The current investigation intended to evaluate quantities that express the minimal cross section of the waist of the nerve fiber layer, to develop age adjusted tolerance intervals for not pathological, develop a strategy for sensitive longitudinal monitoring of glaucoma within an individual eye, to develop an intuitive patient record illustrating the monitored eye, and finally to apply the strategy for longitudinal monitoring of glaucoma on a prospective longitudinal cohort. Volumes of the optical nerve head were captured with the Topcon Triton system. Raw data were exported to a custom made deep learning system for segmentation of the minimal cross section of the waist of the nerve fiber layer in the optic nerve head (ONH). Then, the Pigment epithelium central limit-Inner limit of the retina–Minimal Distance (PIMD, two strategies), or Minimal Area (PIMA) was estimated angularly resolved in the frontal plane. Age adjusted reference levels for not pathological eye were derived from clinically not pathological eyes. Reference levels for progression were derived from the early to manifest glaucoma eye measured. Tolerance intervals for patient visit 1-3 were developed based on variability estimated in a not pathological population. Tolerance intervals for visit 4 were developed based on variability within the examined eye. There was insignificant difference between the two strategies for PIMD estimation. PIMA-angle should be limited to resolution in clock hrs. The developed patient record efficiently illustrated the relationship between estimate and progression with tolerance limit in early to manifest glaucoma eyes and a case of traumatic optical atrophy.