With about 12 million inhabitants, São Paulo, Brazil, is the largest city in South America. As in many other major southern hemisphere cities, this extreme concentration of people imposes a number of mobility and security challenges. The objective of this article was to investigate the space-time patterns of mobility and violent victimisation in São Paulo’s metro stations from a gender perspective. The methodology combines use of a Geographical Information System (GIS), statistical analysis through negative binomial regression modelling and hypothesis testing. Results indicate that mobility and the level of victimisation are gender dependent. Women are at higher risk of victimisation than men in São Paulo’s central metro station, while men run higher risk of violence at end stations – both notably during late night periods. The presence of employees reduces the risk of violence, except during the mornings. The article suggests that crime prevention initiatives need to be gender informed and sensitive to the particular spatial and temporal features of rapid transit environments.