascular disease. The latter study evaluated the effects of a single session of hemodialysis using WIWA and TVI variables and showed improved systolic function after hemodialysis. The results also indicated that preload-adjusted early systolic wave intensity obtained by the WIWA system may contribute in the assessment of left ventricular contractility in this patient category. Two-dimensional strain imaging in the common carotid artery is a new approach showing great potential to detect age-dependent differences in mechanical properties of the common carotid artery. Among the measured strain variables, global circumferential strain had the best discriminating performance and appeared to be superior to conventional measures of arterial stiffness such as elastic modulus and β stiffness index. The state diagram is a visualisation tool that provides a quantitative overview of the temporal interrelationship of mechanical events in the left and right ventricles. Case examples and a small clinical study showed that state diagrams clearly visualize cardiac function and can be useful in the detection of non ST-elevation myocardial infarction (NSTEMI).

     

    Even though WIWA, two-dimensional strain imaging in the common carotid artery and the state diagram show potential to be useful in the evaluation of cardiovascular function, there still remains a considerable amount of work to be done before they can be used in the daily clinical practice.

    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 6.
    Boltshauser, Rasmus
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Medicinteknik och hälsosystem.
    Development of a Novel Device for Optimal Sample Blood Volume Collection from Patients with Sepsis2020Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    When performing sepsis diagnosis, the most important preanalytical variable is blood volume. Too little blood increases the risk for false negatives whereas overfilling causes increased risk for false positives. Even though this fact is known, there are case studies showing that in a majority of tests, the taken blood sample volume is not the recommended amount. As previously tried methods have been limited in their ability to tackle the problem this study aimed at creating a technical device to aid healthcare providers with blood volume sample collection. As a base, the double diamond approach by the Design Council was used. This design approach splits up the design process in four distinctly different phases (discover, define, develop, and deliver) all using their own methods to aid the creative process.

    After completing the discover and define phase it was determined that a non-contact capacitance liquid level sensor could operate as an ideal blood volume sample device. During the development and delivery phase prototypes were created and evaluated. The final results of this work could not give conclusive evidence concerning if a non-contact liquid level sensor could operate as an ideal blood volume collection device. The methodological approach used in this thesis can be used as inspiration for a designer to create a device for a similar or different purpose. Moreover, information from this thesis can also work as reference material to develop a device to perform ideal blood volume sample collection.

    Such a device would have the potential to be an essential part of the everyday workflow in sample collection from patients with sepsis worldwide and would aid in ensures effective and fast diagnostics.

    Ladda ner fulltext (pdf)
    fulltext
  • 7. Bora, Kangkana
    et al.
    Chowdhury, Manish
    KTH, Skolan för teknik och hälsa (STH).
    Mahanta, Lipi B.
    Kundu, Malay Kumar
    Das, Anup Kumar
    Automated classification of Pap smear images to detect cervical dysplasia2017Ingår i: Computer Methods and Programs in Biomedicine, ISSN 0169-2607, E-ISSN 1872-7565, Vol. 138, s. 31-47Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background and objectives: The present study proposes an intelligent system for automatic categorization of Pap smear images to detect cervical dysplasia, which has been an open problem ongoing for last five decades. Methods: The classification technique is based on shape, texture and color features. It classifies the cervical dysplasia into two-level (normal and abnormal) and three-level (Negative for Intraepithelial Lesion or Malignancy, Low-grade Squamous Intraepithelial Lesion and High-grade Squamous Intraepithelial Lesion) classes reflecting the established Bethesda system of classification used for diagnosis of cancerous or precancerous lesion of cervix. The system is evaluated on two generated databases obtained from two diagnostic centers, one containing 1610 single cervical cells and the other 1320 complete smear level images. The main objective of this database generation is to categorize the images according to the Bethesda system of classification both of which require lots of training and expertise. The system is also trained and tested on the benchmark Herlev University database which is publicly available. In this contribution a new segmentation technique has also been proposed for extracting shape features. Ripplet Type I transform, Histogram first order statistics and Gray Level Co-occurrence Matrix have been used for color and texture features respectively. To improve classification results, ensemble method is used, which integrates the decision of three classifiers. Assessments are performed using 5 fold cross validation. Results: Extended experiments reveal that the proposed system can successfully classify Pap smear images performing significantly better when compared with other existing methods. Conclusion: This type of automated cancer classifier will be of particular help in early detection of cancer.

  • 8. Bose, Indranil
    et al.
    Ohlander, Anna
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Kutter, Christoph
    Russom, Aman
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi.
    DNA Analysis on integrated all foil based microdevicesManuskript (preprint) (Övrigt vetenskapligt)
  • 9.
    Bottier, Mathieu
    et al.
    Eq. 13, Institut Mondor de Recherche Biomédicale, Inserm U955, Créteil, France.
    Peña Fernández, Marta
    Zeiss Global Centre, School of Mechanical and Design Engineering, University of Portsmouth, Portsmouth, PO1 3DJ, UK.
    Pelle, Gabriel
    Eq. 13, Institut Mondor de Recherche Biomédicale, Inserm U955, Créteil, France.
    Isabey, Daniel
    Eq. 13, Institut Mondor de Recherche Biomédicale, Inserm U955, Créteil, France.
    Louis, Bruno
    Eq. 13, Institut Mondor de Recherche Biomédicale, Inserm U955, Créteil, France.
    Grotberg, James B
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA..
    Filoche, Marcel
    Eq. 13, Institut Mondor de Recherche Biomédicale, Inserm U955, Créteil, France.
    A new index for characterizing micro-bead motion in a flow induced by ciliary beating: Part II, modeling.2017Ingår i: PloS Computational Biology, ISSN 1553-734X, E-ISSN 1553-7358, Vol. 13, nr 7, artikel-id e1005552Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Mucociliary clearance is one of the major lines of defense of the human respiratory system. The mucus layer coating the airways is constantly moved along and out of the lung by the activity of motile cilia, expelling at the same time particles trapped in it. The efficiency of the cilia motion can experimentally be assessed by measuring the velocity of micro-beads traveling through the fluid surrounding the cilia. Here we present a mathematical model of the fluid flow and of the micro-beads motion. The coordinated movement of the ciliated edge is represented as a continuous envelope imposing a periodic moving velocity boundary condition on the surrounding fluid. Vanishing velocity and vanishing shear stress boundary conditions are applied to the fluid at a finite distance above the ciliated edge. The flow field is expanded in powers of the amplitude of the individual cilium movement. It is found that the continuous component of the horizontal velocity at the ciliated edge generates a 2D fluid velocity field with a parabolic profile in the vertical direction, in agreement with the experimental measurements. Conversely, we show than this model can be used to extract microscopic properties of the cilia motion by extrapolating the micro-bead velocity measurement at the ciliated edge. Finally, we derive from these measurements a scalar index providing a direct assessment of the cilia beating efficiency. This index can easily be measured in patients without any modification of the current clinical procedures.

  • 10.
    Cakici, Baki
    et al.
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Kommunikation: Infrastruktur och tjänster, Programvaru- och datorsystem, SCS.
    Hebing, Kenneth
    Swedish Institute for Infectious Control (SMI), Solna, Sweden.
    Grünewald, Maria
    Swedish Institute for Infectious Control (SMI), Solna, Sweden.
    Saretok, Paul
    Swedish Institute for Infectious Control (SMI), Solna, Sweden.
    Hulth, Anette
    Swedish Institute for Infectious Control (SMI), Solna, Sweden.
    CASE: a framework for computer supported outbreak detection2010Ingår i: BMC Medical Informatics and Decision Making, E-ISSN 1472-6947, Vol. 10, s. 14-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: In computer supported outbreak detection, a statistical method is applied to a collection of cases to detect any excess cases for a particular disease. Whether a detected aberration is a true outbreak is decided by a human expert. We present a technical framework designed and implemented at the Swedish Institute for Infectious Disease Control for computer supported outbreak detection, where a database of case reports for a large number of infectious diseases can be processed using one or more statistical methods selected by the user. Results: Based on case information, such as diagnosis and date, different statistical algorithms for detecting outbreaks can be applied, both on the disease level and the subtype level. The parameter settings for the algorithms can be configured independently for different diagnoses using the provided graphical interface. Input generators and output parsers are also provided for all supported algorithms. If an outbreak signal is detected, an email notification is sent to the persons listed as receivers for that particular disease. Conclusions: The framework is available as open source software, licensed under GNU General Public License Version 3. By making the code open source, we wish to encourage others to contribute to the future development of computer supported outbreak detection systems, and in particular to the development of the CASE framework.

