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  • 1.
    Bernhem, Kristoffer
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Zhang, Liang
    Fontana, Jacopo M.
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
    Nilsson, Linnea
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Scott, Lena
    Brismar, Hjalmar
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
    Aperia, Anita
    Mapping the apoptotic process with super resolution microscopy in kidney cells challenged with high glucose2017Ingår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 31Artikel i tidskrift (Övrigt vetenskapligt)
  • 2.
    Fontana, Jacopo M.
    et al.
    KTH, Centra, Science for Life Laboratory, SciLifeLab. Royal Inst Technol, Dept Appl Phys, Stockholm, Sweden..
    Bernhem, Kristoffer
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. Royal Inst Technol, Dept Appl Phys, Stockholm, Sweden..
    Zhang, L.
    Karolinska Inst, Dept Pediat Cell Mol Biol, Stockholm, Sweden..
    Nilsson, Linnea
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. Royal Inst Technol, Dept Appl Phys, Stockholm, Sweden..
    Blom, Hans
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. Royal Inst Technol, Dept Appl Phys, Stockholm, Sweden..
    Brismar, Hjalmar
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. Royal Inst Technol, Dept Appl Phys, Stockholm, Sweden..
    Aperia, A.
    Karolinska Inst, Dept Pediat Cell Mol Biol, Stockholm, Sweden..
    Ouabain, a Na, K-ATPase ligand, intervenes with the onset of glucose-triggered apoptosis2015Ingår i: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 26Artikel i tidskrift (Övrigt vetenskapligt)
  • 3.
    Fontana, Jacopo M.
    et al.
    KTH, Centra, Science for Life Laboratory, SciLifeLab. Royal Inst Technol, Sci Life Labs, Appl Phys, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden..
    Jess, David Unnersjö
    KTH, Centra, Science for Life Laboratory, SciLifeLab. Royal Inst Technol, Sci Life Labs, Appl Phys, Stockholm, Sweden..
    Blom, Hans
    KTH, Centra, Science for Life Laboratory, SciLifeLab. Royal Inst Technol, Sci Life Labs, Appl Phys, Stockholm, Sweden..
    Brismar, Hjalmar
    KTH, Centra, Science for Life Laboratory, SciLifeLab. Royal Inst Technol, Sci Life Labs, Appl Phys, Stockholm, Sweden.;Karolinska Inst, Womens & Childrens Hlth, Stockholm, Sweden..
    Aperia, Anita
    Karolinska Inst, Womens & Childrens Hlth, Stockholm, Sweden..
    Role of calcium signaling for GDNF secretion, ureter branching and early nephron formation2016Ingår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 30Artikel i tidskrift (Övrigt vetenskapligt)
  • 4.
    Fontana, Jacopo M.
    et al.
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
    Khodus, Georgiy R.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Unnersjö Jess, David
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Blom, Hans
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik.
    Aperia, Anita
    Karolinska Inst, Sci Life Lab, Dept Womens & Childrens Hlth, Solna, Sweden..
    Brismar, Hjalmar
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik.
    Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney2019Ingår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 33, nr 3, s. 4089-4096Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The central role of calcium signaling during development of early vertebrates is well documented, but little is known about its role in mammalian embryogenesis. We have used immunofluorescence and time-lapse calcium imaging of cultured explanted embryonic rat kidneys to study the role of calcium signaling for branching morphogenesis. In mesenchymal cells, we recorded spontaneous calcium activity that was characterized by irregular calcium transients. The calcium signals were dependent on release of calcium from intracellular stores in the endoplasmic reticulum. Down-regulation of the calcium activity, both by blocking the sarco-endoplasmic reticulum Ca2+-ATPase and by chelating cytosolic calcium, resulted in retardation of branching morphogenesis and a reduced formation of primitive nephrons but had no effect on cell proliferation. We propose that spontaneous calcium activity contributes with a stochastic factor to the self-organizing process that controls branching morphogenesis, a major determinant of the ultimate number of nephrons in the kidney.Fontana, J. M., Khodus, G. R., Unnersjo-Jess, D., Blom, H., Aperia, A., Brismar, H. Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney.

