The scissor jump (SKJ) is vital in badminton, particularly for backcourt shots, but fatigue increases lower limb load and injury risk. This study investigates how quadriceps fatigue affects biomechanical characteristics and load during SKJ landing, aiming to understand its impact on injury risk. This study involved 27 amateur male badminton players from Ningbo University. Quadriceps fatigue was induced via knee exercises and footwork drills. Biomechanical data before (prior fatigue-PRF) and after fatigue (post fatigue-POF) were recorded using a force platform and motion capture system. Muscle activation was measured with EMG and analyzed through musculoskeletal modeling, with paired t-tests and SPM 1D (Statistical Parametric Mapping 1D) for statistical analysis. Under the POF condition, knee flexion angle increased, and power decreased (p < 0.001, p < 0.001, respectively); ankle plantarflexion angle increased, and power decreased (p < 0.001, p < 0.001, respectively). As fatigue progressed, joint reaction forces initially decreased but later increased. Joint energy dissipation decreased, with differences more pronounced in the coronal than sagittal plane. Achilles tendon force and anterior-posterior tibial shear force decreased, while coronal plane center-of-mass displacement increased. Findings show quadriceps fatigue harms limb stability, upping knee and ankle loads, disrupting the movement pattern, and risking coronal plane injuries. It is recommended that athletes enhance quadriceps endurance, improve neuromuscular control, and refine landing techniques to maintain stability and prevent injuries when fatigued.