  • 11.
    Cavallaro, Sara
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Fotonik.
    Development of Techniques for Characterization, Detection and Protein Profiling of Extracellular Vesicles2021Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Nanosized extracellular vesicles (EVs, ∼30-2000 nm) have emerged as important mediators of intercellular communication, offering opportunities for both diagnostics and therapeutics. In particular, small EVs generated from the endolysosomal pathway (∼30-150 nm), referred to as exosomes, have attracted interest as a suitable biomarker for cancer diagnostics and treatment monitoring based on minimally invasive liquid biopsies. This is because exosomes carry valuable biological information (proteins, lipids, genetic material, etc.) reflecting their cells of origin. Using EVs as biomarkers or drug delivery agents in clinical applications requires a full understanding of their cellular origin, functions, and biological relevance. However, due to their small size and very high heterogeneity in molecular and physical features, the analysis of these vesicles is challenged by the limited detection ranges and/or accuracy of the currently available techniques. To overcome some of these challenges, this thesis focuses on developing different techniques for characterization, detection and protein profiling of EVs at both bulk and single particle levels. Specifically, the three methods investigated are scanning electron microscopy, electrokinetic sensing, and combined fluorescence - atomic force microscopy. 

    First, a protocol for scanning electron microscopy imaging of EVs was optimized to improve the throughput and image quality of the method while preserving the shape of the vesicles. Application of the developed protocol for analysis of EVs from human serum showed the possibility to use scanning electron microscopy for morphological analysis and high-resolution size-based profiling of EVs over their entire size range. Comparison with nanoparticle tracking analysis, a commonly used technique for EV size estimation, showed a superior sensitivity of scanning electron microscopy for particles smaller than 70-80 nm. Moreover, the study showed process steps that can generate artifacts resembling sEVs and ways to minimize them. 

    Secondly, a novel label-free electrokinetic sensor based on streaming current was developed, optimized and multiplexed for EV protein analysis at a bulk level. Using multiple microcapillary sensors functionalized with antibodies, the method showed the capacity for multiplexed detection of different surface markers on small EVs from non-small-cell lung cancer cells. The device performance in the multichannel configuration remained similar to the single-channel one in terms of noise, detection sensitivity, and reproducibility. The application of the technique for analysis of EVs isolated from lung cancer patients with different genomic alterations and after different applied treatments demonstrated the prospect of using EVs from liquid biopsies as a source of biomarker for cancer monitoring. Moreover, the results held promise for the application of the developed method in clinical settings. 

    Finally, to increase the understanding of EV subpopulations and heterogeneity, a platform combining fluorescence and atomic force microscopy was developed for multiparametric analysis of EVs at a single particle level. The use of a precise spot identification approach and an efficient vesicle capture protocol allowed to study and correlate for the first time the membrane protein composition, size and mechanical properties (Young modulus) on individual small EVs. The application of the technique to vesicles isolated from different cell lines identified both common and cell line-specific EV subpopulations bearing distinct distributions of the analyzed parameters. For example, a sEV population co-expressing all the three analyzed proteins in relatively high abundance, yet having average diameters of <100 nm and relatively low Young moduli was found in all cell lines. The obtained results highlighted the possibility of using the developed platform to help decipher unsolved questions regarding EV biology. 

    Ladda ner fulltext (pdf)
    summary
  • 12.
    Cheng, Xiaogang
    et al.
    KTH, Skolan för elektroteknik och datavetenskap (EECS). Nanjing Univ Posts & Telecommun, Coll Telecommun & Informat Engn, Nanjing 210003, Jiangsu, Peoples R China.;Umea Univ, Dept Appl Phys & Elect, S-90187 Umea, Sweden.
    Yang, Bin
    Umea Univ, Dept Appl Phys & Elect, S-90187 Umea, Sweden.;Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Shaanxi, Peoples R China..
    Liu, Guoqing
    Nanjing Tech Univ, Sch Phys & Math Sci, Nanjing 211800, Jiangsu, Peoples R China..
    Olofsson, Thomas
    Umea Univ, Dept Appl Phys & Elect, S-90187 Umea, Sweden..
    Li, Haibo
    KTH, Skolan för elektroteknik och datavetenskap (EECS). Nanjing Univ Posts & Telecommun, Coll Telecommun & Informat Engn, Nanjing 210003, Jiangsu, Peoples R China..
    A Total Bounded Variation Approach to Low Visibility Estimation on Expressways2018Ingår i: Sensors, E-ISSN 1424-8220, Vol. 18, nr 2, artikel-id 392Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Low visibility on expressways caused by heavy fog and haze is a main reason for traffic accidents. Real-time estimation of atmospheric visibility is an effective way to reduce traffic accident rates. With the development of computer technology, estimating atmospheric visibility via computer vision becomes a research focus. However, the estimation accuracy should be enhanced since fog and haze are complex and time-varying. In this paper, a total bounded variation (TBV) approach to estimate low visibility (less than 300 m) is introduced. Surveillance images of fog and haze are processed as blurred images (pse udo-blurred images), while the surveillance images at selected road points on sunny days are handled as clear images, when considering fog and haze as noise superimposed on the clear images. By combining image spectrum and TBV, the features of foggy and hazy images can be extracted. The extraction results are compared with features of images on sunny days. Firstly, the low visibility surveillance images can be filtered out according to spectrum features of foggy and hazy images. For foggy and hazy images with visibility less than 300 m, the high-frequency coefficient ratio of Fourier (discrete cosine) transform is less than 20%, while the low-frequency coefficient ratio is between 100% and 120%. Secondly, the relationship between TBV and real visibility is established based on machine learning and piecewise stationary time series analysis. The established piecewise function can be used for visibility estimation. Finally, the visibility estimation approach proposed is validated based on real surveillance video data. The validation results are compared with the results of image contrast model. Besides, the big video data are collected from the Tongqi expressway, Jiangsu, China. A total of 1,782,000 frames were used and the relative errors of the approach proposed are less than 10%.

  • 13.
    Chinnasamy, Thiruppathiraja
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Segerink, Loes I.
    Nystrand, Mats
    Gantelius, Jesper
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Andersson Svahn, Helene
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    A lateral flow paper microarray for rapid allergy point of care diagnostics2014Ingår i: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 139, nr 10, s. 2348-2354Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    There is a growing need for multiplexed specific IgE tests that can accurately evaluate patient sensitization profiles. However, currently available commercial tests are either single/low-plexed or require sophisticated instrumentation at considerable cost per assay. Here, we present a novel convenient lateral flow microarray-based device that employs a novel dual labelled gold nanoparticle-strategy for rapid and sensitive detection of a panel of 15 specific IgE responses in 35 clinical serum samples. Each gold nanoparticle was conjugated to an optimized ratio of HRP and anti-IgE, allowing significant enzymatic amplification to improve the sensitivity of the assay as compared to commercially available detection reagents. The mean inter-assay variability of the developed LFM assay was 12% CV, and analysis of a cohort of clinical samples (n = 35) revealed good general agreement with ImmunoCAP, yet with a varying performance among allergens (AUC = [0.54-0.88], threshold 1 kU). Due to the rapid and simple procedure, inexpensive materials and read-out by means of a consumer flatbed scanner, the presented assay may provide an interesting low-cost alternative to existing multiplexed methods when thresholds > 1 kU are acceptable.

  • 14.
    de Sousa, Nuno Rufino
    et al.
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden..
    Sandström, Niklas
    KTH, Centra, Science for Life Laboratory, SciLifeLab. Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
    Shen, Lei
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden.;Tongji Univ, Shanghai Pulm Hosp, Sch Med, Shanghai, Peoples R China..
    Hakansson, Kathleen
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden..
    Vezozzo, Rafaella
    Fed Univ Grande Dourados, Fac Hlth Sci, Dourados, MS, Brazil..
    Udekwu, Klas, I
    Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.;SoS BIOS, Uppsala, Sweden..
    Croda, Julio
    Univ Fed Mato Grosso do Sul, Sch Med, Campo Grande, MS, Brazil.;Fundacao Oswaldo Cruz, Campo Grande, MS, Brazil..
    Rothfuchs, Antonio Gigliotti
    Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden..
    A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols2020Ingår i: Tuberculosis, ISSN 1472-9792, E-ISSN 1873-281X, Vol. 120, artikel-id 101896Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Tuberculosis (TB) infects about 25% of the world population and claims more human lives than any other infectious disease. TB is spread by inhalation of aerosols containing viable Mycobacterium tuberculosis expectorated or exhaled by patients with active pulmonary disease. Air-sampling technology could play an important role in TB control by enabling the detection of airborne M. tuberculosis, but tools that are easy to use and scalable in TB hotspots are lacking. We developed an electrostatic air sampler termed the TB Hotspot DetectOR (THOR) and investigated its performance in laboratory aerosol experiments and in a prison hotspot of TB transmission. We show that THOR collects aerosols carrying microspheres, Bacillus globigii spores and M. bovis BCG, concentrating these microparticles onto a collector piece designed for subsequent detection analysis. The unit was also successfully operated in the complex setting of a prison hotspot, enabling detection of a molecular signature for M. tuberculosis in the cough of inmates. Future deployment of this device may lead to a measurable impact on TB case-finding by screening individuals through the aerosols they generate.

