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  • 4101.
    Allvar, Marie
    et al.
    KTH, School of Industrial Engineering and Management (ITM), Machine Design (Dept.).
    Engel, Katrin
    KTH, School of Industrial Engineering and Management (ITM), Machine Design (Dept.).
    Öhrvall Karlsson, Vincent
    KTH, School of Industrial Engineering and Management (ITM), Machine Design (Dept.).
    Planetväxelinnovation: En studie i hur omkonstruktion av planetväxlar medför effektivare tillverkning2010Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The purpose of this study is to investigate the possibility of making the manufacturing process for outer rings of planetary gears more efficient by redesign, where the use of loose cogs was the idea of the redesign. One idea was to use the cylinders of roller bearings placed in a ring with holes. This is today used in industrial robots. Another concept was to use loose cogs with the profile-shape of involutes, where the main idea was to maintain the shape of the sun gear and the planet gear. By modeling a “reference” planetary gear the necessary data for calculations was obtained. The concepts were analyzed in terms of stress and pressure with FEM and analytical methods. The analysis of the results confirmed that the concepts met the requirements for the used load and dimensions. However, the concepts require further work to ensure the size range within which loose cogs are applicable, and also to determine the exact geometry of the profile of the cog.

  • 4102.
    Allvin, Fanny
    et al.
    KTH, School of Architecture and the Built Environment (ABE), Real Estate and Construction Management.
    Sedig, Johanna
    KTH, School of Architecture and the Built Environment (ABE), Real Estate and Construction Management.
    How should the responsibility for interior maintenance be regulated? – A study of seven museums’ leases2012Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    All premises are covered by a lease which governs the rights and obligations between landlord and tenant. Contracts can be designed in different ways, but to develop a contract that takes all possible outcomes into account is basically impossible. One can still in various ways make contracts as clear as possible. One area which often causes problem of boundaries is maintenance of interior surface layers, and this is the problem that this thesis focuses on. We have studied this issue at the National Property Board (SFV) by looking at seven museums. As a landlord, SFV is strongly tied to many of their tenants because of the special facilities they manage. Because many of the tenants of SFV are strongly tied to the premises that the business is conducted in, the ability to terminate the contract is limited for both parties. It makes the relationship with the tenants extremely important since it is long-term. Even tenants cherish the relationship with SFV and short-term conflicts are rare. There is simply a lot of value in avoiding conflicts and disputes are often solved by compromises. To regulate the responsibilities and provide clarity between SFV and their tenants SFV uses detailed demarcation lists. It has been shown that these lists are rarely used because of its length and incalculability. Our judgment is that because both parties care so much about having a good relationship, they will perform their obligations, which means that such detailed demarcation lists are not necessary. Our recommendation is instead a shorter, more general list, which we believe would simplify the situation, both for SFV and their tenants.

  • 4103.
    Allvin, Fanny
    et al.
    KTH, School of Architecture and the Built Environment (ABE), Real Estate and Construction Management.
    Sedig, Johanna
    KTH, School of Architecture and the Built Environment (ABE), Real Estate and Construction Management.
    Underhåll av småhus utlagt på totalentreprenad: Finns det en efterfrågan?2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    Vi har undersökt om det finns en marknad för ett underhållsavtal för småhusägare. Vår idé är att villaägare ska kunna teckna ett avtal med ett företag som i sin tur förhandlar med ett eller flera underhållsföretag. Huset besiktigas sedan och det görs upp en underhållsplan, med de åtgärder som villaägaren önskar ska ingå. Villaägaren kommer sedan att betala in en viss summa pengar enligt avtal varje månad till företaget, som ser till att underhållet sköts. Månadskostnaden är beroende av både vilka underhållsåtgärder som ingår och husets standard vid tecknandet av avtalet.

    För att undersöka om det finns en marknad har vi intervjuat fem villaägare och två underhållsföretag. Vi har även talat med Folke Björk, professor i byggvetenskap, angående hans syn på vår idé. Alla tre parter var generellt positiva till idén. Den främsta målgruppen för ett underhållsavtal bedömer vi är barnfamiljer och välbärgade pensionärer. Både företagen och privatpersonerna ansåg att det utvändiga underhållet skulle passa bäst för ett underhållsavtal, då det invändiga underhållet inte bara utförs för att det måste utan även för att man vill ha lite omväxling och fräscha upp huset. Det invändiga underhållet går dessutom i stor utsträckning att göra själv.

    Det vi har kommit fram till är att det finns en marknad för ett underhållsavtal men att det finns många aspekter att se över innan ett underhållsavtal skulle kunna tecknas i praktiken.

  • 4104. Allvin, M.
    et al.
    Karrbom Gustavsson, Tina
    KTH, School of Industrial Engineering and Management (ITM), Industrial Economics and Management (Dept.), Industrial Economics and Management (Div.) (closed (20130101).
    Zika-Viktorsson, Annika
    KTH, School of Industrial Engineering and Management (ITM), Machine Design (Dept.), Integrated Product Development.
    Packendorff, Johann
    KTH, School of Industrial Engineering and Management (ITM), Industrial Economics and Management (Dept.).
    Project Overload: A study on work situation in complex industrial organizations2007Conference paper (Refereed)
  • 4105. Allwood, Jens
    et al.
    Cerrato, Loredana
    KTH, School of Computer Science and Communication (CSC), Speech, Music and Hearing, TMH. KTH, School of Computer Science and Communication (CSC), Centres, Centre for Speech Technology, CTT.
    Jokinen, Kristiina
    Navarretta, Costanza
    Paggio, Patrizia
    The MUMIN coding scheme for the annotation of feedback, turn management and sequencing phenomena2007In: Language resources and evaluation, ISSN 1574-020X, E-ISSN 1574-0218, Vol. 41, no 3-4, p. 273-287Article in journal (Refereed)
    Abstract [en]

    This paper deals with a multimodal annotation scheme dedicated to the study of gestures in interpersonal communication, with particular regard to the role played by multimodal expressions for feedback, turn management and sequencing. The scheme has been developed under the framework of the MUMIN network and tested on the analysis of multimodal behaviour in short video clips in Swedish, Finnish and Danish. The preliminary results obtained in these studies show that the reliability of the categories defined in the scheme is acceptable, and that the scheme as a whole constitutes a versatile analysis tool for the study of multimodal communication behaviour.

  • 4106. Ally, M.
    et al.
    Cleveland- Innes, Martha
    KTH, School of Computer Science and Communication (CSC), Media Technology and Graphic Arts, Media (closed 20111231).
    Incorporating learning objects in an online distance education course: A firsthand experience2004Conference paper (Refereed)
  • 4107. Ally, M
    et al.
    Cleveland-Innes, Marta
    Learners and learning objects: Developing learning objects with the learner in mind2005Conference paper (Refereed)
  • 4108. Ally, M.
    et al.
    Cleveland-Innes, Marta
    KTH, School of Computer Science and Communication (CSC), Media Technology and Graphic Arts, Media.
    Boskic, N
    Larwill, S
    Learner use of learning objects2005Conference paper (Refereed)
    Abstract [en]

