A lab-in-a-fiber component was fabricated using an optical fiber and a fiber capillary. It was used in a test suspension of fluorescently labeled and unlabeled cells and enabled detection of the labeled cells. Subsequently the labeled cells were selectively collected via suction into the capillary. A novel sampling technique reduced photobleaching of the labeled cells, extending the measurement time. The collected cells remained viable for downstream analysis. This platform’s low fabrication cost, simplicity, compatibility with standard laboratory equipment, and capacity for fully automated cell capture highlights its potential for future applications in minimally invasive sample collection and point-of-care diagnostics. We demonstrate this LiF device to showcase the capability of optical fiber technology in creating low-cost, low-complexity cancer diagnostic devices. Furthermore, the LiF device holds promise for in vivo diagnostics, facilitating cell isolation and analysis.

Ever since the mid-1960’s, locking the phases of modes enabled the generation of laser pulses of duration limited only by the uncertainty principle, opening the field of ultrafast science. In contrast to conventional lasers, mode spacing in random lasers is ill-defined because optical feedback comes from scattering centres at random positions, making it hard to use mode locking in transform limited pulse generation. Here the generation of sub-nanosecond transform-limited pulses from a mode-locked random fibre laser is reported. Rayleigh backscattering from decimetre-long sections of telecom fibre serves as laser feedback, providing narrow spectral selectivity to the Fourier limit. The laser is adjustable in pulse duration (0.34–20 ns), repetition rate (0.714–1.22 MHz) and can be temperature tuned. The high spectral-efficiency pulses are applied in distributed temperature sensing with 9.0 cm and 3.3 × 10−3 K resolution, exemplifying how the results can drive advances in the fields of spectroscopy, telecommunications, and sensing.

We present an optical fiber-based selective cell picking module capable of picking up and transferring single cells or clusters. Our Lab-in-a-fiber (LIF) module detects labelled cancer cells (MCF-7) and picks them up for further analysis.

Microfluidics has emerged rapidly over the past 20 years and has been investigated for a variety of applications from life sciences to environmental monitoring. Although continuous-flow microfluidics is ubiquitous, segmented-flow or droplet microfluidics offers several attractive features. Droplets can be independently manipulated and analyzed with very high throughput. Typically, microfluidics is carried out within planar networks of microchannels, namely, microfluidic chips. We propose that fibers offer an interesting alternative format with key advantages for enhanced optical coupling. Herein, we demonstrate the generation of monodisperse droplets within a uniaxial optofluidic Lab-in-a-Fiber scheme. We combine droplet microfluidics with laser-induced fluorescence (LIF) detection achieved through the development of an optical side-coupling fiber, which we term a periscope fiber. This arrangement provides stable and compact alignment. Laser-induced fluorescence offers high sensitivity and low detection limits with a rapid response time making it an attractive detection method for in situ real-time measurements. We use the well-established fluorophore, fluorescein, to characterize the Lab-in-a-Fiber device and determine the generation of similar to 0.9 nL droplets. We present characterization data of a range of fluorescein concentrations, establishing a limit of detection (LOD) of 10 nM fluorescein. Finally, we show that the device operates within a realistic and relevant fluorescence regime by detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products in the context of COVID-19 diagnostics. The device represents a step towards the development of a point- of-care droplet digital RT-LAMP platform.

We demonstrate the use of the electrooptic effect to control the propagation constant of the guided modes in silicate few mode fibers with internal electrodes. The electrooptic effect induces a perturbation of the fiber's refractive index profile that controls intermodal interference. To increase the electrooptic effect the silicate fibers are poled. The response time is in the nanosecond range. 

The electrooptic effect is used in a few-mode fiber to control intermodal interference. The fiber has internal electrodes and is poled to increase its electrooptic coefficient. The response time is in the nanosecond range. 

The electrooptic effect is used in a few-mode fiber to control intermodal interference. The fiber has internal electrodes and is poled to increase its electrooptic coefficient. The response time is in the nanosecond range.

Electrical corona discharge is employed in this work to deposit ions on the surface of an optical fiber, creating a strong electric field that is used for poling. Green laser light propagating in the core frees photocarriers that are displaced by the poling field. The technique presented can induce a higher optical nonlinearity than previously obtained in traditional optical poling with internal metal electrodes. To date, a maximum second order nonlinearity 0.13 pm/V has been achieved for a 15 kV corona discharge bias.

In this work, we report on a twin-core fiber sensor system that provides improved spectral efficiency, allows for multiplexing and gives low level of crosstalk. Pieces of the referred strongly coupled multicore fiber are used as sensors in a laser cavity incorporating a pulsed semiconductor optical amplifier (SOA). Each sensor has its unique cavity length and can be addressed individually by electrically matching the periodic gating of the SOA to the sensor's cavity roundtrip time. The interrogator acts as a laser and provides a narrow spectrum with high signal-to-noise ratio. Furthermore, it allows distinguishing the response of individual sensors even in the case of overlapping spectra. Potentially, the number of interrogated sensors can be increased significantly, which is an appealing feature for multipoint sensing.