  • 15.
    Demir Kanik, Sumeyra Ummuhan
    et al.
    Signal Processing Technologies LLC, Richmond, VA, USA.
    Hakimzadeh, Roya
    Hargraves, Rosalyn Hobson
    Ward, Kevin R
    Myer, Eric V
    Najarian, Kayvan
    An automated method for analysis of microcirculation videos for accurate assessment of tissue perfusion2012Ingår i: BMC Medical Imaging, E-ISSN 1471-2342, Vol. 12, s. 1-13Artikel i tidskrift (Refereegranskat)
  • 16.
    Dijkstra, Erik J.
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Mekanik, Biomekanik.
    Gutierrez-Farewik, Elena M.
    KTH, Skolan för teknikvetenskap (SCI), Mekanik, Biomekanik. Karolinska Institutet, Stockholm, Sweden.
    Computation of ground reaction force using Zero Moment Point2015Ingår i: Journal of Biomechanics, ISSN 0021-9290, E-ISSN 1873-2380, Vol. 48, nr 14, s. 3776-3781Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Motion analysis is a common clinical assessment and research tool that uses a camera system or motion sensors and force plates to collect kinematic and kinetic information of a subject performing an activity of interest. The use of force plates can be challenging and sometimes even impossible. Over the past decade, several computational methods have been developed that aim to preclude the use of force plates. Useful in particular for predictive simulations, where a new motion or change in control strategy inherently means different external contact loads. These methods, however, often depend on prior knowledge of common observed ground reaction force (GRF) patterns, are computationally expensive, or difficult to implement. In this study, we evaluated the use of the Zero Moment Point as a computationally inexpensive tool to obtain the GRFs for normal human gait. The method was applied on ten healthy subjects walking in a motion analysis laboratory and predicted GRFs are evaluated against the simultaneously measured force plate data. Apart from the antero-posterior forces, GRFs are well-predicted and errors fall within the error ranges from other published methods. Joint extension moments were underestimated at the ankle and hip but overestimated at the knee, attributable to the observed discrepancy in the predicted application points of the GRFs. The computationally inexpensive method evaluated in this study can reasonably well predict the GRFs for normal human gait without using prior knowledge of common gait kinetics.

  • 17. Downs, J.
    et al.
    Velupillai, Sumithra
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Datavetenskap, Teoretisk datalogi, TCS.
    George, G.
    Holden, R.
    Kikoler, M.
    Dean, H.
    Fernandes, A.
    Dutta, R.
    Detection of Suicidality in Adolescents with Autism Spectrum Disorders: Developing a Natural Language Processing Approach for Use in Electronic Health Records2017Ingår i: AMIA Annual Symposium Proceedings, E-ISSN 1942-597X, Vol. 2017, s. 641-649Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Over 15% of young people with autism spectrum disorders (ASD) will contemplate or attempt suicide during adolescence. Yet, there is limited evidence concerning risk factors for suicidality in childhood ASD. Electronic health records (EHRs) can be used to create retrospective clinical cohort data for large samples of children with ASD. However systems to accurately extract suicidality-related concepts need to be developed so that putative models of suicide risk in ASD can be explored. We present a systematic approach to 1) adapt Natural Language Processing (NLP) solutions to screen with high sensitivity for reference to suicidal constructs in a large clinical ASD EHR corpus (230,465 documents), and 2) evaluate within a screened subset of 500 patients, the performance of an NLP classification tool for positive and negated suicidal mentions within clinical text. When evaluated, the NLP classification tool showed high system performance for positive suicidality with precision, recall, and F1 scores all > 0.85 at a document and patient level. The application therefore provides accurate output for epidemiological research into the factors contributing to the onset and recurrence of suicidality, and potential utility within clinical settings as an automated surveillance or risk prediction tool for specialist ASD services.

  • 18.
    Ekelund, Emil
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Medicinteknik och hälsosystem.
    LactateStat: Wearable Electronics and Software for Real-Time Lactate Monitoring in Sweat2021Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Laktat är en viktig biomarkör inom idrott och konceptet laktattröskel är en av de bästa indikatorerna för uthållighet hos en idrottsutövare. Men för att mäta laktattröskeln krävs en invasiv metod som går ut på att man tar ett blodprov. Begränsningarna med denna metod är bland annat att idrottsutövningen måste stoppas för att ta ett blodprov och att man inte får realtidsåterkoppling.

    I stället kan en liten och icke-invasiv bärbar biosensor användas för att mäta laktatkoncentrationen i svett. Sensorn genererar en elektrisk ström beroende på laktatkoncentrationen i svetten och en enhet som kallas potentiostat måste därför användas för att mäta denna ström. Tillgängliga potentiostater är dock inte lämpliga för användning inom sport där formfaktor, batteritid och trådlös kommunikation är viktigt. Denna avhandling har som syfte att lösa detta genom att utveckla en bärbar enhet och mjukvara som kan användas för att mäta laktatkoncentrationen i svett i realtid under träning.

    Utvecklingsprocessen bestod av bestämning av specifikationer, framtagning av en prototyp och noggrann laboratorie- och kroppstestning.

    Som resultat presenterades en banbrytande bärbar enhet och mjukvara som kan mäta laktat i realtid i svett under träning. Enheten, som kallades LactateStat, var 58mm * 55mm * 13mm, den aktiva strömförbrukningen var 7.8mA, den bästa mätupplösningen för ström var 0.5 nA, detektionsgränsen var 0.45 nA och det maximala mätområdet för ström var ca. 750 μA.

    LactateStat är en av de första i sitt slag och ger en bas för framtida utvecklingsarbete eftersom hårdvaru-, programvaru- och mjukvaruresurserna tillhandahålls som öppen källkod. 

    Ladda ner fulltext (pdf)
    fulltext
  • 19.
    Engblom, Camilla
    et al.
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
    Thrane, Kim
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Genteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Lin, Qirong
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
    Andersson, Alma
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Genteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Toosi, Hosein
    SciLifeLab, Computational Science and Technology department, KTH Royal Institute of Technology, Stockholm, Sweden.
    Chen, Xinsong
    Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
    Mantovani, Giulia
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
    Hagemann-Jensen, Michael
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
    Saarenpää, Sami
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Genteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Hartman, Johan
    Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
    Lagergren, Jens
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Datavetenskap, Beräkningsvetenskap och beräkningsteknik (CST). KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Mold, Jeff
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
    Lundeberg, Joakim
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Genteknologi.
    Frisén, Jonas
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
    Spatial transcriptomics of T and B cell receptors maps lymphocyte clones in human tissueManuskript (preprint) (Övrigt vetenskapligt)
  • 20.
    Engvall, Alice
    et al.
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Medicinteknik och hälsosystem.
    Eriksson-Viklund, Tuva
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Medicinteknik och hälsosystem.
    RNAlater som bevaringsmetod för muskelvävnad: En jämförande metodstudie om frystorkning, RNAlater och RNAlater-ICE som bevaring av skelettmuskulatur inför molekylärbiologiska analyser2022Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    I denna metodstudie jämfördes Thermo Fishers bevarande lösningar RNAlater och RNAlater-ICE med frystorkning som bevaring av skelettmuskulatur från människa. Studien gjordes med syftet att avgöra om RNAlater och/eller RNAlater-ICE kan ersätta frystorkning, i hopp om att underlätta dissekering av muskelvävnad. De molekylärbiologiska analyser som utfördes var proteinbestämning; Western Blot inriktad på proteinerna mTor, S6, S6K1, eEF2, myosin typ II och aktin; samt mätning av glykogen och citratsyntasaktivitet. Därtill påbörjades även en aminosyraanalys. Studien utfördes hos William Apró på Åstrandlaboratoriet på Gymnastik- och idrottshögskolan i Stockholm. Resultaten visade att både RNAlater och RNAlater-ICE är olämpliga att använda i studier som innefattar samtliga av de genomförda analyserna. Detta då analysresultaten från de alternativa bevaringsmetoderna och de från frystorkningen inte var likvärdiga. Därmed drogs slutsatsen att varken RNAlater eller RNAlater-ICE kan ersätta frystorkning som bevaringsmetod i Aprós vidare studier.

