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Expression of RuBisCO and PRK
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Industrial Biotechnology.
2025 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Uttryck av RuBisCO och PRK (Swedish)
Abstract [sv]

Molekylärbiologi öppnar upp möjligheter för introduktion av icke naturlig funktionalitet i organismer. Den här studien undersökte om ribulos-1,5-bisfosfatkarboxylas (RuBisCO) och fosforibulokinas (PRK) kunde introduceras, vilket skulle öppna upp stora möjligheter för framtida forskning. Nio RuBisCO varianter med hög karboxyleringshastighet, tillsammans med två varianter av PRK, lyckades transformeras. De här stammarna har potentiellt möjligheten att utnyttja CO2 som sekundärt substrat, vilket kan göra de mer effektiva för industriell produktion av små-molekyler.

Abstract [en]

Molecular biology allows for the possibility of engineering organisms to achieve functionality not found in nature. This study investigated whether Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and Phosphoribulokinase (PRK) could be introduced, opening up a wide variety of possibilities for future research. Nine variants of high carboxylation rate RuBisCOs combined with two variants of PRK were successfully introduced. These strains could potentially utilize CO2 as an additional substrate, possibly outperforming other strategies for efficient industrial production of small molecules.

Place, publisher, year, edition, pages
2025.
Series
TRITA-CBH-GRU ; 2025:188
Keywords [en]
Molecular biology, Ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO), Phosphoribulokinase (PRK), Enzymatic expression, Gene transformation
Keywords [sv]
Molekylärbiologi, ribulosbisfosfatkarboxylas-oxygenas (RuBisCO), Phosphoribulokinase (PRK), Enzymuttryck, Transformation
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-364641OAI: oai:DiVA.org:kth-364641DiVA, id: diva2:1969772
Subject / course
Biotechnology
Educational program
Master of Science - Industrial and Environmental Biotechnology
Supervisors
Examiners
Available from: 2025-06-16 Created: 2025-06-16 Last updated: 2025-06-17

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CiteExportLink to record
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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
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