A highly selective and sensitive electrochemical biosensor was developed for the detection of Brucella abortus bacteria using a molecularly imprinted polymer (MIP) synthesized via electropolymerization of dopamine onto screen-printed gold electrodes. The fabrication process is simple, cost-effective, and suitable for scalable production. Morphological and structural characterization using Raman spectroscopy, field emission scanning electron microscopy (FESEM), and atomic force microscopy (AFM) confirmed a successful imprinting and uniform film formation. Electrochemical impedance spectroscopy (EIS) enabled sensitive detection, achieving a wide linear range from 10 to 106 CFU/mL, with a correlation coefficient (R2) of 0.9925, and a maximum ΔRct of 37.56 kΩ. Selectivity tests against closely related and non-target bacterial species, including Brucella melitensis, Brucella suis, Escherichia coli, and Salmonella spp., showed negligible cross-reactivity, confirming high molecular recognition for Brucella abortus species. The use of MIP-based sensor with other electroactive monomers [e.g., polyaniline, poly(pyrrole propionic acid), poly(pyrrole-2-carboxylic acid), and poly(2-aminophenol)] displayed significantly lower response compared to the suggested polydopamine-based MIP, probably due to their low conductivity, weak compatibility, and less favorable film properties. The developed technique has many advantages over methods based on bacterial culture, PCR, and ELISA, which are often expensive, time-consuming, and require specialized facilities. The developed sensor offered a high selectivity, rapid response, high stability, extended durability, robustness, universal applicability and design flexibility. These results highlight the potential of dopamine-based MIP/impedimetric sensor for practical Brucella abortus bacteria detection and supported their future integration into a portable diagnostic device for continuous real-sample analysis.