    Ladda ner fulltext (pdf)
    fulltext
  • 21. Ericsson, Anders B.
    et al.
    Kronander, Håkan
    Söderqvist, Emil
    Vaage, Jarle
    Brodin, Lars-Åke
    Correlation between a Mid-ventricular Volume Segment and Global Left Ventricular Volume Measured by the Conductance Catheter2001Ingår i: Scandinavian Cardiovascular Journal, ISSN 1401-7431, E-ISSN 1651-2006, Vol. 35, nr 2, s. 129-135Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Objectives-To investigate whether acute volume changes in single volume segments of the left ventricle can be correlated with global volume changes. If so, changes in global volume might be predicted from changes in segmental volumes.

    Design-Volume changes were recorded in six pigs in five intraventricular segments, from apex to heart base, using the conductance catheter (at baseline, after 60 min of apical ischaemia, during preload reduction and afterload increase). A computer algorithm was created to calculate the instantaneous absolute difference between the curve shape of global and normalized segmental volume as a percentage of global stroke volume.

    Results-For a mid-cardiac volume segment constituting 34 (14-39)% [median (range)] of global stroke volume, the mean difference over a cardiac cycle was 4 (1-8)% at baseline. Apical ischaemia resulted in apical dyskinesia, but did not influence the mid-cardiac segment.

    Conclusions-The volume curve from a segment at mid-cardiac level seems to be a good estimator of the global volume curve, thus giving a foundation for estimation of global volume changes from such a segment.

  • 22.
    Eriksson, Magnus G.
    KTH, Skolan för industriell teknik och management (ITM), Maskinkonstruktion (Inst.).
    A virtual and haptic milling surgery simulator2006Rapport (Övrigt vetenskapligt)
  • 23.
    Eriksson, Magnus G.
    KTH, Skolan för teknik och hälsa (STH).
    Haptic and visual simulation of material cutting process: a study focused on bone surgery and the use of simulators for education and training2006Licentiatavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    A prototype of a haptic and virtual reality simulator has been developed for simulation of the bone milling and material removal process occurring in several operations, e.g. temporal bone surgery or dental milling. The milling phase of an operation is difficult, safety critical and very time consuming. Reduction of operation time by only a few percent would in the long run save society large expenses. In order to reduce operation time and to provide surgeons with an invaluable practicing environment, this licentiate thesis discusses the introduction of a simulator system to be used in both surgeon curriculum and in close connection to the actual operations.

    The virtual reality and haptic feedback topics still constitute a young and unexplored area. It has only been active for about 10-15 years for medical applications. High risk training on real patients and the change from open surgery to endoscopic procedures have enforced the introduction of haptic and virtual reality simulators for training of surgeons. Increased computer power and the similarity to the successful aviation simulators also motivate to start using simulators for training of surgical skills.

    The research focus has been twofold: 1) To develop a well working VR-system for realistic graphical representation of the skull itself including the changes resulting from milling, and 2) to find an efficient algorithm for haptic feedback to mimic the milling procedure using the volumetric Computer Tomography (CT) data of the skull. The developed haptic algorithm has been verified and tested in the simulator. The visualization of the milling process is rendered at a graphical frame rate of 30 Hz and the haptic rendering loop is updated at 1000 Hz. Test results show that the real-time demands are fulfilled. The visual and haptic implementations have been the two major steps to reach the over all goal with this research project.

    A survey study is also included where the use of VR and haptic simulators in the surgical curriculum is investigated. The study starts with a historical perspective of the VR and haptic topics and is built up by answering different questions related to this topic and the implementation of simulators at the medical centres. The questions are of general concern for those developing surgical VR and haptic simulators.

    Suggested future work includes modelling, development and validation of the haptic forces occurring in the milling process and, based on this, implementation in the simulator system. Also, further development of the simulator should be done in close cooperation with surgeons in order to get appropriate feedback for further improvements of the functionality and performance of the simulator.

    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 24.
    Eriksson, Magnus G.
    et al.
    KTH, Skolan för industriell teknik och management (ITM), Maskinkonstruktion (Inst.), Mekatronik.
    Dixon, Mark R.
    SenseGraphics.
    Wikander, Jan
    KTH, Skolan för industriell teknik och management (ITM), Maskinkonstruktion (Inst.), Mekatronik.
    A haptic VR milling surgery simulator - using high-resolution CT-data2006Ingår i: Studies in Health Technology and Informatics, ISSN 0926-9630, E-ISSN 1879-8365, Vol. 119, s. 138-143Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A haptic virtual reality milling simulator using high resolution volumetric data is presented in this paper. We discuss the graphical rendering performed from an iso-surface generated using marching cubes with a hierarchical storage method to optimize for fast dynamic changes to the data during the milling process. We also present a stable proxy-based haptic algorithm used to maintain a tip position on the surface avoiding haptic fall-through.

  • 25.
    Eriksson, Magnus G.
    et al.
    KTH, Skolan för industriell teknik och management (ITM), Maskinkonstruktion (Inst.).
    Wikander, Jan
    KTH, Skolan för industriell teknik och management (ITM), Maskinkonstruktion (Inst.).
    von Holst, Hans
    KTH, Skolan för teknik och hälsa (STH), Neuronik.
    The use of virtual reality and haptic simulators for training and education of surgical skills2006Ingår i: Simulation in Healthcare: journal of the society for simulation in healthcare, ISSN 1559-2332Artikel i tidskrift (Övrigt vetenskapligt)
  • 26.
    Etcheverry, Sebastián
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Laserfysik. RISE Acreo AB, Sweden.
    Russom, Aman
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Laurell, Fredrik
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Laserfysik.
    Margulis, Walter
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
    Fluidic trapping and optical detection of microparticles with a functional optical fiber2017Ingår i: Optics Express, E-ISSN 1094-4087, Vol. 25, nr 26, artikel-id 33657Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    A fiber probe is presented that traps single micro-sized particles and allows detection of their optical properties. The trapping mechanism used is based on fluid suction with a micro-structured optical fiber that has five holes along its cladding. Proof-of-principle experiments with a diluted solution of fluorescently labeled particles are performed. The fiber probe presented here may find various applications in life-science and environmental monitoring.  

  • 27.
    Etehad Tavakol, Mahnaz
    et al.
    Isfahan University of Medical Sciences, Iran.
    Fatemi, Alimohammad
    Isfahan University of Medical Sciences, Iran.
    Karbalaie, Abdolamir
    KTH, Skolan för teknik och hälsa (STH), Hälso- och systemvetenskap.
    Emrani, Zahra
    Isfahan University of Medical Sciences, Iran.
    Erlandsson, Björn-Erik
    KTH, Skolan för teknik och hälsa (STH), Hälso- och systemvetenskap, Systemsäkerhet och organisation.
    Nailfold Capillaroscopy in Rheumatic Diseases: Which Parameters Should Be Evaluated?2015Ingår i: BioMed Research International, ISSN 2314-6133, E-ISSN 2314-6141, Vol. 2015, artikel-id 974530Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Video nailfold capillaroscopy (NFC), considered as an extension of the widefield technique, allows a more accurate measuring andstoring of capillary data and a better defining, analyzing, and quantifying of capillary abnormalities. Capillaroscopic study is oftenperformed on the patients suspected of having microcirculation problems such as Raynaud’s phenomenon as the main indicationfor nailfold capillaroscopy. Capillaroscopic findings based on microcirculation studies can provide useful information in the fieldsof pathophysiology, differential diagnosis, and monitoring therapy. Nailfold capillaroscopy provides a vital assessment in clinicalpractices and research; for example, its reputation in the early diagnosis of systemic sclerosis is well established and it is also usedas a classification criterion in this regard. This review focuses on the manner of performing video nailfold capillaroscopy and on acommon approach for measuring capillary dimensions in fingers and toes.