    This article reports findings from a study exploring the generativity (Gibbons, Nelson, & Richards, 2000; Parrish, 2004) and discoverability (Friesen, 2001) of learning objects in the hands of the learner. Through the convergence of two separate pilot projects—the Canadian EduSource initiative through Athabasca University, and the researchers’ ongoing study of affective learning in online learning environments (Cleveland-Innes & Ally, 2004)—learner perspectives of learning object use and value was evaluated. Participants in the study of affective outcomes in the workplace worked independently with learning objects and outlined the interaction with learning object repositories and individual learning objects. Analysis of learners’ activity and response indicates that selection of learning object repositories and objects is based on personal needs and expectations for satisfying desired learning outcomes. Data analysis found pedagogical and contextual implications of learning object technology from the point of view of the learner. Results suggest that there is opportunity to combine learning object technology with consideration for learner engagement in designs that support lifelong learning principles and focus on learner development rather than the content or the technology. Cet article nous fait part des résultats d’une ́étude explorant la générativité (Gibbons, Nelson, & Richards, 2000; Parrish, 2004) et la facilité à trouver (Friesen, 2001) des objets d’apprentissage entre les mains des apprenants. Grace à la convergence de deux projets pilotes distincts—l’initiative EduSource de l’Université Athabasca, et l’étude en cours des auteurs de l’apprentissage affectif dans des environnements d’apprentissage en ligne (Cleveland-Innes & Ally, 2004)—la perspective des apprenants à propos de l’utilisation et de la valeur des objets d’apprentissage a été évalué. Les participants à l’étude sur les conséquences affectives en milieu de travail ont utilisé des objets d’apprentissage de manière indépendante et ont souligné l’interaction entre les dépôts d’objets et les objets d’apprentissage individuels. L’analyse de l’activité et de la réponse des apprenants indique que le choix des dépôts d’objets d’apprentissage et des objets eux-memes est basé sur les besoins personnels et les attentes de l’apprenant sur les capacités des objets à satisfaire les besoins d’apprentissage. L’analyse des données a permis d’identifier des conséquences pé́dagogiques et contextuelles de la technologie derrière les objets d’apprentissage, du point de vue de l’apprenant. Les résultats suggèrent qu’il y a une opportunité de combiner la technologie soutenant les objets d’apprentissage et une péoccupation pour l’engagement de l’apprenant grâce à des designs qui soutiennent les principes de l’apprentissage à vie et se centrent sur le développement de l’apprenant plutôt que sur le contenu de la technologie.

  • 4109. Ally, M
    et al.
    Cleveland-Innes, Marta
    Kenny, R
    Koole, M
    Park, C
    Developing a community of inquiry in a mobile learning context2009Conference paper (Refereed)
  • 4110. Ally, Mohamed
    et al.
    Cleveland-Innes, Marta
    KTH, School of Computer Science and Communication (CSC), Media Technology and Graphic Arts, Media (closed 20111231).
    Boskic, Natasha
    Larwill, Sandra
    Learner use of learning objects2006In: Journal of Distance Education, ISSN 1916-6818, Vol. 21, no 2, p. 44-57Article in journal (Refereed)
    Abstract [en]

    This article reports findings from a study exploring the generativity (Gibbons, Nelson, & Richards, 2000; Parrish, 2004) and discoverability (Friesen, 2001) of learning objects in the hands of the learner. Through the convergence of two separate pilot projects—the Canadian EduSource initiative through Athabasca University, and the researchers’ ongoing study of affective learning in online learning environments (Cleveland-Innes & Ally, 2004)—learner perspectives of learning object use and value was evaluated. Participants in the study of affective outcomes in the workplace worked independently with learning objects and outlined the interaction with learning object repositories and individual learning objects. Analysis of learners’ activity and response indicates that selection of learning object repositories and objects is based on personal needs and expectations for satisfying desired learning outcomes. Data analysis found pedagogical and contextual implications of learning object technology from the point of view of the learner. Results suggest that there is opportunity to combine learning object technology with consideration for learner engagement in designs that support lifelong learning principles and focus on learner development rather than the content or the technology.

  • 4111.
    Allégret-Bourdon, Davy
    et al.
    KTH, School of Industrial Engineering and Management (ITM), Energy Technology, Heat and Power Technology.
    Fransson, Torsten H.
    KTH, School of Industrial Engineering and Management (ITM), Energy Technology, Heat and Power Technology.
    Study of shock movement and unsteady pressure on 2D generic model2006In: Unsteady Aerodynamics, Aeroacoustics and Aeroelasticity of Turbomachines / [ed] Hall, KC; Kielb, RE; Thomas, JP, 2006, p. 409-421Conference paper (Refereed)
    Abstract [en]

    A flexible generic model has been developed at the Chair of Heat and Power Technology in order to perform fatter experiments in a more fundamental fashion. It is made of engineered flexible material and oscillate in a controlled way at non-uniform amplitude and variable frequencies. Time-resolved measurements of the unsteady surface pressures, the instantaneous model geometry as well as unsteady Schlieren visualizations are performed in order to study the shock wave motion and the aerodynamic load acting over this flexible generic bump. The model oscillates at reduced frequencies from 0.015 to 0.294 at transonic flow condition. The mode shapes of such a flexible bump strongly depends on the excitation frequency of the generic model. Schlieren pictures are obtained for an operating point characterized by an inlet Mach number of 0.63. Moreover, the presented results demonstrate that the phase of shock wave movement towards bump local motion shows a decreasing trend for the third bending mode shapes at reduced frequency higher than k=0.074. At the pressure taps located after the shock wave formation, the phase of pressure fluctuations towards bump local motion presents the same decreasing trend.

  • 4112.
    Alm, Erik
    KTH, School of Electrical Engineering and Computer Science (EECS).
    Area and Power Efficiency of Multiplier-Free Finite Impulse Response Filters2018Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    In digital radio systems, a large number of finite impulse response filters are typically used. Due to their nature of operation, such filters require many multiplication operations, leading to great costs in terms of both chip area and power consumption. For cost reduction reasons, there is a strong business case for implementing these filters without general multipliers so as to reduce the area and power consumption of the overall system.This thesis explores a method of implementing finite impulse response halfband filters without general multipliers, by using a special filter structure and replacing multipliers with sequences of binary shifts and additions. The savings in terms of area and power consumption are estimated and compared to a conventional filter (with a common structure) implementation containing general multipliers, as well as the same conventional filter implemented without general multipliers by means of manipulating its coefficients such that they can be implemented with shifts and additions.The results show that while using the special filter structure with shifts and additions consumes less area and power than a conventional filter with general multipliers, employing simpler methods to obtain coefficients implementable with shifts and additions in a conventional filter structure produces smaller filters consuming less power. Moreover, the results of this thesis show that using methods allowing for multiplier-free filter implementations with conventional filter structures seems favorable, hence further investigation of such methods is recommended. Future studies could also focus on methods applicable to filters with support for dynamic coefficients.

  • 4113.
    Alm, Erik
    et al.
    KTH, School of Electrical Engineering (EES).
    Gren, Lilian
    KTH, School of Electrical Engineering (EES).
    Real-time Scheduling in Smart Buildings2016Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 4114. Alm, Hajer Kamal
    et al.
    Ström, Göran
    Karlström, Katarina
    KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.
    Schoelkopf, Joachim
    Gane, Patrick A. C.
    Effect of excess dispersant on surface properties and liquid interactions on calcium carbonate containing coatings2010In: Nordic Pulp & Paper Research Journal, ISSN 0283-2631, E-ISSN 2000-0669, Vol. 25, no 1, p. 82-92Article in journal (Refereed)
    Abstract [en]

    The aim of this work was to identify what effects excess amount of sodium polyacrylate, a commonly used dispersant, has on the coating properties and the interaction between ink and the paper coating in offset printing. Since polyacrylate strongly interacts with calcium ions, soluble calcium salt was added to some coating colours to illustrate the impact of charge neutralization by calcium ions. It was found that the coating structure was only slightly affected by the extra addition of polyacrylate, showing some weak flocculation, whereas the surface chemistry was strongly influenced. The coatings became more polar and interacted more strongly with water. This resulted in slower ink setting and reduced ink-paper coating adhesion, especially in the presence of applied water/dampening solution, which are identified as contributory factors in ink piling and print mottle.

