Multi-storey timber buildings can be built adopting a post and beam system made of glulam. A typically adopted connection for such structures is that which make use of slotted-in steel plates and dowels. Even though such a type of connection is able to transfer large loads, it has a number of deficiencies. Besides being relatively costly in terms of both materials and manufacturing, connections with slotted-in steel plates and dowels requires a very high level of accuracy both in manufacture and in construction. By replacing steel plates and dowels with gusset plates of e.g. plywood and screws, respectively, there are a number of possible benefits including ease of manufacture, economy and environmental issues. This paper presents a comprehensive experimental investigation on the load bearing capacity of post-beam connection consisting of slotted-in plywood plates in combination with either full-threaded self-tapping screws or small-diameter dowels. The investigation also includes a study on the influence of the plywood plate's orientation in relation to the direction of load. Test results and hand calculations have confirmed that beam-column connections with gusset plates of birch plywood, may reach sufficient capacities for realistic design in the Ultimate Limit State (ULS). By utilizing both external and slotted-in gusset plates of plywood, one could reach the same capacity and stiffness as a slotted-in steel plate connection. To further reduce the amount of steel in the connections, results showed that prefabricated glued columns with plywood can be an opportunity.