The North Sea Basin has been covered by ice sheets originating from both the British Isles and Scandinavia at multiple times during the Pleistocene. The Witch Ground Basin (WGB) in the central northern North Sea is a critical location in terms of interpreting Late Pleistocene glacial to glacimarine history of the North Sea since it was the location of the Witch Ground Ice Stream that was active on multiple occasions during the Mid to Late Pleistocene. We map five mega-scale glacial lineation flowsets corresponding to the changing ice flow direction of the Witch Ground Ice Stream and investigate the sedimentological fingerprint and corresponding subglacial depositional processes of this palaeo-ice stream. We show that sorted sand layers within a subglacial traction till represent periodic hydraulic jacking and ice–bed decoupling at the base of the Witch Ground Ice Stream. In contrast to previous studies that have described glacitectonites deposited below the most recent grounded ice in the WGB, we present analysis of sediment cores that recovered primarily massive diamictons without any obvious deformation structures. The most recent ice cover in the WGB (~18–16 ka) was thought to have been sourced from a localized ice cap over Orkney and Shetland. The presence of chalk clasts sourced from NW of the WGB described in this study from the stratigraphically youngest till confirms this interpretation. The transition from subglacial to glacimarine deposition, while acoustically well defined (from opaque to laminated acoustic units), appears surprisingly uniform in the recovered sediment cores, but can be differentiated based on a change in colour including mottling and banding, presence of whole intact shells, and the increased number of silt and sand lenses. 14^C dating of glacimarine muds indicate high sedimentation rates of between 80 and 260 cm ka⁻¹. The transition from glacimarine to marine deposition is represented by a comparative decrease in sedimentation rate and deposition of Holocene age sandy mud. This study demonstrates a highly dynamic Witch Ground Ice Stream in the northern North Sea during the Late Pleistocene with evolving subglacial hydrology and depositional processes at the ice stream bed that left a distinct geomorphological and sedimentological fingerprint within the WGB.