Nanofluids are stable dispersions of engineered nanometer-sized particles which have shown potential to enhance heat transfer properties. Nanoparticle morphology might influence the heat transport properties of the nanofluid. In this work our aim is to investigate the influence of nanoparticle morphology in the heat transfer and rheological properties of  ZnO nanofluids. ZnO nanoparticles and nanorods were synthesized and dispersed in ethylene glycol, as the base fluid, to obtain nanofluids with different percentages of nanoparticle loading. Ultrasonic agitation was used for obtaining a stable suspension and the use of surfactants was avoided. The concentrations of ZnO nanofluids were varied between 1 wt% and 3 wt%. The physicochemical properties of nanofluids were characterized by using various techniques including particle size analyzer, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FT-IR). The thermal conductivity of prepared nanofluids were measured by Transient Hot Wire (THW) method and our finding on the physicochemical, transport and rheological properties of the ZnO nanofluids, containing nanoparticles with different morphology, are presented in detail.