A UV analysis method of valiolamine is provided to maximize detection sensitivity of valiolamine by making valiolamine to phenylisocyanate, and to enable accurate and quick HPLC analysis. CONSTITUTION: A UV analysis method of valiolamine comprises the steps of: deriving valiolamine in a microbial culture medium with a chromophore reagent to increase the detection sensitivity; and analyzing the valiolamine with increased detection sensitivity with a high performance liquid chromatography(HPLC)/UV detector. The microbial culture medium uses at least one of (i) glucose 50 g/L, soybean flour 36 g/L, peptone 5 g/L, CaCO3 4 g/L; (ii) glucose 20 g/L, corn starch 40 g/L, corn steep liquor 20 g/L, corn gluten meal 40 g/L, NH4Cl 5 g/L, NaCl 15 g/L, CaCO3 15 g/L; or (iii) glucose 10 g/L, soluble starch 50 g/L, peptone 15 g/L, corn gluten powder 30 g/L, NaCl 5 g/L, CaCO3 10 g/L. COPYRIGHT KIPO 2010

본 발명은 발리올아민의 UV 분석 방법에 관한 것으로, 본 발명의 구성은 발리올아민을 페닐이소시아네이트로 유도체화시켜 검출 민감도를 증진시킴으로써 고성능 액체 크로마토그래피(HPLC)/ UV 검출기로 분석하는 방법을 제공한다.