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2019 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 14, no 4, article id e0216251Article in journal (Refereed) Published
Abstract [en]
Cell culture is a ubiquitous and flexible research method. However, it heavily relies on plastic consumables generating millions of tonnes of plastic waste yearly. Plastic waste is a major and growing global concern. Here we describe a new cell culture dish that offers a culture area equivalent to three petri dishes but that is on average 61% lighter and occupies 67% less volume. Our dish is composed of a lid and three thin containers surrounded by a light outer shell. Cell culture can be performed in each of the containers sequentially. The outer shell provides the appropriate structure for the manipulation of the dish as a whole. The prototype was tested by sequentially growing cells in each of its containers. As a control, sequential cultures in groups of 3 petri dishes were performed. No statistical differences were found between the prototype and the control in terms of cell number, cell viability or cell distribution.
Place, publisher, year, edition, pages
Public Library of Science, 2019
National Category
Environmental Engineering
Identifiers
urn:nbn:se:kth:diva-258015 (URN)10.1371/journal.pone.0216251 (DOI)000466364800040 ()31039189 (PubMedID)2-s2.0-85065404122 (Scopus ID)
Note
QC 20191004
2019-09-092019-09-092024-03-18Bibliographically approved