  • 4115.
    Alm, Henrik
    et al.
    KTH.
    Lindgren, Eidar
    KTH, School of Architecture and the Built Environment (ABE), Real Estate and Construction Management, Real Estate Planning and Land Law.
    Paulsson, Jenny
    KTH, School of Architecture and the Built Environment (ABE), Real Estate and Construction Management, Real Estate Planning and Land Law.
    Hållbar dagvattenhantering i stadsmiljö: Ansvar och finansiering2017In: Bostad 2.0: En bostadsmarknad för alla / [ed] Maria Hullgren och Mats Wilhelmsson, Stockholm: Kungliga Tekniska högskolan, 2017, p. 71-77Chapter in book (Other academic)
  • 4116.
    Alm, Jonas
    et al.
    Chalmers.
    Lindskog, Filip
    KTH, School of Engineering Sciences (SCI), Mathematics (Dept.), Mathematical Statistics.
    Foreign-currency interest-rate swaps in asset-liability management for insurers2013In: European Actuarial Journal, ISSN 2190-9733, E-ISSN 2190-9741, Vol. 3, no 1, p. 133-158Article in journal (Refereed)
    Abstract [en]

    We consider an insurer with purely domestic business whose liabilities towards its policy holders have long durations. The relative shortage of domestic government bonds with long maturities makes the insurer’s net asset value sensitive to fluctuations in the zero rates used for liability valuation. Therefore, in order to increase the duration of the insurer’s assets, it is common practice for insurers to take a position as the fixed-rate receiver in an interest-rate swap. We assume that this is not possible in the domestic currency but in a foreign currency supporting a larger market of interest-rate swaps. Monthly data over 16 years are used as the basis for investigating the risks to the future net asset value of the insurer from using foreign-currency interest-rate swaps as a proxy for domestic ones in asset–liability management. We find that although a suitable position in swaps may reduce the standard deviation of the future net asset value it may significantly increase the exposure to tail risk that has a substantial effect on the estimation of the solvency capital requirements.

  • 4117.
    Alm, Jonathan
    KTH, School of Industrial Engineering and Management (ITM), Energy Technology, Heat and Power Technology.
    Biogasproduktion på Utö2013Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The purpose of this study was to investigate the conditions and opportunities to produce biogas at Utö, an island in the Stockholm archipelago. The intention was that the sludge from the local sewage treatment plant could be used as a resource. The Master Thesis was conducted as part of the EU-funded project Green Islands, where the Archipelago Foundation in Stockholm County is Lead Partner.

    In addition to the sludge, other possible substrates were investigated. Food waste from Utö Inn and slaughterhouse waste from a small slaughterhouse were determined best suited. An estimate of the amounts of substrate gave that a suitable size of a biogas reactor would be about 50 m3. Local uses of produced gas and the digestate were investigated. Several small scale biogas digesters were investigated. The closest examined digesters are the research reactor Renowaste at Henriksdalsberget, MR120 which is developed by Energiutvecklarna and an ordinary, small scale digester constructed by concrete segments. The microbiology of the biogas process has also been studied and potential difficulties were analyzed. Practical, economic and environmental aspects were examined.

    If CHP were to be applied, electricity and heat could be utilized at the waste water treatment plant. No suitable usage of the nutrient-rich digestate was found. The nearby organic farm could not use the digestate since human sludge cannot be spread on organic farming land.

    The report states that the construction of a biogas plant in Gruvbyn on Utö cannot be economically justified. Several practical problems were also noted, where mainly the small scale and the uneven flows of substrates are expected to contribute. The low carbon/nitrogen ratio of the substrates, about 8, could also pose difficulties in obtaining a stable process.Environmental and climatic benefits deemed relatively small, mainly due to that upgrading of biogas to vehicle fuel, thus replacing fossil fuels, cannot be seen as a realistic possibility.

    A number of possible improvements that are considered more realistically feasible are suggested, like local treatment of sludge in a bed of reed, making the heating system of the waste water plant more efficient and local composting of the inn’s food waste.

  • 4118.
    Alm, Karl-Johan
    KTH, School of Information and Communication Technology (ICT).
    Evaluation of a Prototype for Relevance Profiling2013Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Only a small portion of the amounts of information generated online are relevant to a given person.

    In this thesis, a prototype for determining a relevance value based on sets of data for some topic is evaluated to determine its viability in a future product called Votia.

    To achieve this, an evaluation model was dened based on \accuracy" and \eciency" for various machine learning algorithms applied to various types of data found in a tweet - a short user message on the Twitter platform | such as the message, relations between users and the tweeter, users' general behavior characteristics, and geographic data. A system was set up to fetch and convert Twitter data into data tting the prototype, with the hypothesis that (1) the Twitter data model could be mapped into the Votia data model, from which user behavior could be predicted at an adequate accuracy, and that (2) user behavior could be predicted to some degree from isolated sets of data.

    Data from Twitter was obtained by taking a random sample of users - the main actors - and then loading their and their friends' timelines. The data was processed, identifying interactivity between the set of users and their friends, in particular in who retweeted what. A number of machine learning algorithms, such as Nave Bayes classier, were tested on this data and evaluated according to the model.

    In the case of user relation, data was instead obtained by identifying a number of the top Twitter users, and the evaluation revolved around grouping their followers based on how similarly they behaved.

    The evaluation shows that predicting user behavior from isolated sets of data is not applicable in the given environment, and that the data set must be analyzed in a more integrated manner, e.g. by grouping similar users together. As the input data sets are arbitrary, each being analyzed in specic ways, a pipeline with processing modules that not only analyze the data sets in terms of relevance, but also perform preprocessing, is suggested. Examples of preprocessing might be ltering, adjusting data for use by subsequent modules, or at out rejecting the data prematurely.

  • 4119. Alm, L.
    et al.
    André, M.
    Vaivads, Andris
    Khotyaintsev, Y. V.
    Torbert, R. B.
    Burch, J. L.
    Ergun, R. E.
    Lindqvist, Per-Arne
    KTH, School of Electrical Engineering and Computer Science (EECS), Space and Plasma Physics.
    Russell, C. T.
    Giles, B. L.
    Mauk, B. H.
    Magnetotail Hall Physics in the Presence of Cold Ions2018In: Geophysical Research Letters, ISSN 0094-8276, E-ISSN 1944-8007, Vol. 45, no 20, p. 10,941-10,950Article in journal (Refereed)
    Abstract [en]

    We present the first in situ observation of cold ionospheric ions modifying the Hall physics of magnetotail reconnection. While in the tail lobe, Magnetospheric Multiscale mission observed cold (tens of eV) E × B drifting ions. As Magnetospheric Multiscale mission crossed the separatrix of a reconnection exhaust, both cold lobe ions and hot (keV) ions were observed. During the closest approach of the neutral sheet, the cold ions accounted for ∼30% of the total ion density. Approximately 65% of the initial cold ions remained cold enough to stay magnetized. The Hall electric field was mainly supported by the j × B term of the generalized Ohm's law, with significant contributions from the ∇·P e and v c ×B terms. The results show that cold ions can play an important role in modifying the Hall physics of magnetic reconnection even well inside the plasma sheet. This indicates that modeling magnetic reconnection may benefit from including multiscale Hall physics.