    Ladda ner fulltext (pdf)
    fulltext
  • 28.
    Faridi, Muhammad Asim
    et al.
    KTH.
    Shahzad, Adnan Faqui
    KTH.
    Russom, Aman
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Nanobioteknologi.
    Wiklund, Martin
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
    Milliliter scale acoustophoresis based bioparticle processing platform2018Ingår i: ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, ASME Press, 2018Konferensbidrag (Refereegranskat)
    Abstract [en]

    Bioparticles such as mammalian cells and bacteria can be manipulated directly or indirectly for multiple applications such as sample preparation for diagnostic applications mainly up-concentration, enrichment & separation as well as immunoassay development. There are various active and passive microfluidic particle manipulation techniques where Acoustophoresis is a powerful technique showing high cell viability. The use of disposable glass capillaries for acoustophoresis, instead of cleanroom fabricated glass-silicon chip can potentially bring down the cost factor substantially, aiding the realization of this technique for real-world diagnostic devices. Unlike available chips and capillary-based microfluidic devices, we report milliliter-scale platform able to accommodate 1ml of a sample for acoustophoresis based processing on a market available glass capillary. Although it is presented as a generic platform but as a demonstration we have shown that polystyrene suspending medium sample can be processed with trapping efficiency of 87% and the up-concentration factor of 10 times in a flow through manner i.e., at 35µl/min. For stationary volume accommodation, this platform practically offers 50 times more sample handling capacity than most of the microfluidic setups. Furthermore, we have also shown that with diluted blood (0.6%) in a flow-through manner, 82% of the white blood cells (WBCs) per ml could be kept trapped. This milliliter platform could potentially be utilized for assisting in sample preparation, plasma separation as well as a flow-through immunoassay assay development for clinical diagnostic applications.

  • 29. Fornell, Anna
    et al.
    Nilsson, Johan
    Jonsson, Linus
    Periyannan Rajeswari, Prem Kumar
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Jönsson, Håkan
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Tenje, Maria
    Particle enrichment in two-phase microfluidic systems using acoustophoresis2016Ingår i: Acoustofluidics 2016, Kongens Lyngby, Denmark, September 22-23 2016, 2016Konferensbidrag (Refereegranskat)
  • 30.
    FOROUGHI ASL, HASSAN
    KTH, Skolan för datavetenskap och kommunikation (CSC).
    Inherited Risk Enrichment Analysis ofgene sets using Genome-wide AssociationStudies for Coronary Artery Disease2013Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Genome-wide association studies (GWAS) has been in the heartof medical research for the last 5 years. These studies seek forcommon variants in the genome that are linked to risk for commoncomplex diseases (CCDs). Although GWAS has defined a numberof interesting genetic loci for a range of CCDs, the current GWASanalysis has limitation such as investigating the DNA variantsone-by-one focusing on the most significant DNA variants. As aconsequence, most risk variants for CCDs are, in my belief, stillhidden in the GWAS data. Herein, I use a method of GWASanalysis that considers risk-enrichment for groups of functionallyassociated genes defined by for example gene networks, believedto play a role in CCDs.In this method, a set of expression SNP (single nucleotidepolymorphism) was selected from genes which are known to berelated to coronary artery disease (CAD) in a way that a singleeSNP was chosen for each gene. Then using the data availablefrom the International HapMap Project and a GWAS data available,it is possible to find SNPs which are in strong linkage withthe initial set, which we call it expanded set. Depending on theassociation of the initial set to the CAD, expanded set can showan enrichment score greater or smaller compared to the null distributionset of SNPs with same properties of the expanded set.In conclusions, CCDs are not a consequence of isolated geneticvariants/genes in isolated pathways but instead sets of geneticvariants/genes acting in conjunction, cause CAD. Genetic riskenrichment analysis is a fairly simple and straightforward methodto determine to what extent a group of functionally associatedgenetic variants/genes are enriched for a given CCD. In addition,this analysis can perhaps help to decipher some of the 90-85% ofrisk variation in populations that remains unaccounted.

    Ladda ner fulltext (pdf)
    fulltext
    Ladda ner (pdf)
    bilaga
  • 31.
    Fredenberg, Erik
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik.
    Cederström, Björn
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik.
    Lundqvist, Mats
    Ribbing, Carolina
    Åslund, Magnus
    Diekmann, Felix
    Charité - Univ. Hospital (Germany) .
    Nishikawa, Robert
    Univ. of Chicago (United States) .
    Danielsson, Mats
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik.
    Contrast-enhanced dual-energy subtraction imaging using electronic spectrum-splitting and multi-prism x-ray lenses2008Ingår i: Medical Imaging 2008 - Physics of Medical Imaging: PTS 1-3 / [ed] Hsieh, J; Samei, E, 2008, Vol. 6913, s. 91310-91310Konferensbidrag (Refereegranskat)
    Abstract [en]

    Dual-energy subtraction imaging (DES) is a method to improve the detectability of contrast agents over a lumpy background. Two images, acquired at x-ray energies above and below an absorption edge of the agent material, are logarithmically subtracted, resulting in suppression of the signal from the tissue background and a relative enhancement of the signal from the agent. Although promising, DES is still not widely used in clinical practice. One reason may be the need for two distinctly separated x-ray spectra that are still close to the absorption edge, realized through dual exposures which may introduce motion unsharpness. In this study, electronic spectrum-splitting with a silicon-strip detector is theoretically and experimentally investigated for a mammography model with iodinated contrast agent. Comparisons are made to absorption imaging and a near-ideal detector using a signal-to-noise ratio that includes both statistical and structural noise. Similar to previous studies, heavy absorption filtration was needed to narrow the spectra at the expense of a large reduction in x-ray flux. Therefore, potential improvements using a chromatic multi-prism x-ray lens (MPL) for filtering were evaluated theoretically. The MPL offers a narrow tunable spectrum, and we show that the image quality can be improved compared to conventional filtering methods.

    Ladda ner fulltext (pdf)
    fulltext
  • 32.
    Fredenberg, Erik
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik. KTH, Skolan för bioteknologi (BIO), Centra, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Cederström, Björn
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik. KTH, Skolan för bioteknologi (BIO), Centra, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Åslund, Magnus
    Nillius, Peter
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik. KTH, Skolan för bioteknologi (BIO), Centra, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Lundqvist, Mats
    Danielsson, Mats
    KTH, Skolan för teknikvetenskap (SCI), Fysik, Medicinsk bildfysik. KTH, Skolan för bioteknologi (BIO), Centra, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Imaging with multi-prism x-ray lenses2008Ingår i: Medical Imaging 2008 - Physics of Medical Imaging: PTS 1-3 / [ed] Hsieh, J; Samei, E, 2008, Vol. 6913, s. 91308-91308Konferensbidrag (Refereegranskat)
    Abstract [en]

    The multi-prism lens (MPL) is a refractive x-ray lens consisting of two rows of prisms facing each other at an angle. Rays entering the lens at the periphery will encounter a larger number of prisms than will central ones, hence experiencing a greater refraction. The focusing effect of the MPL can be used to gather radiation from a large aperture onto a smaller detector, and accordingly to make better use of the available x-ray flux in medical x-ray imaging. Potential advantages of a better photon economy include shorter acquisition times, a reduced tube loading, or an improved resolution. Since the focusing effect is one-dimensional it matches the design of scanning systems. In this study we present the first images acquired with an MPL instead of the pre-breast slit collimator in a scanning mammography system. According to the measurements, the MPL is able to increase the flux 32% at equal resolution compared to the slit collimator, or to improve the resolution 2.4 mm(-1) at equal flux. If used with a custom-made absorption filter in a clinical set-up, the gain of flux of the MPL is expected to be at least 45%, and the corresponding improvement in resolution to be 3 mm(-1).

  • 33.
    Gasser, T. Christian
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Hållfasthetslära (Inst.), Biomekanik. KTH, Skolan för teknikvetenskap (SCI), Teknisk mekanik, Farkostteknik och Solidmekanik, Hållfasthetslära.
    Auer, M.
    Biasetti, Jacopo
    KTH, Skolan för teknikvetenskap (SCI), Hållfasthetslära (Inst.), Biomekanik.
    Structural and Hemodynamical analysis of Aortic Aneurysms from Computerized Tomography Angiography data2010Ingår i: World congress on medical physics and biomedical engineering, vol 25, pt 4: Image processing, biosignal processing, modelling and simulation, biomechanics, 2010, s. 1584-1587Konferensbidrag (Refereegranskat)
    Abstract [en]

    Evaluating rupture risk of Abdominal Aortic Aneurysms is critically important in reducing related mortality without unnecessarily increasing the rate of elective repair. According to the current clinical practice aneurysm rupture risk is (mainly) estimated from its maximum diameter and/or expansion rate; an approach motivated from statistics but known to fail often in individuals. In particular, the role of the Intraluminal Thrombus is unclear and further research is required to investigate and understand its multiple impacts on aneurysm disease. Biomechanical simulations might be helpful to explore this question, however, model development is time consuming and operator-variability limits their reliability. In this study we propose an automatic procedure to develop hemodynamic and structural models of healthy and diseased abdominal aortas, where Deformable Models segment Computerized Tomography Angiography data. In total 29 numerical models of the health and diseased abdominal aorta have been developed to investigate aneurysm's rupture risk and hemodynamic consequences of aneurismal dilations. The derived results underline the suitability of biomechanical simulations to enrich diagnostic information and to uncover mechanisms of aneurysm pathology.

