In this study, a mesoporous carbon-based electrochemical sensor has been made up, possessing the novelty for ultra-sensitive detecting Prasugrel in pharmaceutical formulation and biological samples. Mesoporous carbon has even and tunable pore size, greater surface area, with excellent, electrical conductivity, wide availability and also have unique surface functionalization properties which make them significant sensing material now a days. Determination of prasugrel was accomplished with mesoporous carbon/GCE sensor on square-wave and cyclic voltammetry. Effects of various environmental and instrumental parameters like effect of buffer, casting volume, solvent, and pH on peak signal have been optimized. The best results were obtained in 0.1% cetyltrimethylammonium bromide - aqueous 0.1 M potassium chloride–phosphate buffer (pH 5.3). The limit of detection and quantification of the pure drug was found to be 37.0 ng mL−1 and 110.0 ng mL−1 (with the correlation coefficient, r = 0.988 and the standard deviation, (S.D.) = 0.6 (n = 6), respectively. The fabricated sensor was efficaciously validated by the determination of the drug in pharmaceutical formulation. Recoveries obtained were 97.84–99.37%.