  • 4120. Alm, L.
    et al.
    Argall, M. R.
    Torbert, R. B.
    Farrugia, C. J.
    Burch, J. L.
    Ergun, R. E.
    Russell, C. T.
    Strangeway, R. J.
    Khotyaintsev, Y. V.
    Lindqvist, Per-Arne
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Marklund, Göran
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Giles, B. L.
    Shuster, J.
    EDR signatures observed by MMS in the 16 October event presented in a 2-D parametric space2017In: Journal of Geophysical Research - Space Physics, ISSN 2169-9380, E-ISSN 2169-9402, Vol. 122, no 3, p. 3262-3276Article in journal (Refereed)
    Abstract [en]

    We present a method for mapping the position of satellites relative to the X line using the measured B-L and B-N components of the magnetic field and apply it to the Magnetospheric multiscale (MMS) encounter with the electron diffusion region (EDR) which occurred on 13:07 UT on 16 October 2015. Mapping the data to our parametric space succeeds in capturing many of the signatures associated with magnetic reconnection and the electron diffusion region. This offers a method for determining where in the reconnection region the satellites were located. In addition, parametric mapping can also be used to present data from numerical simulations. This facilitates comparing data from simulations with data from in situ observations as one can avoid the complicated process using boundary motion analysis to determine the geometry of the reconnection region. In parametric space we can identify the EDR based on the collocation of several reconnection signatures, such as electron nongyrotropy, electron demagnetization, parallel electric fields, and energy dissipation. The EDR extends 2-3km in the normal direction and in excess of 20km in the tangential direction. It is clear that the EDR occurs on the magnetospheric side of the topological X line, which is expected in asymmetric reconnection. Furthermore, we can observe a north-south asymmetry, where the EDR occurs north of the peak in out-of-plane current, which may be due to the small but finite guide field.

  • 4121. Alm, L.
    et al.
    Farrugia, C. J.
    Paulson, K. W.
    Argall, M. R.
    Torbert, R. B.
    Burch, J. L.
    Ergun, R. E.
    Russell, C. T.
    Strangeway, R. J.
    Khotyaintsev, Y. V.
    Lindqvist, Per-Arne
    KTH, School of Electrical Engineering and Computer Science (EECS), Space and Plasma Physics.
    Marklund, Göran
    KTH, School of Electrical Engineering and Computer Science (EECS), Space and Plasma Physics.
    Giles, B. L.
    Differing Properties of Two Ion-Scale Magnetopause Flux Ropes2018In: Journal of Geophysical Research - Space Physics, ISSN 2169-9380, E-ISSN 2169-9402, Vol. 123, no 1, p. 114-131Article in journal (Refereed)
    Abstract [en]

    In this paper, we present results from the Magnetospheric Multiscale constellation encountering two ion-scale, magnetopause flux ropes. The two flux ropes exhibit very different properties and internal structure. In the first flux rope, there are large differences in the currents observed by different satellites, indicating variations occurring over sub-d(i) spatial scales, and time scales on the order of the ion gyroperiod. In addition, there is intense wave activity and particle energization. The interface between the two flux ropes exhibits oblique whistler wave activity. In contrast, the second flux rope is mostly quiescent, exhibiting little activity throughout the encounter. Changes in the magnetic topology and field line connectivity suggest that we are observing flux rope coalescence.

  • 4122.
    Alm, Love
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Cluster investigations of the extent and altitude distribution of the auroral density cavity2015Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    The auroral density cavity constitutes the boundary between the cold, dense ionospheric plasma and the hot, tenuous plasma sheet plasma. The auroral density cavity is characterized by low electron density and particle populations modified by parallel electric fields. Inside the cavity the electron densities can be as much as a factor 100-1000 lower than same altitude outside the cavity.The Cluster mission's wide range of instruments, long lifetime and ability to make multi-spacecraft observations has been very successful. Over its 15 year lifespan, the Cluster satellites have gathered data on auroral density cavities over a large altitude range and throughout an entire solar cycle, providing a vast data material.The extent of the density cavity and acceleration region is large compared to the typical altitude coverage of a satellite crossing the cavity. This makes it difficult to produce a comprehensive altitude/density profile from a single crossing. In order to facilitate comparisons between data from different events, we introduce a new reference frame, pseudo altitude. Pseudo altitude describes the satellites' position relative to the acceleration region, as opposed to relative to the Earth. This pseudo altitude is constructed by dividing the parallel potential drop below the satellite with the total parallel potential drop. A pseudo altitude of 0 corresponds to the bottom of the acceleration region and a pseudo altitude of 1 to the top of the acceleration region. As expected, the pseudo altitude increases with altitude. The electron density exhibits an anti-correlation with the pseudo altitude, the density becomes lower close to the upper edge of the acceleration region. The upper edge of the acceleration region is located between a geocentric altitude of 4.375 and 5.625 RE. Above the upper edge of the acceleration region, the electron density continues to decrease for the entire range of the study, 3.0-6.5 RE. This is much further than the geocentric altitude range of 2-3 RE which is suggested by previous models. We can conclude that the auroral density cavity is not confined by the auroral acceleration region, as suggested by previous models, and may extend all the way to the plasma sheet.

  • 4123.
    Alm, Love
    et al.
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Li, Bin
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Marklund, Göran
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Karlsson, Tomas
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Statistical altitude distribution of the auroral density cavity2015In: Journal of Geophysical Research - Space Physics, ISSN 2169-9380, E-ISSN 2169-9402, Vol. 120, no 2, p. 996-1006Article in journal (Refereed)
    Abstract [en]

    The statistical altitude distribution of auroral density cavities located between 3.0 and 6.5 R-E is investigated using in situ observations from flux tubes exhibiting auroral acceleration. The locations of the observations are described using a pseudo altitude derived from the distribution of the parallel potential drop above and below the satellite. The upper edge of the auroral acceleration region is observed between 4.375 and 5.625 R-E. Above 6.125 R-E, none of the events exhibit precipitating inverted V electrons, though the upward ion beam can be observed. This indicates that the satellites are located inside the same flux tube as, but above, the auroral acceleration region. The electron density decreases as we move higher into the acceleration region. The spacecraft potential continues to decrease once above the acceleration region, indicating that the density cavity extends above the acceleration region. From 3.0 to 4.375 R-E the pseudo altitude increases by 0.20 per R-E, consistent with a distributed parallel electric field. Between 4.375 and 5.625 R-E the pseudo altitude increases weakly, by 0.01 per R-E, due to an increasing number of events per altitude bin, which are occurring above the acceleration region. Above 5.625 R-E the pseudo altitude increases by 0.28 per R-E, due to a rapid increase in the number of events per altitude bin occurring above the acceleration region, indicating that the remaining parallel potential drop is concentrated in a narrow region at the upper edge of the acceleration region, rather than in a distributed parallel electric field.

  • 4124.
    Alm, Love
    et al.
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Marklund, Göran
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Karlsson, Tomas
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Electron density and parallel electric field distribution of the auroral density cavity2015In: Journal of Geophysical Research, ISSN 0148-0227, E-ISSN 2156-2202, Vol. 120, no 11, p. 9428-9441Article in journal (Refereed)
    Abstract [en]

    We present an event study in which Cluster satellites C1 and C3 encounters the flux tube of a stable auroral arc in the pre-midnight sector. C1 observes the mid cavity, while C3 enters the flux tube of the auroral arc at an altitude which is below the acceleration region, before crossing into the top half of the acceleration region. This allows us to study the boundary between the ionosphere and the density cavity, as well as large portion of the upper density cavity. The position of the two satellites, in relation to the acceleration region, is described using a pseudo altitude derived from the distribution of the parallel potential drop above and below the satellites.The electron density exhibits an anti-correlation with the pseudo altitude, indicating that the lowest electron densities are found near the top of the density cavity. Over the entire pseudo altitude range, the electron density distribution is similar to a planar sheath, formed out of a plasma sheet dominated electron distribution, in response to the parallel electric field of the acceleration region. This indicates that the parallel electric fields on the ionosphere-cavity boundary, as well as the mid cavity parallel electric fields, are part of one unified structure rather than two discrete entities.The results highlight the strong connection between the auroral density cavity and auroral acceleration as well as the necessity of studying them in a unified fashion.

