The human blood proteome provides a holistic readout of health states through the assessment of thousands of circulating proteins. In this study, we present a pan-disease resource to enable the study of diverse disease phenotypes within a harmonized proteomics dataset. By profiling protein concentrations across 59 diseases and healthy cohorts, we identified proteins associated with age, sex, and body mass index, as well as disease-specific signatures. This study highlights shared and distinct protein patterns across conditions, demonstrating the power of a unified proteomics approach to uncover biological insights. The dataset, covering 8262 individuals and up to 5416 proteins, serves as an online resource for exploring disease-specific protein profiles and advancing precision medicine research.

Background: Escherichia coli ST131 and clade H30Rx are the most prevalent extended-spectrum β-lactamase-producing E. coli (ESBL-EC) causing bacteremia and urinary tract infections globally and in Sweden. Previous studies have linked ST131-H30Rx with septic shock and mortality, as well as prolonged carriage. In our previous study, ST131 constituted 54% of all ESBL-EC bacteremia originating from the urinary tract. Method: Utilizing whole-genome sequencing, we retrospectively compared virulence factors (VFs) and patient outcomes based on medical records among 77 isolates of ESBL-EC from 76 patients with pyelonephritis between 2009 and 2018 in a Swedish county. Results: The VFs Ibes and uropathogenic specific protein were associated with ST131 of all clades (p < 0.0001). Serine protease (p < 0.0001) and cnf1 (p = 0.0003) were more common among ST131-H30Rx compared to non-ST131 isolates whereas enterobactin and iss were more common among ST131-H30Rx compared to both other ST131 isolates (p < 0.0001 and p = 0.0007, respectively) and non-ST131 isolates (p < 0.0001). Sepsis within 36 h was less common among patients infected with ST131-H30Rx (p = 0.038). Conclusions: ST131-H30Rx isolates carried VFs which were associated with recurrence but not uniformly to sepsis. In this explorative study, our results indicate that the ST131-H30Rx clade are not more prone to cause severe infection than other sequence types, but prone to cause recurrence, in addition to ESBL production which limits treatment options. Further studies are warranted to explore the mechanisms driving the success of ST131-H30Rx isolates in causing recurrent infections and colonization, and to form preventive measures.