Infectious airborne particles can cause surgical site infections (SSIs). SSIs cause substantially increased morbidity and mortality as well as patients’ sufferings. A well-functioning ventila-tion system in an operating room (OR) plays an essential role in the prevention of SSIs. In this study, we applied Computational Fluid Dynamics (CFD) to evaluate the performance of a novel OR ventilation strategy – temperature controlled airflow (TAF) against two widely used OR ventilation systems: laminar airflow (LAF) and turbulent mixing airflow (TMA). The performance was measured in terms of airborne bacteria concentration. Simulation results confirmed the superiority of LAF and TAF to TMA in providing high air cleanliness and also showed that TAF can serve as an efficient alternative to LAF.