  • 4125.
    Alm, Love
    et al.
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Marklund, Göran T.
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Karlsson, Tomas
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    In situ observations of density cavities extending above the auroral acceleration region2014In: Journal of Geophysical Research - Space Physics, ISSN 2169-9380, E-ISSN 2169-9402, Vol. 119, no 7, p. 5286-5294Article in journal (Refereed)
    Abstract [en]

    The uppermost part of a stable potential structure in the auroral acceleration region was studied using simultaneous observations of Cluster satellites C1 and C3. Both satellites observe a monotonically decreasing electron density as they ascend through the auroral acceleration region. As C1 exits the top of the auroral acceleration region, the electron densities continue to decrease, and the minimum electron density is reached 14 km above the upper edge of the auroral acceleration region. The electron density does not return to noncavity values until the spacecraft exits the potential structure's flux tube. The data indicate that the auroral density cavity is not confined by the potential structure and may extend above the auroral acceleration region.

  • 4126.
    Alm, Love
    et al.
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Marklund, Göran T.
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Karlsson, Tomas
    KTH, School of Electrical Engineering (EES), Space and Plasma Physics.
    Masson, A.
    Pseudo altitude: A new perspective on the auroral density cavity2013In: Journal of Geophysical Research A: Space Physics, ISSN 2169-9380, Vol. 118, no 7, p. 4341-4351Article in journal (Refereed)
    Abstract [en]

    Studying the density distribution inside the auroral density cavity is complicated by the difficulties in achieving simultaneous measurements within the same flux tube at different altitudes. Comparisons between different events are complicated by variations in both the location of the density cavity and the location of the related potential structure. Describing the spacecraft's location inside the density cavity relative to the potential structure instead of the Earth offers a more practical and consistent frame of reference, a pseudo altitude. The pseudo altitude is determined by comparing the potential drop above the spacecraft, as determined from the characteristic energy of the downward electrons, with the parallel potential drop below the spacecraft, determined from the characteristic energy of the upward ions. A pseudo altitude of 0 corresponds to the bottom of the potential structure and a pseudo altitude of 1 to the top of the structure. Seven events from 2008 were selected, each of which corresponds to a Cluster crossing of a mainly quasi-static potential structure. All of the events exhibit a consistent anticorrelation between the pseudo altitude and the electron density. No upper limit of the density cavity can be observed, while all cavities have a lower limit above a pseudo altitude of 0.33. These observations show that the auroral density cavity is predominately concentrated to the upper parts of the quasi-static potential structure.

  • 4127. Alm, M.
    et al.
    Gutierrez-Farewik, Elena M.
    Hultling, C.
    Saraste, H.
    Clinical evaluation of seating in persons with complete thoracic spinal cord injury2003In: Spinal Cord, ISSN 1362-4393, E-ISSN 1476-5624, Vol. 41, no 10, p. 563-571Article in journal (Refereed)
    Abstract [en]

    Study Design: Consecutive male patients studied with photographic measurement of a combination of clinical methods. Objectives: To describe seating in individuals with complete thoracic spinal cord injury (SCI) by using a combination of clinical methods. Setting: Spinalis SCI unit, Stockholm, Sweden. Methods: Wheelchair specifications were documented. Measurements of posture from photographs in 30 male subjects with complete thoracic SCI, sitting in a relaxed and an upright position on a standardized surface and in a wheelchair were calculated. A comparison was made between positions and seating surfaces. An examiner's classification of lower trunk position in wheelchair was compared to subjects' evaluations. SCI subjects reported sitting support, satisfaction, and wishes for improvement. Results: Most SCI subjects used similar wheelchair specifications. None of the backrests were custom designed. Relatively small differences were found between the relaxed and upright position in the wheelchair regarding measurements of posture and according to the examiner's classification of the lower trunk position. Only 13/30 SCI subjects were sitting with the lower trunk centered relative to the backrest in the upright position. The examiner's classification and the subjects' evaluation of asymmetric sitting were not always in agreement. Only 12/30 SCI subjects were satisfied with their way of sitting. Conclusion: Current wheelchair specifications and adjustments seem to inhibit a postural correction towards upright sitting and fail to provide sufficient lateral support. Findings indicate an inability for SCI subjects to vary their sitting position in a wheelchair to a large extent. Both an examiner's classification and subjects' evaluation of asymmetric sitting are necessary to obtain a sufficient knowledge base for subsequent adjustment. By using methods regarding different aspects of seating, a more comprehensive view of seating was achieved. The combination of clinical methods seems to be useful in order to describe seating in individuals with complete thoracic SCI.

  • 4128.
    Alm, Malin
    KTH, School of Biotechnology (BIO).
    Improvement study of Informed Consent Process2011Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
  • 4129.
    Alm, Malin
    KTH, School of Industrial Engineering and Management (ITM), Energy Technology, Applied Thermodynamics and Refrigeration.
    Simulering av spillvattenflödet i Solnaverkets värmepumpar2015Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Norrenergi has four heat pumps in their heating plant in Solna. These heat pumps use waste water from the Bromma sewage treatment plant as a heat source to produce district heating to distribute in Solna and Sundbyberg. This waste water is led into a duct where it is pumped through the evaporators of the heat pumps. The waste water is first pumped up into the first heating pump. The water that is not used in the first heat pump is passed by to be pumped up into the second heat pump, if it is in operation. In the winter months, the waste water flow into the duct is limited and the temperature can be lower. The consequence of this is that the waste water to heat pump 2 may have lower temperature, because it has already partially passed through the evaporator in heat pump 1.

    The aim of this study is to simulate this plant and investigate the possibility to optimize the distribution of the waste water to increase the total heating capacity of heat pump 1 and 2, and investigate if it is possible to operate the heat pumps with better economy.

    By developing heat pump specific equations based on measured data in the plant and build a model in Excel, these questions have been answered mathematically. The results of this study shows that it is not profitable to redistribute the waste water flow by reducing the flow into the evaporator of heat pump 1, to achieve a higher temperature of the water into heat pump 2. This is due to a lower flow through the evaporator in heat pump 1 affecting the overall heat transfer coefficient negatively, which in turn leads to a poorer heat transfer from the waste water to the refrigerant. It also results in a lower evaporation pressure within the evaporator of heat pump 1, which means the risk of freezing increases.

    To secure the operation of both heat pump 1 and 2, the heating capacity of heat pump 1 should be reduced to reach an incoming waste water temperature into heat pump 2 that is high enough to keep the second heat pump in operation.

  • 4130.
    ALM, RAGNAR
    et al.
    KTH, School of Industrial Engineering and Management (ITM), Industrial Economics and Management (Dept.), Industrial Marketing and Entrepreneurship.
    KYRÖNLAHTI, RUDY
    Take time to make time: What to consider when managing multi-channel sales systems with the objective to increase sales efficiency2016Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Traditional sales systems have been disrupted by technological developments. In order to  adapt, companies are changing the way they interact with their customers in business-to-business markets. In the last three decades, multi-channel strategies have spurred the proliferation of different sales channels and new ways of managing sales systems. The purpose of this research was to investigate what should be considered when managing multi-channel sales systems with the objective of increasing sales efficiency. The study has investigated current utilisation of multi-channel sales systems in the context of a business-to-business setting in industrial companies that are involved in the Swedish automotive industry. Multi-channel sales systems can be utilised to achieve many different objectives. However, this research pays specific attention on how to improve sales efficiency by utilising multi-channel sales systems in the context of a business-to-business setting. The research employed an explorative case study, where semi-structured and structured interviews were conducted at a case company and at companies that are first or second tier suppliers in the Swedish automotive industry. The qualitative data were analysed using thematic analysis. The empirical findings indicate that the most prevalent measure for increasing sales efficiency is to prioritise and allocate customers based on economic attractiveness. Furthermore, the key issues that impede sales efficiency in multi-channels sales system are misaligned sales activities, deficient prioritisation procedures, insufficient promotion of customer value and inadequate focus on customers. The findings highlight key areas to address and may provide guidelines for the design and management of multi-channel sales systems with the specific purpose of obtaining sales efficiency. The implications of this research are mainly practical and are aimed at supporting sales managers, or individuals in similar positions engaged in multi-channel sales system design and management, in obtaining sales efficiency. Managers should focus on aligning sales activities across the whole  sales system, allocate customers according to prioritisation and stay in line with market developments by understanding customer behaviours and perceptions.