  • 34. Gharehbaghi, A.
    et al.
    Ask, P.
    Nylander, E.
    Janerot-Sjoberg, Birgitta
    KTH, Skolan för teknik och hälsa (STH). Karolinska Institutet.
    Ekman, I.
    Lindén, M.
    Babic, A.
    A hybrid model for diagnosing sever aortic stenosis in asymptomatic patients using phonocardiogram2015Ingår i: IFMBE Proceedings, Springer, 2015, s. 1006-1009Konferensbidrag (Refereegranskat)
    Abstract [en]

    This study presents a screening algorithm for severe aortic stenosis (AS), based on a processing method for phonocardiographic (PCG) signal. The processing method employs a hybrid model, constituted of a hidden Markov model and support vector machine. The method benefits from a preprocessing phase for an enhanced learning. The performance of the method is statistically evaluated using PCG signals recorded from 50 individuals who were referred to the echocardiography lab at Linköping University hospital. All the individuals were diagnosed as having a degree of AS, from mild to severe, according to the echocardiographic measurements. The patient group consists of 26 individuals with severe AS, and the rest of the 24 patients comprise the control group. Performance of the method is statistically evaluated using repeated random sub sampling. Results showed a 95% confidence interval of (80.5%-82.8%) /(77.8%- 80.8%) for the accuracy/sensitivity, exhibiting an acceptable performance to be used as decision support system in the primary healthcare center.

  • 35.
    Gizaw, Solomon T.
    et al.
    Indiana Univ, Dept Chem, Bloomington, IN 47405 USA.;Addis Ababa Univ, Coll Hlth Sci, Sch Med, Dept Biochem, Addis Ababa 9086, Ethiopia..
    Gaunitz, Stefan
    Indiana Univ, Dept Chem, Bloomington, IN 47405 USA.;KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Alballova Univ Ctr, Div Glycosci,Dept Chem, SE-10691 Stockholm, Sweden..
    Novotny, Milos V.
    Indiana Univ, Dept Chem, Bloomington, IN 47405 USA..
    Highly Sensitive O-Glycan Profiling for Human Serum Proteins Reveals Gender-Dependent Changes in Colorectal Cancer Patients2019Ingår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 91, nr 9, s. 6180-6189Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A newly developed microscale protocol for profiling serum O-glycans has been validated here with multiple serum samples obtained from different cohorts of colorectal cancer patients. The simultaneous cleavage and permethylation steps in this procedure preserve the integrity of released minor O-glycans, so that 39 O-linked oligosaccharides could be reliably recorded in a profile. This is far more detected components than shown in any previous studies. The analytical results were further subjected to a battery of statistical tests. Our O-glycan compositions compare favorably with the previous results obtained with solid tumors and cancer cell lines, suggesting that smaller circulatory mucins protruding into the blood circulation may be one source of O-glycans that we observe in the serum samples. While the control vs cancer statistical comparisons generally agree with the expected glycosylation trends, the comparisons of male vs female subjects have led to some surprising results for which we do not have a ready explanation due to lack of any literature describing hormonal control of O-glycosylation. Our results thus underscore the necessity of applying new analytical technologies to clinically interesting sample sets.

  • 36.
    Govind, Satish C.
    KTH, Skolan för teknik och hälsa (STH), Medicinsk teknik.
    Myocardial Effects of Type 2 Diabetes, Co-morbidities, and Changing Loading Conditions: a Clinical Study by Tissue Velocity Echocardiography2007Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Ever since the validation of the tissue velocity echocardiography (TVE) technique more than a decade ago the modality has been used rather successfully in various clinical situations, at rest as well as during stress echocardiography. Hitherto, dobutamine stress echocardiography has been the hallmark of all forms of stress procedures, now with TVE, quantification of the longitudinal motions of the left ventricle shows far superiority, with improved sensitivity and specificity in the functional diagnosis of coronary artery disease. Morever there has been continued interest in this technique for even assessing subclinical myocardial systolic and diastolic function in clinical scenarios like diabetes, hypertension and chronic kidney disease.

    The aim of the present study was to evaluate left ventricular myocardial functions by applying TVE in human subjects having type 2 diabetes with or without co-morbidities and during changing loading conditions. The effects of changing loading conditions were analyzed during hemodialysis and following oral administration of an AT1 receptor blocker. The studied subjects included individuals with diabetes as well as those with associated hypertension, coronary artery disease, microalbuminuria and end-stage renal disease. All patients with type 2 diabetes and co-morbidities underwent TVE enhanced dobutamine stress echocardiography while load dependant left ventricular functions were analyzed at rest. There were 270 subjects in the study of type 2 diabetes and associated cardiovascular diseases and 101 subjects in the study of changing loading conditions.

    Patients with type 2 diabetes revealed subclinical left ventricular dysfunction characterized by reduced functional reserve. This influence becomes quantitatively more pronounced in the presence of coexistent coronary artery disease and hypertension. The coexistence of type 2 diabetes and hypertension appears to have additive negative effect on both systolic and diastolic left ventricular function, even in the absence of coronary artery disease. The presence of microalbuminuria in type 2 diabetes patients does not worsen diminished myocardial functional reserve. A single session of hemodialysis improves left ventricular function in patients with end-stage renal disease only in the absence of type 2 diabetes and co-morbidities, while a single dose of an AT1 receptor blocker valsartan results in reduction of afterload and, subsequently, in improvement of left ventricular function. TVE appears to be a sensitive tool for objective assessment of left ventricular function and can be successfully applied for the clinical evaluation of the effect of type 2 diabetes and co-morbidities on myocardial performance.

    Ladda ner fulltext (pdf)
    FULLTEXT01
  • 37.
    Gyllensten, Illapha Cuba
    et al.
    KTH, Skolan för datavetenskap och kommunikation (CSC).
    Bonomi, Alberto G.
    Identifying Types of Physical Activity With a Single Accelerometer: Evaluating Laboratory-trained Algorithms in Daily Life2011Ingår i: IEEE Transactions on Biomedical Engineering, ISSN 0018-9294, E-ISSN 1558-2531, Vol. 58, nr 9, s. 2656-2663Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Accurate identification of physical activity types has been achieved in laboratory conditions using single-site accelerometers and classification algorithms. This methodology is then applied to free-living subjects to determine activity behavior. This study is aimed at analyzing the reproducibility of the accuracy of laboratory-trained classification algorithms in free-living subjects during daily life. A support vector machine (SVM), a feed-forward neural network (NN), and a decision tree (DT) were trained with data collected by a waist-mounted accelerometer during a laboratory trial. The reproducibility of the classification performance was tested on data collected in daily life using a multiple-site accelerometer augmented with an activity diary for 20 healthy subjects (age: 30 +/- 9; BMI: 23.0 +/- 2.6 kg/m(2)). Leave-one-subject-out cross validation of the training data showed accuracies of 95.1 +/- 4.3%, 91.4 +/- 6.7%, and 92.2 +/- 6.6% for the SVM, NN, and DT, respectively. All algorithms showed a significantly decreased accuracy in daily life as compared to the reference truth represented by the IDEEA and diary classifications (75.6 +/- 10.4%, 74.8 +/- 9.7%, and 72.2 +/- 10.3%; p<0.05). In conclusion, cross validation of training data overestimates the accuracy of the classification algorithms in daily life.