  • 5.
    Panizza, Elena
    et al.
    Karolinska Inst, Sci Life Lab, Dept Oncol Pathol, Solna, Sweden.;Cornell Univ, Ithaca, NY USA..
    Zhang, Liang
    Karolinska Inst, Dept Womens & Childrens Hlth, Solna, Sweden..
    Fontana, Jacopo M.
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
    Hamada, Kozo
    RIKEN, Brain Sci Inst, Lab Dev Neurobiol, Saitama, Japan..
    Svensson, Daniel
    Karolinska Inst, Dept Womens & Childrens Hlth, Solna, Sweden..
    Akkuratov, Evgeny E.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Scott, Lena
    Karolinska Inst, Dept Womens & Childrens Hlth, Solna, Sweden..
    Mikoshiba, Katsuhiko
    RIKEN, Brain Sci Inst, Lab Dev Neurobiol, Saitama, Japan..
    Brismar, Hjalmar
    KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. Karolinska Inst, Dept Womens & Childrens Hlth, Solna, Sweden..
    Lehtio, Janne
    Karolinska Inst, Sci Life Lab, Dept Oncol Pathol, Solna, Sweden..
    Aperia, Anita
    Karolinska Inst, Dept Womens & Childrens Hlth, Solna, Sweden..
    Ouabain-regulated phosphoproteome reveals molecular mechanisms for Na+, K+-ATPase control of cell adhesion, proliferation, and survival2019Ingår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 33, nr 9, s. 10193-10206Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The ion pump Na+, K+-ATPase (NKA) is a receptor for the cardiotonic steroid ouabain. Subsaturating concentration of ouabain triggers intracellular calcium oscillations, stimulates cell proliferation and adhesion, and protects from apoptosis. However, it is controversial whether ouabain-bound NKA is considered a signal transducer. To address this question, we performed a global analysis of protein phosphorylation in COS-7 cells, identifying 2580 regulated phosphorylation events on 1242 proteins upon 10- and 20-min treatment with ouabain. Regulated phosphorylated proteins include the inositol triphosphate receptor and stromal interaction molecule, which are essential for initiating calcium oscillations. Hierarchical clustering revealed that ouabain triggers a structured phosphorylation response that occurs in a well-defined, time-dependent manner and affects specific cellular processes, including cell proliferation and cell-cell junctions. We additionally identify regulation of the phosphorylation of several calcium and calmodulin-dependent protein kinases (CAMKs), including 2 sites of CAMK type II-gamma (CAMK2G), a protein known to regulate apoptosis. To verify the significance of this result, CAMK2G was knocked down in primary kidney cells. CAMK2G knockdown impaired ouabain-dependent protection from apoptosis upon treatment with high glucose or serum deprivation. In conclusion, we establish NKA as the coordinator of a broad, tightly regulated phosphorylation response in cells and define CAMK2G as a downstream effector of NKA.-Panizza, E., Zhang, L., Fontana, J. M., Hamada, K., Svensson, D., Akkuratov, E. E., Scott, L., Mikoshiba, K., Brismar, H., Lehtio, J., Aperia, A. Ouabain-regulated phosphoproteome reveals molecular mechanisms for Na+, K+-ATPase control of cell adhesion, proliferation, and survival.

  • 6.
    Yin, Huijuan
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Fontana, Jacopo M.
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Solandt, Johan
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab. AstraZeneca R&D, Sweden.
    Jussi, Johnny Israelsson
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Xu, Hao
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Brismar, Hjalmar
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Fu, Ying
    KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellulär biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Quantum dots modulate intracellular Ca2+ level in lung epithelial cells2017Ingår i: International Journal of Nanomedicine, ISSN 1176-9114, E-ISSN 1178-2013, Vol. 12, s. 2781-2792Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    While adverse effects of nanoparticles on lung health have previously been proposed, few studies have addressed the direct effects of nanoparticle exposure on the airway epithelium. In this work, we examine the response of the pulmonary airway to nanoparticles by measuring intracellular Ca2+ concentration ([Ca2+](i)) in the Calu-3 epithelial layer stimulated by 3-mercaptopropionic-acid (3MPA) coated CdSe-CdS/ZnS core-multishell quantum dots (QDs). Simultaneous transient transepithelial electrical resistance (TEER) decrease and global [Ca2+](i) increase in Calu-3 epithelial layer, accompanied by cell displacements, contraction, and expansion, were observed under QD deposition. This suggests that a QD-induced global [Ca2+](i) increase in the Calu-3 epithelial layer caused the transient TEER decrease. The [Ca2+](i) increase was marked and rapid in the apical region, while [Ca2+](i) decreased in the basolateral region of the epithelial layer. TEER transient response and extracellular Ca2+ entry induced by QD deposition were completely inhibited in cells treated with stretched-activated (SA) inhibitor GdCl3 and store-operated calcium entry (SOCE) inhibitor BTP2 and in cells immersed in Ca2+-free medium. The voltage-gated calcium channel (VGCC) inhibitor nifedipine decreased, stabilized, and suppressed the TEER response, but did not affect the [Ca2+](i) increase, due to QD deposition. This demonstrates that the Ca2+ influx activated by QDs' mechanical stretch occurs through activation of both SA and SOCE channels. QD-induced [Ca2+](i) increase occurred in the Calu-3 epithelial layer after culturing for 15 days, while significant TEER drop only occurred after 23 days. This work provides a new perspective from which to study direct interactions between airway epithelium and nanoparticles and may help to reveal the pathologies of pulmonary disease.

1 - 6 av 6
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