  • 4131. Alm, Sven Erick
    et al.
    Janson, Svante
    Linusson, Svante
    KTH, School of Engineering Sciences (SCI), Mathematics (Dept.), Mathematics (Div.).
    Correlations for Paths in Random Orientations of G(n, p) and G(n, m)2011In: Random structures & algorithms (Print), ISSN 1042-9832, E-ISSN 1098-2418, Vol. 39, no 4, p. 486-506Article in journal (Refereed)
    Abstract [en]

    We study random graphs, both G(n, p) and G(n, m), with random orientations on the edges. For three fixed distinct vertices s, a, b we study the correlation, in the combined probability space, of the events {a -> s} and {s -> b}. For G(n, p), we prove that there is a p(c) = 1/2 such that for a fixed p < p(c) the correlation is negative for large enough n and for p > p(c) the correlation is positive for large enough n. We conjecture that for a fixed n >= 27 the correlation changes sign three times for three critical values of p. For G(n, m) it is similarly proved that, with p = m/((n)(2)), there is a critical p(c) that is the solution to a certain equation and approximately equal to 0.7993. A lemma, which computes the probability of non existence of any l directed edges in G(n, m), is thought to be of independent interest. We present exact recursions to compute P(a -> s) and P(a -> s, s -> b). We also briefly discuss the corresponding question in the quenched version of the problem.

  • 4132. Alm, Sven Erick
    et al.
    Janson, Svante
    Linusson, Svante
    KTH, School of Engineering Sciences (SCI), Mathematics (Dept.), Mathematics (Div.).
    First critical probability for a problem on random orientations in G(n,p)2014In: Electronic Journal of Probability, ISSN 1083-6489, E-ISSN 1083-6489, Vol. 19, p. 69-Article in journal (Refereed)
    Abstract [en]

    We study the random graph G (n,p) with a random orientation. For three fixed vertices s, a, b in G(n,p) we study the correlation of the events {a -> s} (there exists a directed path from a to s) and {s -> b}. We prove that asymptotically the correlation is negative for small p, p < C-1/n, where C-1 approximate to 0.3617, positive for C-1/n < p < 2/n and up to p = p(2)(n). Computer aided computations suggest that p(2)(n) = C-2/n, with C-2 approximate to 7.5. We conjecture that the correlation then stays negative for p up to the previously known zero at 1/2; for larger p it is positive.

  • 4133. Alm, Sven Erick
    et al.
    Linusson, Svante
    KTH, School of Engineering Sciences (SCI), Mathematics (Dept.), Mathematics (Div.).
    A Counter-Intuitive Correlation in a Random Tournament2011In: Combinatorics, probability & computing, ISSN 0963-5483, E-ISSN 1469-2163, Vol. 20, no 1, p. 1-9Article in journal (Refereed)
    Abstract [en]

    Consider a randomly oriented graph G = (V, E) and let a, s and b be three distinct vertices in V. We study the correlation between the events {a -> s} and {s -> b}. We show that, counter-intuitively, when G is the complete graph K-n, n >= 5, then the correlation is positive. (It is negative for n = 3 and zero for n = 4.) We briefly discuss and pose problems for the same question on other graphs.

  • 4134.
    Alm, Tove
    KTH, School of Biotechnology (BIO), Proteomics.
    Interaction engineered three-helix bundle domains for protein recovery and detection2010Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. Traditionally, monoclonal antibodies have been the natural choice for biotechnological and therapeutic applications due to their ability to bind a huge range of different molecules and their natural good affinity. However, the large size of antibodies (150 kDa) limits tissue penetration and the recombinant expression is complicated. Therefore, alternative binders with smaller sizes have been derived from antibodies and alternative scaffolds.

    In this thesis, two structurally similar domains, Zbasic and ABDz1, have been used as purification tags in different contexts. They are both three-helical bundles and derived from bacterial surface domains, but share no sequence homology. Furthermore, by redesign of the scaffold used for ABDz1, a molecule intended for drug targeting with extended in-vivo half-life has been engineered. In Papers I and II, the poly-cationic tag Zbasic is explored and evaluated. Paper I describes the successful investigation of Zbasic as a purification handle under denaturating conditions. Moreover, Zbasic is evaluated as an interaction domain in matrixassisted refolding. Two different proteins were successfully refolded using the same setup without individual optimization. In Paper II, Zbasic is further explored as a purification handle under non-native conditions in a multi-parallel setup. In total, 22 proteins with varying characteristics are successfully purified using a multi-parallel protein purification protocol and a robotic system. Without modifications, the system can purify up to 60 proteins without manual handling. Paper I and II clearly demonstrate that Zbasic can be used as an interaction domain in matrix-assisted refolding and that it offers a good alternative to the commonly used His6-tag under denaturating conditions. In paper III, the small bifunctional ABDz1 is selected from a phage display library. Endowed with two different binding interfaces, ABDz1 is capable of binding both the HSA-sepharose and the protein A-derived MabSelect SuRe-matrix. The bifunctionality of the domain is exploited in an orthogonal affinity setup. Three target proteins are successfully purified using the HSA-matrix and the MabSelect SuRe-matrix. Furthermore, the purity of the target proteins is effectively improved by combining the two chromatographic steps. Thus, paper III shows that the small ABDz1 can be used as an effective purification handle and dual affinity tag without target specific optimization. Paper IV describes the selection and affinity maturation of small bispecific drug-targeting molecules. First generation binders against tumor necrosis factor-α are selected using phage display. Thereafter on-cell surface display and flow cytometry is used to select second-generation binders. The binding to tumor necrosis factor-α is improved up to 30 times as compared to the best first generation binder, and a 6-fold improvement of the binding strength was possible with retained HSA affinity. Paper III and IV clearly demonstrate that dual interaction surfaces can successfully be grafted on a small proteinaceous domain, and that the strategy in paper IV can be used for dual selection of bifunctional binders.

  • 4135.
    Alm, Tove L.
    et al.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    Lundberg, Emma
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    Uhlén, Mathias
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    The Affinity Binder Knockdown Initiative.2016In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 27Article in journal (Refereed)
  • 4136.
    Alm, Tove L.
    et al.
    KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH Royal Inst Technol, Biotechnol, Stockholm, Sweden..
    Lundberg, Emma
    KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH Royal Inst Technol, Biotechnol, Stockholm, Sweden..
    Uhlén, Mathias
    KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH Royal Inst Technol, Biotechnol, Stockholm, Sweden..
    The Affinity Binder Knockdown Initiative2015In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 26Article in journal (Other academic)
  • 4137.
    Alm, Tove L.
    et al.
    KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH Royal Inst Technol, Biotechnol, Stockholm, Sweden..
    von Feilitzen, Kalle
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Systems Biology. KTH Royal Inst Technol, Biotechnol, Stockholm, Sweden..
    Uhlén, Mathias
    KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH Royal Inst Technol, Biotechnol, Stockholm, Sweden..
    Antibodypedia - The wiki of antibodies2015In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 26Article in journal (Other academic)
  • 4138.
    Alm, Tove L.
    et al.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    von Feilitzen, Kalle
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    Uhlén, Mathias
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
    ANTIBODYPEDIA: THE WIKI OF ANTIBODIES2016In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 27Article in journal (Refereed)
  • 4139.
    Alm, Tove
    et al.
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Lundberg, Emma
    KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Uhlén, Mathias
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Introducing the Affinity Binder Knockdown Initiative-A public-private partnership for validation of affinity reagents2016In: EuPA Open Proteomics, ISSN 0014-2328, E-ISSN 2212-9685, Vol. 10, p. 56-58Article in journal (Refereed)
    Abstract [en]

    The newly launched Affinity Binder Knockdown Initiative encourages antibody suppliers and users to join this public-private partnership, which uses crowdsourcing to collect characterization data on antibodies. Researchers are asked to share validation data from experiments where gene-editing techniques (such as siRNA or CRISPR) have been used to verify antibody binding. The initiative is launched under the aegis of Antibodypedia, a database designed to allow comparisons and scoring of publicly available antibodies towards human protein targets. What is known about an antibody is the foundation of the scoring and ranking system in Antibodypedia.