  • 38.
    Hansson, Sven Ove
    KTH, Tidigare Institutioner (före 2005), Teknik- och vetenskapshistoria.
    The ethics of biobanks2004Ingår i: Cambridge Quarterly of Healthcare Ethics, ISSN 0963-1801, E-ISSN 1469-2147, Vol. 13, nr 4, s. 319-326Artikel i tidskrift (Refereegranskat)
  • 39. Hartmann, R. W.
    et al.
    Pijnappel, M.
    Nilvebrant, Johan
    KTH, Skolan för bioteknologi (BIO), Centra, Albanova VinnExcellence Center for Protein Technology, ProNova. KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Proteinteknologi.
    Helgudottir, H. R.
    Asbjarnarson, A.
    Traustadottir, G. A.
    Gudjonsson, T.
    Nygren, Per-Åke
    KTH, Skolan för bioteknologi (BIO), Centra, Albanova VinnExcellence Center for Protein Technology, ProNova. KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap.
    Lehmann, F.
    Odell, L. R.
    The Wittig bioconjugation of maleimide derived, water soluble phosphonium ylides to aldehyde-tagged proteins2021Ingår i: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 19, nr 47, s. 10417-10423Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Herein we disclose the transformation of maleimides into water-soluble tris(2-carboxyethyl)phosphonium ylides and their subsequent application in the bioconjugation of protein- and peptide-linked aldehydes. The new entry into Wittig bioconjugate chemistry proceeds under mild conditions and relies on highly water soluble reagents, which are likely already part of most biochemists' inventory. 

  • 40.
    Hauser, Janosch
    et al.
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Dale, Matilda
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Affinitets-proteomik.
    Beck, Olof
    Karolinska Institutet, Clinical Neuroscience, 17177 Stockholm, Sweden..
    Schwenk, Jochen M.
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Affinitets-proteomik.
    Stemme, Göran
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Fredolini, Claudia
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Affinitets-proteomik.
    Roxhed, Niclas
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    A Microfluidic Device for Patient-Centric Multiplexed Assays with Readout in Centralized Laboratories2022Manuskript (preprint) (Övrigt vetenskapligt)
  • 41.
    Hauser, Janosch
    et al.
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Lenk, Gabriel
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Ullah, Shahid
    Karolinska Univ Hosp, Clin Pharmacol, S-11486 Stockholm, Sweden..
    Beck, Olof
    Karolinska Univ Hosp, Clin Pharmacol, S-11486 Stockholm, Sweden..
    Stemme, Göran
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Roxhed, Niclas
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    An Autonomous Microfluidic Device for Generating Volume-Defined Dried Plasma Spots2019Ingår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 91, nr 11, s. 7125-7130Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Obtaining plasma from a blood sample and preparing it for subsequent analysis is currently a laborious process involving experienced health-care professionals and centrifugation. We circumvent this by utilizing capillary forces and microfluidic engineering to develop an autonomous plasma sampling device that filters and stores an exact amount of plasma as a dried plasma spot (DPS) from a whole blood sample in less than 6 min. We tested 24 prototype devices with whole blood from 10 volunteers, various input volumes (40-80 mu L), and different hematocrit levels (39-45%). The resulting mean plasma volume, assessed gravimetrically, was 11.6 mu L with a relative standard deviation similar to manual pipetting (3.0% vs 1.4%). LC-MS/MS analysis of caffeine concentrations in the generated DPS (12 duplicates) showed a strong correlation (R-2 = 0.99) to, but no equivalence with, concentrations prepared from corresponding plasma obtained by centrifugation. The presented autonomous DPS device may enable patient-centric plasma sampling through minimally invasive finger-pricking and allow generatation of volume-defined DPS for quantitative blood analysis.

  • 42. Hernandez, Fidel
    et al.
    Wu, Lyndia C.
    Yip, Michael C.
    Laksari, Kaveh
    Hoffman, Andrew R.
    Lopez, Jaime R.
    Grant, Gerald A.
    Kleiven, Svein
    KTH, Skolan för teknik och hälsa (STH), Medicinsk teknik, Neuronik.
    Camarillo, David B.
    Six Degree-of-Freedom Measurements of Human Mild Traumatic Brain Injury2015Ingår i: Annals of Biomedical Engineering, ISSN 0090-6964, E-ISSN 1573-9686, Vol. 43, nr 8, s. 1918-1934Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This preliminary study investigated whether direct measurement of head rotation improves prediction of mild traumatic brain injury (mTBI). Although many studies have implicated rotation as a primary cause of mTBI, regulatory safety standards use 3 degree-of-freedom (3DOF) translation-only kinematic criteria to predict injury. Direct 6DOF measurements of human head rotation (3DOF) and translation (3DOF) have not been previously available to examine whether additional DOFs improve injury prediction. We measured head impacts in American football, boxing, and mixed martial arts using 6DOF instrumented mouthguards, and predicted clinician-diagnosed injury using 12 existing kinematic criteria and 6 existing brain finite element (FE) criteria. Among 513 measured impacts were the first two 6DOF measurements of clinically diagnosed mTBI. For this dataset, 6DOF criteria were the most predictive of injury, more than 3DOF translation-only and 3DOF rotation-only criteria. Peak principal strain in the corpus callosum, a 6DOF FE criteria, was the strongest predictor, followed by two criteria that included rotation measurements, peak rotational acceleration magnitude and Head Impact Power (HIP). These results suggest head rotation measurements may improve injury prediction. However, more 6DOF data is needed to confirm this evaluation of existing injury criteria, and to develop new criteria that considers directional sensitivity to injury.

  • 43.
    Hertz, Hans M.
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
    Hemberg, O.
    Otendal, M.
    Tuohimaa, T.
    Hansson, B. A. M.
    Electron-Impact Liquid-Metal-Jet Hard x-Ray Sources2014Ingår i: Comprehensive Biomedical Physics, Elsevier, 2014, Vol. 8, s. 91-109Kapitel i bok, del av antologi (Övrigt vetenskapligt)
    Abstract [en]

    The power and brightness of electron-impact microfocus x-ray sources have long been limited by thermal damage in the target. This is a major constraint for a wide range of biomedical applications, from imaging to diffraction. Here, we describe the development of an x-ray microfocus source based on a new target concept, the liquid-metal jet (LMJ). The regenerative nature of this target allows for significantly higher e-beam power density than on conventional targets, resulting in this source showing promise for >. 100. × higher brightness than state-of-the-art sources. We first discuss the basic physics of the two important subsystems of the source, LMJ in vacuum and focused electron-beam systems, and then describe the properties of several versions of the source, from early prototypes to the first LMJ sources now reaching the market. Finally, we review some early applications of the source for biomedical imaging and diffraction.

  • 44.
    Hjelm, Barbara
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik.
    Brennan, Donal J
    Zendehrokh, Nooreldin
    Eberhard, Jakob
    Nodin, Björn
    Gaber, Alexander
    Pontén, Fredrik
    Johannesson, Henrik
    Smaragdi, Kristina
    Frantz, Christian
    Hober, Sophia
    KTH, Skolan för bioteknologi (BIO), Proteomik.
    Johnson, Louis B
    Påhlman, Sven
    Jirström, Karin
    Uhlén, Mathias
    KTH, Skolan för bioteknologi (BIO), Proteomik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    High nuclear RBM3 expression is associated with an improved prognosis in colorectal cancer2011Ingår i: Proteomics. Clinical applications, ISSN 1862-8354, Vol. 5, nr 11-12, s. 624-35Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: In this study, we investigated the prognostic impact of human RBM3 expression in colorectal cancer using tissue microarray-based immunohistochemical analysis. Experimental design: One polyclonal antibody and four monoclonal anti-RBM3 antibodies were generated and epitope mapped using two different methods. Bacterial display revealed five distinct epitopes for the polyclonal antibody, while the four mouse monoclonal antibodies were found to bind to three of the five epitopes. A peptide suspension bead array assay confirmed the five epitopes of the polyclonal antibody, while only one of the monoclonal antibodies could be mapped using this approach. Antibody specificity was confirmed by Western blotting and immunohistochemistry, including siRNA-mediated knock-down. Two of the antibodies (polyclonal and monoclonal) were subsequently used to analyze RBM3 expression in tumor samples from two independent colorectal cancer cohorts, one consecutive cohort (n=270) and one prospectively collected cohort of patients with cancer of the sigmoid colon (n=305). RBM3-expression was detected, with high correlation between both antibodies (R=0.81, p<0.001). Results: In both cohorts, tumors with high nuclear RBM3 staining had significantly prolonged the overall survival. This was also confirmed in multivariate analysis, adjusted for established prognostic factors. Conclusion and clinical relevance: These data demonstrate that high tumor-specific nuclear expression of RBM3 is an independent predictor of good prognosis in colorectal cancer.

