We present two effective tools for computing the positive tropicalization of an algebraic variety. First, we outline conditions under which the initial ideal can be used to compute the positive tropicalization, offering a real analogue to the Fundamental Theorem of Tropical Geometry. Additionally, under certain technical assumptions, we provide a real version of the Transverse Intersection Theorem. Building on these results, we propose an algorithm to compute a combinatorial bound on the number of positive real roots of a system of parametrized polynomial equations. Furthermore, we discuss how this combinatorial bound can be applied to study the number of positive steady states of chemical reaction networks.