  • 4140.
    Alm, Tove
    et al.
    KTH, School of Biotechnology (BIO), Proteomics.
    Nilvebrant, Johan
    KTH, School of Biotechnology (BIO), Proteomics.
    Hober, Sophia
    KTH, School of Biotechnology (BIO), Proteomics.
    Löfblom, John
    KTH, School of Biotechnology (BIO), Proteomics.
    Engineering bispecificityinto a single albumin-binding domain aimed for drug-targeting and extended in vivo half-life extension.Manuscript (preprint) (Other academic)
  • 4141.
    Alm, Tove
    et al.
    KTH, School of Biotechnology (BIO), Proteomics.
    Steen, Johanna
    KTH, School of Biotechnology (BIO), Proteomics.
    Ottosson, Jenny
    KTH, School of Biotechnology (BIO), Proteomics.
    Hober, Sophia
    KTH, School of Biotechnology (BIO), Proteomics.
    High-throughput protein purification under denaturating conditions by the use of cation exchange chromatography2007In: Biotechnology Journal, ISSN 1860-6768, Vol. 2, p. 709-716Article in journal (Refereed)
    Abstract [en]

    A high-throughput protein purification strategy using the polycationic Z(basic) tag has been developed. In order for the strategy to be useful both for soluble and less soluble proteins, a denaturating agent, urea, was used in all purification steps. First, four target proteins were genetically fused to the purification tag, Z(basic). These protein constructs were purified by cation exchange chromatography and eluted using a salt gradient. From the data achieved, a purification strategy was planned including stepwise elution to enable parallel protein purification using a laboratory robot. A protocol that includes all steps, equilibration of the chromatography resin, load of sample, wash, and elution, all without any manual handling steps, was handled by the laboratory robot. The program allows automated purification giving milligram amounts of pure recombinant protein of up to 60 cell lysates. In this study 22 different protein constructs, with different characteristics regarding pI and solubility, were successfully purified by the laboratory robot. The data show that Z(basic) can be used as a general purification tag also under denaturating conditions. Moreover, the strategy enables purification of proteins with different pI and solubility using ion exchange chromatography (IEXC). The procedure is highly reproducible and allows for high protein yield and purity and is therefore a good complement to the commonly used His(6)-tag.

  • 4142.
    Alm, Tove
    et al.
    KTH, Centres, Science for Life Laboratory, SciLifeLab.
    von Feilitzen, Kalle
    KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Lundberg, Emma
    KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Sivertsson, Åsa
    KTH, Centres, Science for Life Laboratory, SciLifeLab.
    Uhlén, Mathias
    KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
    A Chromosome-Centric Analysis of Antibodies Directed toward the Human Proteome Using Antibodypedia2014In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 13, no 3, p. 1669-1676Article in journal (Refereed)
    Abstract [en]

    Antibodies are crucial for the study of human proteins and have been defined as one of the three pillars in the human chromosome-centric Human Proteome Project (CHPP). In this article the chromosome-centric structure has been used to analyze the availability of antibodies as judged by the presence within the portal Antibodypedia, a database designed to allow comparisons and scoring of publicly available antibodies toward human protein targets. This public database displays antibody data from more than one million antibodies toward human protein targets. A summary of the content in this knowledge resource reveals that there exist more than 10 antibodies to over 70% of all the putative human genes, evenly distributed over the 24 human chromosomes. The analysis also shows that at present, less than 10% of the putative human protein-coding genes (n = 1882) predicted from the genome sequence lack antibodies, suggesting that focused efforts from the antibody-based and mass spectrometry-based proteomic communities should be encouraged to pursue the analysis of these missing proteins. We show that Antibodypedia may be used to track the development of available and validated antibodies to the individual chromosomes, and thus the database is an attractive tool to identify proteins with no or few antibodies yet generated.

  • 4143.
    Alm, Tove
    et al.
    KTH, School of Biotechnology (BIO), Proteomics.
    Yderland, Louise
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Nilvebrant, Johan
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Halldin, Anneli
    KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
    Hober, Sophia
    KTH, School of Biotechnology (BIO), Proteomics.
    A small bispecific protein selected for orthogonal affinity purification2010In: BIOTECHNOL J, ISSN 1860-6768, Vol. 5, no 6, p. 605-617Article in journal (Refereed)
    Abstract [en]

    A novel protein domain with dual affinity has been created by randomization and selection. The small alkali-stabilized albumin-binding domain (ABD(star)), used as scaffold to construct the library, has affinity to human serum albumin (HSA) and is constituted of 46 amino acids of which 11 were randomized. To achieve a dual binder, the binding site of the inherent HSA affinity was untouched and the randomization was made on the opposite side of the molecule. Despite its small size and randomization of almost a quarter of its amino acids, a bifunctional molecule, ABDz1, with ability to bind to both HSA and the Z(2) domain/protein A was successfully selected using phage display. Moreover, the newly selected variant showed improved affinity for HSA compared to the parental molecule. This novel protein domain has been characterized regarding secondary structure and affinity to the two different ligands. The possibility for affinity purification on two different matrices has been investigated using the two ligands, the HSA matrix and the protein A-based, MabSelect SuRe matrix, and the new protein domain was purified to homogeneity. Furthermore, gene fusions between the new domain and three different target proteins with different characteristics were made. To take advantage of both affinities, a purification strategy referred to as orthogonal affinity purification using two different matrices was created. Successful purification of all three versions was efficiently carried out using this strategy.

  • 4144.
    Alm, Victor
    KTH, School of Industrial Engineering and Management (ITM), Machine Design (Dept.).
    Affärsnätverk och deras betydelse för små tillmedelstora teknikföretag i miljöteknikbranschen2010Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The awareness of climate change and notions of the environmental impacts of burning fossilfuels has penetrated the society in recent years. This has raised a need for new, sustainabletechnologies that make it possible to slow down the expected temperature rise and followingclimate change of the planet.The industry for environmental technology is expected to grow rapidly in the coming years. InSweden there is a history of developing sustainable and efficient solutions improving theenvironment, whether it is the working conditions in a factory, air environment indoors,sustainable energy production or reducing hazardous exhaust fumes from incineration plants.The Swedish environmental technology industry is growing and a majority of the firms areSMEs that have great potential to become successful on the global market. But to reachinternational customers there is a barrier. This barrier if often connected with putting largeefforts, both monetary and human into penetrating these markets. The risks correlated with theseefforts impede many SMEs and instead they choose to look for other ways to grow.The purpose of this master thesis has been to explore how Swedish firms in environmentaltechnology use their business network and inter-organizational collaboration to gain competitiveadvantages and stimulate growth. The research has been exploratory with an aim to gain betterinsights how SME’s shape their strategies and utilize networks and relationships with otheractors to expand their business.11 Swedish environmental technology SMEs have been interviewed regarding how theycollaborate with other firms, what type of networks they utilize to gain competitiveness andupsize their business and their interest and activities on emerging markets. Based on the resultstwo firms where chosen for a multiple case study where their product and business developmentprocess and strategy were analysed.The results show that business/strategic networks are quintessential for SME’s and all firmsagreed that networks improve the competitiveness of the SME (Telephone interviews). Thestrategic network usually is a construction of several smaller networks, nets. These have clearlydefined goals and each net develops a structure that corresponds to the goals. The nets vary intheir nature, time-scope and structure and connect a firm with other firms, vertically oriiihorizontally. These nets could facilitate SMEs to act big together and support the creation of atotal solution.The conclusions show that successful export of environmental technology products and servicesrequires patience, relationships and an ability to become local on any market. To facilitate theseactions the SME must be establish a strong strategic network to depend on. I believe there is aneed for efficient tools and support for SMEs to develop and understand these networks. Newrelationships are formed and others end - just like in real life - and monitoring your businessnetwork can allow you to better predict what could happen tomorrow.

  • 4145.
    Al-Maawali, Hanna
    KTH, School of Engineering Sciences (SCI), Physics.
    Fabrication of channel waveguides in LiNb03.2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    In this bachelor’s thesis, a fabrication process for channel waveguides in LiNbO

    3crystals using the proton exchange technique is developed and characterized. These waveguides can be used at the laser physics department at the Royal Institution of Technology, KTH, for specifically non-linear optical applications.

    A waveguide is a device that guides a wave. In this case an optical waveguide is fabricated and so it guides electromagnetic waves in the optical spectrum that is light. To guide the wave diffraction has to be prevented in one or two dimensions, constraining the light to travel along a certain desired path. Waveguides can be fabricated in small sizes down to the micrometer level. The small structures can yield high-intensity guided waves with low input powers and this can lead to more efficient and compact nonlinear devices (sensitive to the field intensities). This makes it possible to produce compact and efficient devices with waveguides.

    To confine the light in the waveguide, the refractive index at the surface of the crystal will be increased creating a guiding layer in that region. The index increase is achieved by proton exchange. Proton exchange is a process where the lithium ions at the surface of the crystal are exchanged with hydrogen ions. This exchanged part makes the guiding layer because the hydrogen ions increase the refractive index of LiNbO

    3.

    The fabrication process of the channel waveguides consisted of transferring a mask pattern into the LiNbO

    3crystal. To do this, titanium was uniformly deposited on the crystal and on top of it a layer of photoresist was spun. The mask was transferred into the photoresist by photolithography and then etched into the titanium. The proton exchange could then take place in the mask openings.

    The progression of the fabrication was carefully documented after each step of the process to assess the quality of the waveguides. Several waveguides were fabricated on each sample with widths ranging from 2 to 10 microns.

    In the end, the full process for waveguide patterning and fabrication was developed on LiNbO

    3 substrates. The fabrication recipe developed in this work allowed for reliable fabrication of uniform channel waveguides over the whole sample length, L=12 mm, with widths down to 1.02 μm. A remarkably good result if one considers that this is beyond typical resolutions (~2μm) of the lithographic system used in this work.

  • 4146.
    Al-Maeeni, Lway
    KTH, School of Engineering Sciences (SCI), Physics.
    Sub-cooled nucleate boiling flow cooling experiment in a small rectangular channel2015Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
  • 4147.
    Al-Maeeni Lway, Bassim
    et al.
    KTH, School of Engineering Sciences (SCI), Physics.
    Tornmalm, Johan
    KTH, School of Engineering Sciences (SCI), Physics.
    Reactivity changes due to fuel bundle displacement in light water nuclear reactors2011Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 4148.
    Almaleh, Nabaz
    et al.
    KTH, School of Architecture and the Built Environment (ABE), Civil and Architectural Engineering, Structural Design and Bridges.
    Djuric, Milan
    KTH, School of Architecture and the Built Environment (ABE), Civil and Architectural Engineering, Structural Design and Bridges.
    Utformning av lådbalkbroar i högpresterande betong med extern förspänning2005Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
  • 4149. Almandoz-Gil, Leire
    et al.
    Welander, Hedvig
    Ihse, Elisabet
    Khoonsari, Payam Emami
    Musunuri, Sravani
    Lendel, Christofer
    KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.
    Sigvardson, Jessica
    Karlsson, Mikael
    Ingelsson, Martin
    Kultima, Kim
    Bergstrom, Joakim
    Low molar excess of 4-oxo-2-nonenal and 4-hydroxy-2-nonenal promote oligomerization of alpha-synuclein through different pathways2017In: Free Radical Biology & Medicine, ISSN 0891-5849, E-ISSN 1873-4596, Vol. 110, p. 421-431Article in journal (Refereed)
    Abstract [en]

    Aggregated alpha-synuclein is the main component of Lewy bodies, intraneuronal inclusions found in brains with Parkinson's disease and dementia with Lewy bodies. A body of evidence implicates oxidative stress in the pathogenesis of these diseases. For example, a large excess (30: 1, aldehyde: protein) of the lipid peroxidation end products 4-oxo-2-nonenal (ONE) or 4-hydroxy-2-nonenal (HNE) can induce alpha-synuclein oligomer formation. The objective of the study was to investigate the effect of these reactive aldehydes on alpha-synuclein at a lower molar excess (3: 1) at both physiological (7.4) and acidic (5.4) pH. As observed by size-exclusion chromatography, ONE rapidly induced the formation of alpha-synuclein oligomers at both pH values, but the effect was less pronounced under the acidic condition. In contrast, only a small proportion of alpha-synuclein oligomers were formed with low excess HNE-treatment at physiological pH and no oligomers at all under the acidic condition. With prolonged incubation times (up to 96 h), more alpha-synuclein was oligomerized at physiological pH for both ONE and HNE. As determined by Western blot, ONE-oligomers were more SDS-stable and to a higher-degree cross-linked as compared to the HNE-induced oligomers. However, as shown by their greater sensitivity to proteinase K treatment, ONE-oligomers, exhibited a less compact structure than HNE-oligomers. As indicated by mass spectrometry, ONE modified most Lys residues, whereas HNE primarily modified the His50 residue and fewer Lys residues, albeit to a higher degree than ONE. Taken together, our data show that the aldehydes ONE and HNE can modify alpha-synuclein and induce oligomerization, even at low molar excess, but to a higher degree at physiological pH and seemingly through different pathways.

  • 4150.
    Almandoz-Gil, Leire
    et al.
    Uppsala Univ, Dept Publ Hlth & Caring Sci, Mol Geriatr, SE-75185 Uppsala, Sweden..
    Welander, Hedvig
    Uppsala Univ, Dept Publ Hlth & Caring Sci, Mol Geriatr, SE-75185 Uppsala, Sweden..
    Ihse, Elisabeth
    Uppsala Univ, Dept Publ Hlth & Caring Sci, Mol Geriatr, SE-75185 Uppsala, Sweden..
    Khoonsari, Payam Emami
    Uppsala Univ, Dept Med Sci, SE-75185 Uppsala, Sweden..
    Musunuri, Sravani
    Uppsala Univ, Dept Med Sci, SE-75185 Uppsala, Sweden..
    Lendel, Christofer
    KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry.
    Sigvardson, Jessica
    BioArctic AB, Warfvinges Vag 35, SE-11251 Stockholm, Sweden..
    Karlsson, Mikael
    Uppsala Univ, Dept Engn Sci, SE-75121 Uppsala, Sweden..
    Ingelsson, Martin
    Uppsala Univ, Dept Publ Hlth & Caring Sci, Mol Geriatr, SE-75185 Uppsala, Sweden..
    Kultima, Kim
    Uppsala Univ, Dept Med Sci, SE-75185 Uppsala, Sweden..
    Bergstrom, Joakim
    Uppsala Univ, Dept Publ Hlth & Caring Sci, Mol Geriatr, SE-75185 Uppsala, Sweden..
    Low molar excess of 4-oxo-2-nonenal and 4-hydroxy-2-nonenal promote oligomerization of alpha-synuclein through different pathways (vol 110, pg 421, 2017)2018In: Free Radical Biology & Medicine, ISSN 0891-5849, E-ISSN 1873-4596, Vol. 117, p. 258-258Article in journal (Refereed)
80818283848586 4101 - 4150 of 124192
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