  • 45.
    Holden, Richard J.
    KTH, Skolan för teknik och hälsa (STH), Ergonomi.
    Physicians' beliefs about using EMR and CPOE: In pursuit of a contextualized understanding of health IT use behavior2010Ingår i: International Journal of Medical Informatics, ISSN 1386-5056, E-ISSN 1872-8243, Vol. 79, nr 2, s. 71-80Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: To identify and describe physicians' beliefs about use of electronic medical records (EMR) and computerized provider order entry (CPOE) for inpatient and outpatient care, to build an understanding of what factors shape information technology (IT) use behavior in the unique context of health care delivery. Methods: Semi-structured qualitative research interviews were carried out, following the beliefs elicitation approach. Twenty physicians from two large Midwest US hospitals participated. Physicians were asked questions to elicit beliefs and experiences pertaining to their use of EMR and CPOE. Questions were based on a broad set of behavior-shaping beliefs and the methods commonly used to elicit those beliefs. Results: Qualitative analysis revealed numerous themes related to the perceived emotional and instrumental outcomes of EMR and CPOE use; perceived external and personal normative pressure to use those systems; perceived volitional control over use behavior; perceived facilitators and barriers to system use; and perceptions about the systems and how they were implemented. EMR and CPOE were commonly believed to both improve and worsen the ease and quality of personal performance, productivity and efficiency, and patient outcomes. Physicians felt encouraged by employers and others to use the systems but also had personal role-related and moral concerns about doing so. Perceived facilitators and barriers were numerous and had their sources in all aspects of the work system. Conclusion: Given the breadth and detail of elicited beliefs, numerous design and policy implications can be identified. Additionally, the findings are a first step toward developing a theory of health IT acceptance and use contextualized to the unique setting of health care.

  • 46.
    Häggmark, Anna
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Mikus, Maria
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Mohsenchian, Atefeh
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Hong, Mun-Gwan
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Forsström, Björn
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Gajewska, Beata
    Baranczyk-Kuzma, Anna
    Uhlén, Mathias
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Schwenk, Jochen M.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Kuzma-Kozakiewicz, Magdalena
    Nilsson, Peter
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Plasma profiling revelas three proteins associated to amyotrophic lateral sclerosis2014Ingår i: Annals of Clinical and Translational Neurology, ISSN 2328-9503, Vol. 1, nr 8, s. 544-553Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    OBJECTIVE: Amyotrophic lateral sclerosis (ALS) is the most common adult motor neuron disease leading to muscular paralysis and death within 3-5 years from onset. Currently, there are no reliable and sensitive markers able to substantially shorten the diagnosis delay. The objective of the study was to analyze a large number of proteins in plasma from patients with various clinical phenotypes of ALS in search for novel proteins or protein profiles that could serve as potential indicators of disease.

    METHODS: Affinity proteomics in the form of antibody suspension bead arrays were applied to profile plasma samples from 367 ALS patients and 101 controls. The plasma protein content was directly labeled and protein profiles obtained using 352 antibodies from the Human Protein Atlas targeting 278 proteins. A focused bead array was then built to further profile eight selected protein targets in all available samples.

    RESULTS: Disease-associated significant differences were observed and replicated for profiles from antibodies targeting the proteins: neurofilament medium polypeptide (NEFM), solute carrier family 25 (SLC25A20), and regulator of G-protein signaling 18 (RGS18).

    INTERPRETATION: Upon further validation in several independent cohorts with inclusion of a broad range of other neurological disorders as controls, the alterations of these three protein profiles in plasma could potentially provide new molecular markers of disease that contribute to the quest of understanding ALS pathology.

    Ladda ner fulltext (pdf)
    fulltext
  • 47.
    Häggmark, Anna
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Zandian, Arash
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Forsström, Björn
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Uhlén, Mathias
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Schwenk, Jochen M.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Nilsson, Peter
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Autoantibody targets in vaccine-associated narcolepsyManuskript (preprint) (Övrigt vetenskapligt)
  • 48.
    Iseri, Emre
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Microfluidic Compartmentalization for Smart Materials, Medical Diagnostics and Cell Therapy2022Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [sv]

    Organisering av vätskor i små fack är allmänt förekommande i nature, t.ex. i den cellulära sammansättningen av allt liv. Det här arbetet utforskar ett flertal ingenjörsmässiga tillvägagångssätt där organisering av vätska på mikroskala kan frambringa nya egenskaper hos material eller uppnå ny funktionalitet i life science eller medicin.

    Här introduceras fyra unika sätt att dela upp vätskor: 1) 3-Dimensionell självorganisation av vätskor i mikrostrukturer (FLUID3EAMS), 2) Mikrokapillära 2D-matriser på en mätsticka (Digital Dipstick), 3) en glidande mikrofluidisk platform med tvärflöde (Slip-X-Chip), och 4) uppdelning genom skärande av mjuk solid material (Solidify & Cut). De här metoderna användes i flertalet applikationsområden. 

    Inom området smarta material applicerade vi FLUID3EAMS för att syntetisera material med permeabilitet och ytenergi som kunde styras med temperatur och för att etablera, i välkontrollerade former, vävnadslika material i form av ett nätverk av 3-Dimensionella dubbellager av droppgränssnitt. 

    Inom området medicinsk diagnostic, applicerade vi Digital Dipstick för att utföra snabb, digital odling av bakteriekulturer i ett mätstickeformat och uppnådde kliniskt relevanta diagnostiska resultat från patienter med urinvägsinfektion. En vidareutveckling av detta koncept, Slip-X-Chip, möjliggör partikelkoncentration och sköljning som tillagda funktioner i glidande mikrofluidiska plattformar, vilket väsentligt utökar deras potentiella användningsområden. 

     Slutligen, inom området cellterapi, utforskade vi mikro-inkapsling av höga koncentrationer av terapeutiska celler och presenterade en ny teknik att framställa core-shell mikrokapslar genom att utnyttja de överlägsna materialegenskaperna hos silkesmembran från spindlar. 

    Ladda ner fulltext (pdf)
    kappa
  • 49.
    Iseri, Emre
    et al.
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    Valandis Miliadis, Angelos
    van der Wijngaart, Wouter
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Intelligenta system, Mikro- och nanosystemteknik.
    A Digital Dipstick for Multiplexed Bacteria Detection2021Ingår i: The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), 10-14 October 2021, Palm Springs, USA, and hybrid online, 2021, s. 805-806Konferensbidrag (Refereegranskat)
    Abstract [en]

    In this study, we demonstrate a digital bacterial detection assay in a dipstick format that can identify and quantitate Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis, i.e., three of the most common (>90%) bacteria causing urinary tract infections (UTIs). The operation involves nothing more than dipping the stick in urine for a few seconds and let the stick incubate for 10-12 h before read-out. Each of the 180 miniaturised culture wells in the stick contain chromogenic agar and change colour if they contain a CFU. The colour of the wells identifies the presence of a specific type of bacteria; the number of coloured wells indicates the concentration. This format allows detection and quantification at a potentially low cost and without the need for complicated external equipment or technical skills. 

    Ladda ner fulltext (pdf)
    fulltext
  • 50. Jiang, Mingzhe
    et al.
    Gia, Tuan Nguyen
    Anzanpour, Arman
    Rahmani, Amir
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Industriell och Medicinsk Elektronik.
    Westerlund, Tomi
    Salantera, Sanna
    Liljeberg, Pasi
    Tenhunen, Hannu
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Industriell och Medicinsk Elektronik.
    IoT-based Remote Facial Expression Monitoring System with sEMG Signal2016Ingår i: 2016 IEEE SENSORS APPLICATIONS SYMPOSIUM (SAS 2016) PROCEEDINGS, IEEE, 2016, s. 211-216Konferensbidrag (Refereegranskat)
    Abstract [en]

    Biopotentials including Electrocardiography (ECG), Electromyography (EMG) and Electroencephalography (EEG) measure the activity of heart, muscles and brain, respectively. They can be used for noninvasive diagnostic applications, assistance in rehabilitation medicine and human-computer interaction. The concept of Internet of Things (IoT) can bring added value to applications with biopotential signals in healthcare and human-computer interaction by integrating multiple technologies such as sensors, wireless communication and data science. In this work, we present a wireless biopotentials remote monitoring and processing system. A prototype with the case study of facial expression recognition using four channel facial sEMG signals is implemented. A multivariate Gaussian classifier is trained offline from one person's surface EMG (sEMG) signals with four facial expressions: neutral, smile, frown and wrinkle nose. The presented IoT application system is implemented on the basis of an eight channel biopotential measurement device, Wi-Fi module as well as signal processing and classification provided as a Cloud service. In the system, the real-time sEMG data stream is filtered, feature extracted and classified within each data segment and the processed data is visualized in a browser remotely together with the classification result.

123 1 - 50 av 106
RefereraExporteraLänk till träfflistan
Permanent länk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf